Identification of genetically modified potato (Solanum tuberosum) cultivars using event specific polymerase chain reaction

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 17, 2005, Pages 6691-6696

  Côté, Marie José ^a   Meldrum, Allison J ^a   Raymond, Philippe ^a   Dollard, Cheryl ^a  

^a CANADIAN FOOD INSPECTION AGENCY   (Canada)

Author keywords

Event specific identification; Genetically modified potato; GMO; NewLeaf; NewLeaf Plus; NewLeaf Y; Plant border region; Polymerase chain reaction; Solanum tuberosum

Indexed keywords

PLANT DNA;

ARTICLE; CLASSIFICATION; DNA SEQUENCE; GENETICS; METHODOLOGY; POLYMERASE CHAIN REACTION; POTATO; SENSITIVITY AND SPECIFICITY; TRANSGENIC PLANT;

DNA, PLANT; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; SOLANUM TUBEROSUM;

SOLANUM TUBEROSUM;

EID: 24344436258     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf050591i     Document Type: Article

References (22)

1. Canadian Food Inspection Agency Internet site: http://www.inspection.gc. ca/english/plaveg/variet/pntvcne.shtml. http://www.inspection.gc.ca/english/ plaveg/bio/pntvcne.shtml.
2. Akiyama, H.; Sugimoto, K.; Matsumoto, M.; Isuzugawa, K.; Shibuya, M.; Goda, Y.; Toyoda, M. A detection method of recombinant DNA from genetically modified potato (NewLeaf Plus potato) and detection of NewLeaf Plus potato in snack. Shokuhin Eiseigaku Zasshi 2002, 43, 24-29.
3. Jaccaud, E.; Hohne, M.; Meyer, R. Assessment of screening methods for identification of genetically modified potatoes in raw materials and finished products. J. Agric. Food Chem. 2003, 51, 550-557.
4. Rho, J. K.; Lee, T.; Jung, S. I.; Kim, T. S.; Park, Y. H.; Kim, Y. M. Qualitative and quantitative PCR methods for detection of three lines of genetically modified potatoes. J. Agric. Food Chem. 2004, 52, 3269-3274.
5. Watanabe, T.; Kuribara, H.; Mishima, T.; Kikuchi, H.; Kodama, T.; Futo, S.; Kasama, K.; Toyota, A.; Nouno, M.; Saita, A.; Takahashi, K.; Hino, A.; Akiyama, H.; Maitani, T. New qualitative detection methods of genetically modified potatoes. Biol. Pharm. Bull. 2004, 27, 1333-1339.
6. BATS. Centre for Biosafety and Sustainability Internet site: http://www.gmo-watch.org/GVO-report140703.pdf.
7. Windels, P.; Taverniers, I.; Depicker, A.; Van Bockstaele, E.; De Loose, M. Characterisation of the Roundup Ready soybean insert. Eur. Food Res. Technol. 2001, 213, 107-112.
8. Spertini, D.; Béliveau, C.; Bellemare, G. Screening of transgenic plants by amplification of unknown genomic DNA flanking T-DNA. BioTechniques 1999, 27, 308-312.
9. Rudenko, G. N.; Rommens, C. M. T.; Nijkamp, H. J. J.; Hille, J. Supported PCR: An efficient procedure to amplify sequences flanking a known DNA segment. Plant Mol. Biol. 1993, 21, 723-728.
10. Myrick, K. V.; Gelbart, W. M. Universal Fast Walking for direct and versatile determination of flanking sequence. Gene 2002, 284, 125-131.
11. Depicker, A.; Stachel, S.; Dhaese, P.; Zambryski, P.; Goodman, H. M. Nopaline synthase: Transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1982, 1, 561-573.
12. Barker, R. F.; Idler, K. B,; Thompson, D. V.; Kemp, J. D. Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol. Biol. 1983, 2, 335-350.
13. Coruzzi, G.; Broglie, R.; Edwards, C.; Chua, N.-H. Tissue-specific and light-regulated expression of pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. EMBO J. 1984, 3, 1671-1679.
14. Bevan, M.; Barnes, W. M.; Chilton, M.-D. Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 1983, 11, 369-385.
15. Beck, E.; Ludwig, G.; Auerswald, E. A.; Reiss, B.; Schaller, H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 1982, 19, 327-336.
16. Richins, R.; Scholthof, H. B.; Shepherd, R. J. Sequence of figwort mosaic virus DNA (caulimovirus group). Nucleic Acids Res. 1987, 15, 8451-8466.
17. Binns, A. N.; Thomashow, M. F. Cell biology of Agrobacterium infection and transformation of plants. Annu. Rev. Microbiol. 1988, 42, 575-606.
18. Hernández, M.; Pla, M.; Esteve, T.; Prat, S.; Puigdomènech, P.; Ferrando, A. A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard based on the 3′-transgene integration sequence. Transgenic Res. 2003, 12, 179-189.
19. Windels, P.; Bertrand, S.; Depicker, A.; Moens, W.; Bockstaele, E.; Loose, M. Qualitative and event-specific PCR real-time detection methods for StarLink maize. Eur. Food Res. Technol., 2003, 216, 259-263.
20. Rønning, S. B.; Vaïtilingom, M.; Berdal, K. G.; Holst-Jensen, A. Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays). Eur. Food Res. Technol. 2003, 216, 347-354.
21. Huang, H.-Y.; Pan, T.-M. Detection of genetically modified maize MON810 and NK603 by multiplex and real-time polymerase chain reaction methods. J. Agric. Food Chem. 2004, 52, 3264-3268.
22. Nielsen, C. R.; Berdal, K. G.; Holst-Jensen, A. Characterisation of the 5′ integration site and development of an event-specific real-time PCR assay for NK603 maize form a low starting copy number. Eur. Food Res. Technol. 2004, 219, 421-427.