A tradeoff between protein stability and conformational mobility in homotrimeric dUTPases

FEBS Letters

Volumn 566, Issue 1-3, 2004, Pages 48-54

  Takács, Eniko ^a   Grolmusz, Vince K ^a   Vértessy, Beáta G ^a  

^a INSTITUTE OF ENZYMOLOGY   (Hungary)

Author keywords

, Imino dUTP, 2 deoxyuridine 5 ( , imino)triphosphate; CD, circular dichroism; DSC, differential scanning calorimetry; dUTPase, dUTP pyrophosphatase

Indexed keywords

DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE;

ARTICLE; BACTERIAL STRAIN; CIRCULAR DICHROISM; COMPARATIVE STUDY; DIFFERENTIAL SCANNING CALORIMETRY; EUKARYOTE; FLUORESCENCE; HYDROPHOBICITY; INFRARED SPECTROSCOPY; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE; TEMPERATURE DEPENDENCE; THREE DIMENSIONAL IMAGING;

ANIMALS; BACTERIAL PROTEINS; CALORIMETRY, DIFFERENTIAL SCANNING; CIRCULAR DICHROISM; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ENZYME STABILITY; ESCHERICHIA COLI; GUANIDINE; LIGANDS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PYROPHOSPHATASES; SPECTROMETRY, FLUORESCENCE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TEMPERATURE;

BACTERIA (MICROORGANISMS); EUKARYOTA; TRIXIS;

EID: 2442506930     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2004.04.039     Document Type: Article

References (21)

1. Shlomai J., Kornberg A. J. Biol. Chem. 253:1978;3305-33012
2. Goulian M., Bleile B.M., Dickey L.M., Grafstrom R.H., Ingraham H.A., Neynaber S.A., Peterson M.S., Tseng B.Y. Adv. Exp. Med. Biol. 195(Pt B):1986;89-95
3. Larsson G., Svensson L.A., Nyman P.O. Nat. Struct. Biol. 3:1996;532-538
4. Gonzalez A., Larsson G., Persson R., Cedergren-Zeppezauer E. Acta Crystallogr. D: Biol. Crystallogr. 57:2001;767-774
5. Fiser A., Vertessy B.G. Biochem. Biophys. Res. Commun. 279:2000;534-542
6. Dubrovay Z., Gàspári Z., Hunyadi-Gulyás É., Medzihradszky F.K., Perczel A., Vértessy B.G. J. Biol. Chem. 279:2004;17945-17950
7. Kovári J., et al. J. Biol. Chem. 279:2004;17932-17944
8. Persson R., Nord J., Roth R., Nyman P.O. Prep. Biochem. Biotechnol. 32:2002;157-172
9. Vertessy B.G., Persson R., Rosengren A.M., Zeppezauer M., Nyman P.O. Biochem. Biophys. Res. Commun. 219:1996;294-300
10. Vertessy B.G. Proteins. 28:1997;568-579
11. Privalov P.L., Potekhin S.A. Methods Enzymol. 131:1986;4-51
12. Larsson G., Nyman P.O., Kvassman J.O. J. Biol. Chem. 271:1996;24010-24016
13. Vertessy B.G., Larsson G., Persson T., Bergman A.C., Persson R., Nyman P.O. FEBS Lett. 421:1998;83-88
14. Nord J., Nyman P., Larsson G., Drakenberg T. FEBS Lett. 492:2001;228-232
15. Kovári, J., Imre, T., Szabó, P. and Vértessy, B.G. (2004) Nucleosides, Nucleotides Nucleic Acids (in press)
16. Cantor C.R., Schimmel P.R. 1980;W.H. Freeman and Company, San Francisco
17. Greenfield N.J. Anal. Biochem. 235:1996;1-10
18. Poklar N., Petrovcic N., Oblak M., Vesnaver G. Protein Sci. 8:1999;832-840
19. Privalov P.L., Makhatadze G.I. J. Mol. Biol. 224:1992;715-723
20. Susi H., Byler D.M. Methods Enzymol. 130:1986;290-311
21. Prasad G.S., Stura E.A., Elder J.H., Stout C.D. Acta Crystallogr. D: Biol. Crystallogr. 56:2000;1100-1109