Phenotypic and molecular characterization of Candida dubliniensis isolates from clinical specimens in Kuwait

Medical Principles and Practice

Volumn 14, Issue SUPPL. 1, 2005, Pages 77-83

  Ahmad, Suhail ^a   Mokaddas, Eiman ^a   Al Sweih, Noura ^a   Khan, Zia U ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Candida albicans; Candida dubliniensis; Isolation; Molecular characterization; rDNA sequencing

Indexed keywords

ALPHA METHYLGLUCOSIDE; INTERNAL TRANSCRIBED SPACER; LACTIC ACID; RIBOSOME DNA; TREHALOSE; XYLOSE;

CANDIDA ALBICANS; CANDIDA DUBLINIENSIS; CONFERENCE PAPER; CONTROLLED STUDY; DNA SEQUENCE; FUNGUS ISOLATION; GENE AMPLIFICATION; HUMAN IMMUNODEFICIENCY VIRUS; IMMUNE DEFICIENCY; MOLECULAR TYPING; MORPHOLOGICAL TRAIT; NONHUMAN; PLANT SEED; POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; SUNFLOWER;

ANTIFUNGAL AGENTS; CANDIDA; CANDIDIASIS; ELECTROPHORESIS, AGAR GEL; HUMANS; KUWAIT; MICROBIAL SENSITIVITY TESTS; MYCOLOGICAL TYPING TECHNIQUES; PHENOTYPE; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE;

EID: 23844472260     PISSN: 10117571     EISSN: None     Source Type: Journal    
DOI: 10.1159/000086188     Document Type: Conference Paper

References (39)

