Immunohistochemical analysis of MCT1, MCT2 and MCT4 expression in rat plantaris muscle

Journal of Physiology

Volumn 567, Issue 1, 2005, Pages 121-129

  Hashimoto, Takeshi ^a,b   Masuda, Shinya ^a   Taguchi, Sadayoshi ^a   Brooks, George A ^b  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AVIDIN; BIOTIN; CYTOCHROME C OXIDASE; GLUCOSE TRANSPORTER 4; LACTIC ACID; MITOCHONDRIAL ENZYME; MONOCARBOXYLATE TRANSPORTER; MONOCARBOXYLATE TRANSPORTER 1; MONOCARBOXYLATE TRANSPORTER 2; MONOCARBOXYLATE TRANSPORTER 4; UNCLASSIFIED DRUG; MYOSIN ADENOSINE TRIPHOSPHATASE;

ANIMAL TISSUE; ARTICLE; FAST MUSCLE FIBER; GLYCOLYSIS; IMMUNOHISTOCHEMISTRY; INTRACELLULAR TRANSPORT; MALE; MUSCLE CELL; NONHUMAN; OXIDATION; PLANTARIS MUSCLE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SARCOLEMMA; SKELETAL MUSCLE; SLOW MUSCLE FIBER; WESTERN BLOTTING; ANIMAL EXPERIMENT; BIOCHEMISTRY; CELLULAR DISTRIBUTION; CELLULAR PARAMETERS; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; DOWN REGULATION; FAST GLYCOLYTIC FIBER; FAST OXIDATIVE GLYCOLYTIC FIBER; HISTOCHEMISTRY; MITOCHONDRIAL RETICULUM; PROTEIN ANALYSIS; PROTEIN FUNCTION; QUANTITATIVE ANALYSIS; SKELETAL MUSCLE CELL; SLOW OXIDATIVE FIBER; UPREGULATION;

ADENOSINE TRIPHOSPHATASES; ANIMALS; GLYCEROLPHOSPHATE DEHYDROGENASE; IMMUNOHISTOCHEMISTRY; MALE; MICROSCOPY, CONFOCAL; MONOCARBOXYLIC ACID TRANSPORTERS; MUSCLE FIBERS, FAST-TWITCH; MUSCLE FIBERS, SLOW-TWITCH; MUSCLE PROTEINS; MUSCLE, SKELETAL; RATS; RATS, WISTAR; SUCCINATE DEHYDROGENASE; SYMPORTERS;

EID: 23844526132     PISSN: 00223751     EISSN: None     Source Type: Journal    
DOI: 10.1113/jphysiol.2005.087411     Document Type: Article

References (35)

