Single-molecule measurements calibrate green fluorescent protein surface densities on transparent beads for use with 'knock-in' animals and other expression systems

Journal of Neuroscience Methods

Volumn 105, Issue 1, 2001, Pages 55-63

  Chiu, Chi Sung ^a   Kartalov, Emil ^b   Unger, Marc ^b   Quake, Stephen ^b   Lester, Henry A ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b California Institute of Technology   (United States)

Author keywords

Calibration; Epi fluorescent microscope; GFP (green fluorescent protein); Histidine tag; pH sensitivity; Photobleaching rate; Single molecule imaging

Indexed keywords

GREEN FLUORESCENT PROTEIN; HISTIDINE; MEMBRANE PROTEIN;

AMINO ACID ANALYSIS; ANIMAL; ARTICLE; BLEACHING; CALIBRATION; CAMERA; CONTROLLED STUDY; DENSITY; FLUORESCENCE; FLUORESCENCE MICROSCOPE; GENE EXPRESSION; GENE INSERTION; IMAGING; INTERMETHOD COMPARISON; LIGHT; MEASUREMENT; MOLECULE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; SURFACE PROPERTY; SYNAPSE; SYNAPTIC TRANSMISSION; TECHNIQUE;

ANIMALS; DENSITOMETRY; GREEN FLUORESCENT PROTEINS; HISTIDINE; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MICROSPHERES; MODELS, ANIMAL; PHOTOCHEMISTRY; SYNAPTIC MEMBRANES; SYNAPTIC TRANSMISSION;

EID: 0035970084     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(00)00354-X     Document Type: Article

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