Optimal production of recombinant protein by the baculovirus-insect cell system in shake-flask culture with medium replacement

Journal of Bioscience and Bioengineering

Volumn 87, Issue 5, 1999, Pages 636-641

  Yamaji, Hideki ^a   Tagai, Shin Ichi ^a   Fukuda, Hideki ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Baculovirus; Insect cells; Multiplicity of infection; Nutrient limitation; Recombinant protein production; Shake flask culture

Indexed keywords

RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL CULTURE; CELL DENSITY; GENETIC RECOMBINATION; INSECT; NONHUMAN; PROTEIN EXPRESSION;

ANIMALIA; BOVINAE; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0032974082     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(99)80127-3     Document Type: Article

References (12)

1. 1. Luckow, V. A.: Protein production and processing from baculovirus expression vectors, p. 51-90. In Shuler, M. L., Wood, H. A., Granados, R. R. and Hammer, D. A. (ed.), Baculovirus expression systems and biopesticides. John Wiley & Sons, New York (1995).
2. 2. Licari, P. and Bailey, J. E.: Modeling the population dynamics of baculovirus-infected insect cells: optimizing infection strategies for enhanced recombinant protein yields. Biotechnol. Bioeng., 39, 432-441 (1992).
3. 3. Power, J. F., Reid, S., Radford, K. M., Greenfield, P. F., and Nielsen, L. K.: Modeling and optimization of the baculovirus expression vector system in batch suspension culture. Biotechnol. Bioeng., 44, 710-719 (1994).
4. 4. Taticek, R. A., Hammer, D. A., and Shuler, M. L.: Overview of issues in bioreactor design and scale-up, p. 131-174. In Shuler, M. L., Wood, H. A., Granados, R. R., and Hammer, D. A. (ed.), Baculovirus expression systems and biopesticides. John Wiley & Sons, New York (1995).
5. 5. Wong, K. T. K., Peter, C. H., Greenfield, P. F., Reid, S., and Nielsen, L. K.: Low multiplicity infection of insect cells with a recombinant baculovirus: the cell yield concept. Biotechnol. Bioeng., 49, 659-666 (1996).
6. 6. Taticek, R. A. and Shuler, M. L.: Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system. Biotechnol. Bioeng., 54, 142-152 (1997).
7. 7. Bédard, C., Kamen, A., Tom, R., and Massie, B.: Maximization of recombinant protein yield in the insect cell/baculovirus system by one-time addition of nutrients to high-density batch cultures. Cytotechnology, 15, 129-138 (1994).
8. 8. Radford, K. M., Reid, S., and Greenfield, P. F.: Substrate limitation in the baculovirus expression vector system. Biotechnol. Bioeng., 56, 32-44 (1997).
9. 9. Hink, W. F.: Established insect cell line from the cabbage looper, Trichoplusia ni. Nature, 226, 466-467 (1970).
10. 10. Yang, J.-D., Gecik, P., Collins, A., Czarnecki, S., Hsu, H.-H., Lasdun, A., Sundaram, R., Muthukumar, G., and Siberklang, M.: Rational scale-up of a baculovirus-insect cell batch process based on medium nutritional depth. Biotechnol. Bioeng., 52, 696-706 (1996).
11. 11. Yamaji, H., Fukuda, H., Nojima, Y., and Webb, C.: Immobilization of anchorage-independent animal cells using reticulated polyvinyl formal resin biomass support particles. Appl. Microbiol. Biotechnol., 30, 609-613 (1989).
12. 12. Yamaji, H. and Fukuda, H.: Long-term cultivation of anchorage-independent animal cells immobilized within reticulated biomass support particles in a circulating bed fermentor. Appl. Microbiol. Biotechnol., 34, 730-734 (1991).