1. Benton CR, Campbell SE, Tonouchi M, Hatta H & Bonen A (2004). Monocarboxylate transporters in subsarcolemmal and intermyofibrillar mitochondria. Biochem Biophys Res Commun 323, 249-253.
2. Bergman BC, Wolfel EE, Butterfield GE, Lopaschuk GD, Casazza GA, Horning MA & Brooks GA (1999). Active muscle and whole body lactate kinetics after endurance training in men. J Appl Physiol 87, 1684-1696.
3. Bonen A (2001). The expression of lactate transporters (MCT1 and MCT4) in heart and muscle. Eur J Appl Physiol 86, 6-11.
4. Bröer S, Bröer A, Schneider HP, Stegen C, Halestrap AP & Deitmer JW (1999). Characterization of the high-affinity monocarboxylate transporter MCT2 in Xenopus laevis oocytes. Biochem J 341 (3), 529-535.
5. Brooks GA (1998). Mammalian fuel utilization during sustained exercise. Comp Biochem Physiol B Biochem Mol Biol 120, 89-107.
6. Brooks GA (2002). Lactate shuttles in nature. Biochem Soc Trans 30, 259-264.
7. Brooks GA, Brown MA, Butz CE, Sicurello JP & Dubouchaud H (1999b). Cardiac and skeletal muscle mitochondria have a monocarboxylate transporter MCT1. J Appl Physiol 87, 1713-1718.
8. Brooks GA, Dubouchaud H, Brown MA, Sicurello JP & Butz CE (1999a). Role of mitochondrial lactate dehydrogenase and lactate oxidation in the 'intra-cellular lactate shuttle'. Proc Natl Acad Sci U S A 96, 1129-1134.
9. Brooks GA, Wolfel FE, Butterfield GE, Cymerman A, Roberts AC, Mazzeo RS & Reeves JT (1998). Poor relationship between arterial [lactate] and leg net release during exercise at 4300 m altitude. Am J Physiol 275, R1192-R1201.
10. Brown MA & Brooks GA (1994). Trans-stimulation of lactate transport from rat sarcolemmal membrane vesicles. Arch Biochem Biophys 313, 22-28.
11. Butz CE, McClelland GB & Brooks GA (2004). MCT1 confirmed in rat striated muscle mitochondria. J Appl Physiol 97, 1059-1066.
12. Dimmer KS, Friedrich B, Lang F, Deitmer JW & Broer S (2000). The low-affinity monocarboxylate transporter MCT4 is adapted to the export of lactate in highly glycolytic cells. Biochcm J 350, 219-227.
13. Dubouchaud H, Butterfield GE, Wolfel EE, Bergman BC & Brooks GA (2000). Endurance training, expression, and physiology of LDH, MCT1, and MCT4 in human skeletal muscle. Am J Physiol Endocrinol Metab 278, E571-E579.
14. Fishbein WN, Merezhinskaya N & Foellmer JW (2002). Relative distribution of three major lactate transporters in frozen human tissues and their localization in unfixed skeletal muscle. Muscle Nerve 26, 101-112.
15. Garcia CK, Brown MS, Pathak RK & Goldstein JL (1995). cDNA cloning of MCT2, a second monocarboxylate transporter expressed in different cells than MCT1. J Biol Chem 270, 1843-1849.
16. Garcia CK, Goldstein JL, Pathak RK, Anderson RG & Brown MS (1994). Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates: implications for the Cori cycle. Cell 76, 865-873.
17. Gaster M, Poulsen P, Handberg A, Schroder HD & Beck-Nielsen H (2000). Direct evidence of fiber type-dependent GLUT-4 expression in human skeletal muscle. Am J Physiol Endocrinol Metab 278, E910-E916.
18. Halestrap AP & Price NT (1999). The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation. Biochem J 343, 281-299.
19. Hashimoto T, Kambara N, Nohara R, Yazawa M & Taguchi S (2004). Expression of MHC-beta and MCT1 in cardiac muscle after exercise training in myocardial-infarcted rats. J Appl Physiol 97, 843-851.
20. Jackson VN, Price NT, Carpenter L & Halestrap AP (1997). Cloning of the monocarboxylate transporter isoform MCT2 from rat testis provides evidence that expression in tissues is species-specific and may involve post-transcriptional regulation. Biochem J 324, 447-453.
21. Kern M, Wells JA, Stephens JM, Elton CW, Friedman JE, Tapscott EB, Pekala PH & Dohm GL (1990). Insulin responsiveness in skeletal muscle is determined by glucose transporter (Glut4) protein level. Biochem J 270, 397-400.
22. Lin RY, Vera JC, Chaganti RS & Golde DW (1998). Human monocarboxylate transporter 2 (MCT2) is a high affinity pyruvate transporter. J Biol Chem 273, 28959-28965.
23. McClelland GB & Brooks GA (2002). Changes in MCT 1, MCT 4, and LDH expression are tissue specific in rats after long-term hypobaric hypoxia. J Appl Physiol 92, 1573-1584.
24. McClelland GB, Khanna S, Gonzalez GF, Butz CE & Brooks GA, (2003). Peroxisomal membrane monocarboxylate transporters: evidence for a redox shuttle system? Biochem Biophys Res Commun 304, 130-135.
25. Merezhinskaya N, Ogunwuyi SA, Mullick FG & Fishbein WN (2004). Presence and localization of three lactic acid transporters (MCT1-2, and -4) in separated human granulocytes, lymphocytes, and monocytes. J Histochem Cytochem 52, 1483-1493.
26. Miller BF, Lindinger MI, Fattor JA, Jacobs KA, Leblanc PJ, Duong M, Heigenhauser GJF & Brooks GA (2005). Hematological and acid-base changes in men during prolonged exercise with and without sodium-lactate infusion. J Appl Physiol 98, 856-865.
27. Peter JB, Barnard RJ, Edgerton VR, Gillespie CA & Stempel KE (1972). Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry 11, 2627-2633.
28. Pilegaard H, Domino K, Noland T, Juel C, Hellsten Y, Halestrap AP & Bangsbo J (1999a). Effect of high-intensity exercise training on lactate/H+ transport capacity in human skeletal muscle. Am J Physiol 276, E255-E261.
29. + transporter isoforms MCT1 and MCT4 in human skeletal muscle. Am J Physiol 276, E843-E848.
30. Roth DA & Brooks GA (1990a). Lactate and pyruvate transport is dominated by a pH gradient-sensitive carrier in rat skeletal muscle sarcolemmal vesicles. Arch Biochem Biophys 279, 386-394.
31. Roth DA & Brooks GA (1990b). Lactate transport is mediated by a membrane-bound carrier in rat skeletal muscle sarcolemmal vesicles. Arch Biochem Biophys 279, 377-385.
32. Taguchi S, Hata Y & Itoh K (1985). Enzymatic responses and adaptations to swimming training and hypobaric hypoxia in postnatal rats. Jpn J Physiol 35, 1023-1032.
33. Watt PW, Maclennan PA, Hundal HS, Kuret CM & Rennie MJ (1988). L(+)-lactate transport in perfused rat skeletal muscle: kinetic characteristics and sensitivity to pH and transport inhibitors. Biochim Biophys Acta 944, 213-222.
34. Wattenberg LW & Leong JL (1960). Effects of coenzyme Q10 and menadione on succinic dehydrogenase activity as measured by tetrazolium salt reduction. J Histochem Cytochem 8, 296-303.
35. Wilson MC, Jackson VN, Heddle C, Price NT, Pilegaard H, Juel C, Bonen A, Montgomery I, Hutter OF & Halestrap AP (1998). Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3. J Biol Chem 273, 15920-15926.