Effects of phospholipids on growth of Chinese hamster ovary cells in serum-free media

Journal of Bioscience and Bioengineering

Volumn 88, Issue 3, 1999, Pages 306-309

  Sakai, Kentaro ^a   Matsunaga, Toshio ^a   Yamaji, Hideki ^a   Fukuda, Hideki ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

CHO cells; Lysophosphatidic acid; MTT assay; Phosphatidic acid; Serum free medium

Indexed keywords

ANIMAL CELL CULTURE; BIOASSAY; COMPOSITION EFFECTS; LIPIDS; MOLECULAR STRUCTURE; UNSATURATED COMPOUNDS;

LYSOPHOSPHATIDIC ACID; PHOSPHATIDIC ACID;

PROTEINS;

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; DIOLEOYLPHOSPHATIDIC ACID; GROWTH PROMOTOR; INSULIN; LYSOPHOSPHATIDIC ACID; PHOSPHATIDIC ACID; PHOSPHATIDYLCHOLINE; PHOSPHOLIPID; RECOMBINANT PROTEIN; TRANSFERRIN;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; DNA SYNTHESIS; NONHUMAN; PROTEIN SYNTHESIS;

EID: 0033199942     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(00)80014-6     Document Type: Article

References (12)

1. 1. van Corven, E. J., Groenink, A., Jalink, K., Eichholtz, T., and Moolenaar, W. H.: Lysophosphatidate-induced cell proliferation: identification and dissection of signaling pathways mediated by G proteins. Cell, 59, 45-54 (1989).
2. 2. van Corven, E. J., van Rijswijk, A., Jalink, K., van der Bend, R. L., van Blitterswijk, W. J., and Moolenaar, W. H.: Mitogenic action of lysophosphatidic and phosphatidic acid on fibroblasts: dependence on acyl-chain length and inhibition by suramin. Biochem. J., 281, 163-169 (1992).
3. 3. Moolenaar, W. H.: Lysophosphatidic acid, a multifunctional phospholipid messenger. J. Biol. Chem., 270, 12949-12952 (1995).
4. 2+-mobilizing stimulus for fibroblasts: evidence for an extracellular site of action. J. Biol. Chem., 265, 12232-12239 (1990).
5. 5. Urlaub, G. and Chasin, L. A.: Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc. Natl. Acad. Sci. USA, 77, 4216-4220 (1980).
6. 6. Mizuguchi, M., Matsumoto, K., and Onodera, K.: Production of the human growth hormone in serum-free UC203 medium, p. 57-61. In Kaminogawa, S., Ametani, A., and Hachimura, S. (ed.), Animal cell technology: basic & applied aspects, vol. 5. Kluwer Academic Publishers, Dordrecht, The Netherlands (1993).
7. 7. Murakami, H., Masui, H., Sato, G. H., Sueoka, N., Chow, T. P., and Kano-Sueoka, T.: Growth of hybridoma cells in serum-free medium: ethanolamine is an essential component. Proc. Natl. Acad. Sci. USA, 79, 1158-1162 (1982).
8. 8. Mosmann, T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65, 55-63 (1983).
9. 9. Yamaji, H. and Fukuda, H.: Growth and death behaviour of anchorage-independent animal cells immobilized within porous support matrices. Appl. Microbiol. Biotechnol., 37, 244-251 (1992).
10. 10. Maiorella, B., Inlow, D., Shauger, A., and Harano, D.: Large-scale insect cell-culture for recombinant protein production. Bio/Technology, 6, 1406-1410 (1988).
11. 11. Martial, A., Gaillard, I., Engasser, J.-M., and Marc, A.: Continuous hybridoma culture in a low-protein serum-free medium supplemented with liposomes. Enzyme Microb. Technol., 17, 1062-1066 (1995).
12. 12. Minamoto, Y., Ogawa, K., Abe, H., Iochi, Y., and Mitsugi, K.: Development of a serum-free and heat-sterilizable medium and continuous high-density cell culture. Cytotechnology, 5, S35-51 (1991).