An efficient medium for high protein production in the insect cell/baculovirus expression system

Biotechnology Progress

Volumn 13, Issue 4, 1997, Pages 355-360

  Jesionowski, Gary A ^a   Ataai, Mohammad M ^a  

^a University of Pittsburgh   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BYPRODUCTS; ENZYMES; GROWTH KINETICS; TOXICITY;

BACULOVIRUS EXPRESSION SYSTEM; CELL INFECTION; EFFICIENT MEDIUM FOR INSECT CELL GROWTH (EMICG); INSECT CELL CULTURE;

ANIMAL CELL CULTURE;

EID: 0031194397     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp970054l     Document Type: Article

References (18)

1. Bedard, C.; Kamen, A.; Tom, R.; Massie, B. Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures. Cytotechnology 1994, 15, 129-138.
2. Caron, A. W.; Archambault, J.; Massie, B. High-level recombinant protein production in bioreactors using the baculovirus-insect cell expression system. Biotechnol. Bioeng. 1990, 36, 1133-1140.
3. Ferrance, J.; Goel, A.; Ataai, M. M. Utilization of glucose and amino acids in insect cell cultures: quantifying the metabolic flows within the primary pathways and medium development. Biotechnol. Bioeng. 1993, 42, 697-707.
4. Grace, T. D. C. Establishment of four strains of cells from insect tissues grown in vitro. Nature 1962, 195, 788-789.
5. Hink, W. F.; Thomsen, D. R.; Davidson, D. J.; Meyer, A. L.; Castellino, F. J. Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnol. Prog. 1991, 7, 9-14.
6. Lazarte, J. E.; Tosi, P.; Nicolau, C. Optimization of the production of full-length rCD4 in baculovirus-infected Sf9 cells. Biotechnol. Bioeng. 1992, 40, 214-217.
7. Licari, P.; Bailey, J. E. Factors influencing recombinant protein yields in an insect cell-baculovirus expression system: multiplicity of infection and intracellular protein degradation. Biotechnol. Bioeng. 1991, 37, 238-246.
8. Lindsay, D. A.; Betenbaugh, M. J. Quantification of cell culture factors affecting recombinant protein yields in baculovirus-infected insect cells. Biotechnol. Bioeng. 1992, 39, 614-618.
9. Luckow, V. A.; Summers, M. D. Trends in the development of baculovirus expression vectors. Bio/Technology 1988, 6, 47-55.
10. Maiorella, B.; Inlow, D.; Shauger, A.; Harano, D. Large-scale insect cell-culture for recombinant protein production. Bio/ Technology 1988, 6, 1406-1410.
11. McIntosh, A. H.; Grasela, J. J. Expression of β-galactosidase and luciferase in insect cell lines infected with a recombinant AcMNPV. In Vitro Cell. Dev. Biol. 1994, 30A, 275-278.
12. Miller, J. H. In Experiments in Molecular Genetics; Cold Spring Harbor: New York, 1972; pp 352-355.
13. Reuveny, S.; Kim, Y. J.; Kemp, C. W.; Shiloach, J. Production of recombinant proteins in high-density insect cell cultures. Biotechnol. Bioeng. 1993, 42, 235-239.
14. Wang, M. Y.; Kwong, S.; Bentley, W. E. Effects of oxygen/ glucose/glutamine feeding on insect cell baculovirus protein expression: a study on epoxide hydrolase production. Biotechnol. Prog. 1993, 9, 355-361.
15. Weiss, S. A.; Smith, G. C.; Kalter, S. S.; Vaughn, J. L. Improved method for the production of insect cell cultures in large volume. In Vitro 1981, 17, 495-502.
16. Wickham, T. J.; Davis, T.; Granados, R. R.; Hammer, D. A.; Shuler, M. L.; Wood, H. A. Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of multiplicity of infection. Biotechnol. Lett. 1991, 13, 483-488.
17. Wickham, T. J.; Davis, T.; Granados, R. R.; Shuler, M. L.; Wood, H. A. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system. Biotechnol. Prog. 1992, 8, 391-396.
18. Wong, K. T.; Peter, C. H.; Greenfield, P. F.; Reid, S.; Nielsen, L. K. Low multiplicity infection of insect cells with a recombinant baculovirus: the cell yield concept. Biotechnol. Bioeng. 1996, 49, 659-666.