Recombinant protein production by the baculovirus-insect cell system in basal media without serum supplementation

Cytotechnology

Volumn 43, Issue 1-3, 2003, Pages 3-10

  Nishikawa, Norikatsu ^a   Yamaji, Hideki ^a   Fukuda, Hideki ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Baculovirus; Insect cells; Recombinant protein production; Serum free culture; TNM FH

Indexed keywords

BETA GALACTOSIDASE; BOVINE SERUM ALBUMIN; LACTALBUMIN; PHOSPHATIDIC ACID; PHOSPHATIDYLCHOLINE; RECOMBINANT PROTEIN;

ADAPTATION; ANIMAL CELL; AUTOGRAPHA CALIFORNICA; BACULOVIRUS EXPRESSION SYSTEM; CANCER STAGING; CELL CULTURE; CELL GROWTH; CHO CELL; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; EGG YOLK; INSECT CELL; NONHUMAN; PLAQUE FORMING CELL; PROTEIN SYNTHESIS; YEAST;

AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; BOVINAE; CRICETULUS GRISEUS; INSECTA;

EID: 23944471871     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:CYTO.0000039894.27256.0f     Document Type: Conference Paper

References (16)

1. Cruz P.E., Martins P.C., Alves P.M., Peixoto C.C., Santos H., Moreira J.L. and Carrondo M.J.T. 1999. Proteolytic activity in infected and noninfected insect cells: degradation of HIV-1 Pr55gag particles. Biotechnol. Bioeng. 65: 133-143.
2. Donaldson M.S. and Shuler M.L. 1998. Low-cost serum-free medium for the BTI-Tn5B1-4 insect cell line. Biotechnol. Prog. 14: 573-579.
3. Gotoh T., Miyazaki Y., Sato W., Kikuchi K. and Bentley W. 2001. Proteolytic activity and recombinant protein production in virus-infected Sf-9 insect cell cultures supplemented with carboxyl and cysteine protease inhibitors. J. Biosci. Bioeng. 92: 248-255.
4. Hink W.F. 1970. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature 226: 466-467.
5. Jesionowski G.A. and Ataai M.M. 1997. An efficient medium for high protein production in the insect cell/baculovirus expression system. Biotechnol. Prog. 13: 355-360.
6. Licari P. and Bailey J.E. 1991. Factors influencing recombinant protein yields in an insect cell-baculovirus expression system: multiplicity of infection and intracellular protein degradation. Biotechnol. Bioeng. 37: 238-246.
7. Maiorella B., Inlow D., Shauger A. and Harano D. 1988. Large-scale insect cell-culture for recombinant protein production. Bio/Technology 6: 1406-1410.
8. Sakai K., Hayashi C., Yamaji H. and Fukuda H. 2001. Use of nonionic surfactants for effective supply of phosphatidic acid in serum-free culture of Chinese hamster ovary cells. J. Biosci. Bioeng. 92: 256-261.
9. Sakai K., Matsunaga T., Hayashi C., Yamaji H. and Fukuda H. 2002. Effects of phosphatidic acid on recombinant protein production by Chinese hamster ovary cells in serum-free culture. Biochem. Eng. J. 10: 85-92.
10. Sakai K., Matsunaga T., Yamaji H. and Fukuda H. 1999. Effects of phospholipids on growth of Chinese hamster ovary cells in serum-free media. J. Biosci. Bioeng. 88: 306-309.
11. Silberklang M., Ramasubramanyan K., Gould S.L., Lenny A.B., Seamans T.C., Wang S., Hunt G.R., Junker B., Mazina K.E., Tota M.R., Palyha O. and Jain D. 1995. Baculovirus-mediated production of proteins in insect cells: examples of scale-up and product recovery. In: Shuler M.L., Wood H.A., Granados R.R. and Hammer D.A. (eds), Baculovirus Expression Systems and Biopesticides. Wiley-Liss, New York, pp. 205-231.
12. Wu J., King G., Daugulis A.J., Faulkner P., Bone D.H. and Goosen M.F.A. 1989. Engineering aspects of insect cell suspension culture: a review. Appl. Microbiol. Biotechnol. 32: 249-255.
13. Yamaji H., Hirakawa D., Tagai S. and Fukuda H. 2003. Production of protein kinase C-δ by the baculovirus-insect cell system in serum-supplemented and serum-free media. J. Biosci. Bioeng. 95: 185-187.
14. Yamaji H., Tagai S. and Fukuda H. 1999. Optimal production of recombinant protein by the baculovirus-insect cell system in shake-flask culture with medium replacement. J. Biosci. Bioeng. 87: 636-641.
15. Yamaji H., Tagai S., Sakai K., Izumoto E. and Fukuda H. 2000. Production of recombinant protein by baculovirus-infected insect cells in immobilized culture using porous biomass support particles. J. Biosci. Bioeng. 89: 12-17.
16. Yang J.-D., Gecik P., Collins A., Czarnecki S., Hsu H.-H., Lasdun A., Sundaram R., Muthukumar G. and Silberklang M. 1996. Rational scale-up of a baculovirus-insect cell batch process based on medium nutritional depth. Biotechnol. Bioeng. 52: 696-706.