Detection of shigellae from stools of dysentery patients by culture and polymerase chain reaction techniques

Journal of Diarrhoeal Diseases Research

Volumn 16, Issue 4, 1998, Pages 248-251

  Islam, M S ^a   Hossain, M S ^a   Hasan, M K ^a   Rahman, M M ^a   Fuchs, G ^a   Mahalanabis, D ^b   Baqui, A H ^a   Albert, M J ^a  

^a SOCIETY FOR APPLIED STUDIES   (India)

^b ICDDRB Laboratory Sciences Division   (Bangladesh)

Author keywords

Bacillary; Comparative studies; Culture media; Dysentery; Polymerase chain reaction; Shigella

Indexed keywords

ARTICLE; BANGLADESH; CLINICAL TRIAL; COMPARATIVE STUDY; CULTURE MEDIUM; FECES; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGELLA; SHIGELLOSIS;

BACTERIOLOGICAL TECHNIQUES; BANGLADESH; BASE SEQUENCE; CULTURE MEDIA; DYSENTERY, BACILLARY; FECES; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGELLA;

EID: 0032233829     PISSN: 02538768     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Echevema P, Sethabutr O, Serichantalergs O, Lexomboon U, Tamura K. Shigella and enteroinvasive Escherichia coli infections in households of children with dysentery in Bangkok. J Infect Dis 1992;165:144-47.
2. Frankel G, Riley L, Giron JA, Valmassoi J, Friedmann A, Strockbine N, Falkow S, Schoolnik GK. Detection of Shigella in feces using DNA amplification. J Infect Dis 1990;161:1252-6.
3. Hartman AB, Venkatesan M, Oaks EV, Buyne JM. Sequence and molecular characterization of multicopy invasion plasmid antigen gene, ipati of Shigellaflexneri. J Bacterial 1990;1972:1905-15.
4. Hossain MA, Albert MJ, Hasan KHZ. Epidemiology of shigellosis in Teknaf, a coastal area of Bangladesh: a 10year survey. Epidemiol Infect 1990; 105:41-50.
5. Islam MS, Hasan MK, Miah MA, Sur GC, Felsenstein A, Venkatesan M, Sack RB, Albert MJ. Use of the polymerase chain reaction and fluorescent antibody methods for detecting viable but nonculturable Shigella dysenteriae type 1 in laboratory microcosms. Appl Environ Microbiol 1993;59:536-40.
6. Lampel KA, Jagow JA, Trucksess M, Hill WE. Polymerase chain reaction for detection of invasive Shigella flexneri in food. Appl Environ Microbiol 1990;56:153640.
7. Levine MM, DuPont HL, Formal SB, Hornick RB, Takeuch A, Gangaross EJ, Snyder MJ, Libonati JP. Pathogenesis ofShigella dysenteriae 1 (Shiga) dysentery. J Infect Dis 1973;127:261-70.
8. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-91.
9. Sethabutr O, Echeverria P, Hoge CW, Bodhidatta L, Pitarangsi C. Detection of Shigella and enteroinvasive Escherichia coli by PCR in the stools of patients with dysentery in Thailand.y£/ar/7joeo/DfJ A 1994;12:2659.
10. Steffan RJ, Atlas RM. DNA amplification to enhance detection of genetically engineered bacteria in environmental samples. Appl Environ Microbiol 1988;54:2185-91.