1. Sullivan D, Coleman DC: Candida dubliniensis: Characteristics and identification. J Clin Microbiol 1998;36:329-334.
2. Sullivan DJ, Moran GP, Pinjon E, Al-Mosaid A, Stokes C, Vaughan C, Coleman DC: Comparison of the epidemiology, drug resistance mechanisms, and virulence of Candida dubliniensis and Candida albicans. FEMS Yeast Res 2004;4:369-376.
3. Odds FC, Van Nuffel L, Dams G: Prevalence of Candida dubliniensis isolates in a yeast stock collection. J Clin Microbiol 1998;36:2869-2873.
4. Jabra-Rizk MA, Falkler WA Jr, Merz WG, Baqui AA, Kelly JI, Meiller TF: Retrospective identification and characterization of Candida dubliniensis isolates among Candida albicans clinical laboratory isolates from human immunodeficiency virus (HIV)-infected and non-HIV-infected individuals. J Clin Microbiol 2000;38:2423-2426.
5. Moran GP, Sullivan DJ, Henman MC, McCreary CE, Harrington B, Shanley DB, Coleman DC: Antifungal drug susceptibilities of oral Candida dubliniensis isolates from human immunodeficiency virus (HIV)-infected and non-HIV-infected subjects and generation of stable fluconazole-resistant derivatives in vitro. Antimicrob Agents Chemother 1997;41:617-623.
6. Brandt ME, Harrison LH, Pass M, Sofair AN, Huie S, Li R-K, Morrison CJ, Warnock DW, Hajjeh RA: Candida dubliniensis fungemia: The first four cases in North America. Emerg Infect Dis 2000;6:46-49.
7. Meis JPGM, Ruhnke M, DePauw BE, Odds FC, Siegert W, Verweij PE: Candida dubliniensis candidemia in patients with chemotherapy-induced neutropenia and bone marrow transplantation. Emerg Infect Dis 1999;5:150-153.
8. Sebti A, Kiehn TE, Perlin D, Chaturvedi V, Wong M, Doney A, Park S, Sepkowitz KA: Candida dubliniensis at a cancer center. Clin Infect Dis 2001;32:1034-1038.
9. Coleman DC, Rinaldi MG, Haynes KA, Rex JH, Summerbell RC, Annaissie EA, Li A, Sullivan DJ: Importance of Candida species other than Candida albicans as opportunistic pathogen. Med Mycol 1998;36(suppl 1):156-165.
10. Khan ZU, Ahmad S, Mokaddas E, Chandy R: Simplified sunflower (Halianthus annuus) seed agar for differentiation of Candida dubliniensis from Candida albicans. Clin Microbiol Infect 2004;10:590-592.
11. Staib P, Morschhauser J: Chlamydospore formation on Staib agar as a species-specific characteristic of Candida dubliniensis. Mycoses 1999;42:521-524.
12. Al Mosaid A, Sullivan DJ, Coleman DC: Differentiation of Candida dubliniensis from Candida albicans on Pal's agar. J Clin Microbiol 2003;4:4787-4789.
13. Khan ZU, Ahmad S, Mokaddas E, Chandy R: Tobacco agar: A new medium for differentiation of Candida dubliniensis from Candida albicans. J Clin Microbiol 2004;42;4796-4798.
14. Mosca CO, Moragues MD, Llovo J, Al Mosaid A, Coleman DC, Ponton J: Casein agar: A useful medium for differentiating Candida dubliniensis from Candida albicans. J Clin Microbiol 2003;41:1259-1262.
15. Kirkpatrick WR, Revankar SG, Mcatee RK, Lopez-Ribot JL, Fothergill AW, McCarthy DI, Sanche SE, Cantu RA, Rinaldi MG, Patterson TF: Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patients in North America by primary CHROMagar Candida screening and susceptibility testing of isolates. J Clin Microbiol 1998;36:3007-3012.
16. Donnelly SM, Sullivan DJ, Shanley DB, Coleman DC: Phylogenetic analysis and rapid identification of Candida dubliniensis based on analysis of ACT1 intron and exon sequences. Microbiology 1999;145:1871-1882.
17. Kurzai O, Heinz WJ, Sullivan DJ, Coleman DC, Frosch M, Muhlschlegel FA: Rapid PCR test for discriminating between Candida albicans and Candida dubliniensis isolates using primers derived from the pH regulated PHR1 and PHR2 genes of C. albicans. J Clin Microbiol 1999;37:1587-1590.
18. Ahmad S, Khan Z, Mustafa AS, Khan ZU: Seminested PCR for diagnosis of candidemia: Comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol 2002;40:2483-2489.
19. Ahmad S, Mustafa AS, Khan Z, Al-Rifaiy AI, Khan ZU: PCR-enzyme immunoassay of rDNA in the diagnosis of candidemia and comparison with amplicon detection by agarose gel electrophoresis. Int J Med Microbiol 2004;294:45-51.
20. Ahmad S, Khan Z, Mokaddas E, Khan ZU: Isolation and molecular identification of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Kuwait. J Med Microbiol 2004;53:633-637.
21. McGinnis MR: Mycology; in Isenberg HD (ed): Clinical Microbiology Procedures Handbook. Washington, American Society for Microbiology Press. 1994, section 6, pp 6.1.1.-6.1.12.
22. Alves SH, Milan EP, de Laet Sant'Ana P, Oliveira LO, Santurio JM, Colombo AL: Hypertonic sabouraud broth as a simple and powerful test for Candida dubliniensis screening. Diagn Mirobiol Infect Dis 2002;43:85-86.
23. Ahmad S, Mokaddas E, Al-Anoud J: Rapid detection of ethambutol-resistant Mycobacterium tuberculosis strains by PCR-RFLP targeting embB codons 306 and 497 and iniA 501 mutations. Mol Cell Probes 2004;18:299-306.
24. National Committee for Clinical Laboratory Standards: Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeast, Approved Standard M27-A. Wayne, National Committee for Clinical Laboratory Standards, 1997.
25. Pfaller MA, Messer SA, Karlsson A, Bolmstrom A: Evaluation of the Etest method for determining fluconazole susceptibilities of 402 clinical yeast isolates by using three different agar media. J Clin Microbiol 1998;36:2586-2589.
26. Pfaller MA, Messer SA, Houston A, Mills K, Bolmstrom A, Jones RN: Evaluation of the Etest method for determining voriconazole susceptibilities of 312 clinical isolates of Candida species by using three different agar media. J Clin Microbiol 2000;38:3715-3717.
27. Gales AC, Pfaller MA, Houston AK, Jolly SDJ, Sullivan DJ, Coleman DC, Soll DR: Identification of Candida dubliniensis based on temperature and utilization of xylose and alpha-methyl-D-glucoside as determined with the API 20C AUX and Vitek YBC Systems. J Clin Microbiol 1999;37:3804-3808.
28. Sancak B, Rex JH, Paetznick V, Chen E, Rodriguez J: Evaluation of a method for identification of Candida dubliniensis bloodstream isolates. J Clin Microbiol 2003;41:489-491.
29. Pincus DH, Coleman DC, Pruitt WR, Padhye AA, Salkin IF, Geimer M, Bassel A, Sullivan DJ, Clarke M, Hearn V: Rapid identification of Candida dubliniensis with commercial yeast identification systems. J Clin Microbiol 1999;37:3533-3539.
30. Kurzai O, Korting HC, Harmsen D, Bautsch W, Molitor M, Frosch M, Muhlschlegel FA: Molecular and phenotypic identification of the yeast pathogen Candida dubliniensis. J Mol Med 2000;78:521-529.
31. Tintelnot K, Haase G, Seibold M, Bergmann F, Staemmler M, Franz T, Naumann D: Evaluation of phenotypic markers for selection and identification of Candida dubliniensis. J Clin Microbiol 2000;38:1599-1608.
32. Gutierrez J, Morales P, Gonzalez MA, Quindos G: Candida dubliniensis, a new fungal pathogen. J Basic Microbiol 2002;42:207-227.
33. Al-Hedaithy SS: The yeast species causing fungemia at a university hospital in Riyadh, Saudi Arabia, during a 10-year period. Mycoses 2003;46:293-298.
34. Polacheck I, Strahilevitz J, Sullivan D, Donnelly S, Salkin IF, Coleman DC: Recovery of Candida dubliniensis from non-human immunodeficiency virus-infected patients in Israel. J Clin Microbiol 2000;38:170-174.
35. Ponton J, Ruchel R, Clemons KV, Coleman DC, Grillot R, Guarro J, Aldebert D, Ambroise-Thomas P, Cano J, Carrillo-Munoz AJ, Gene J, Pinel C, Stevens DA, Sullivan DJ: Emerging pathogens. Med Mycol 2000;38 (suppl 1):225-236.
36. Lefler E, McCullough MJ, Clemons KV, Stevens DA: Initial isolation of Candida dubliniensis from the Middle East. Int J Infect Dis 2001;5:40-42.
37. Fotedar R, Al Hedaithy SS: Prevalence of Candida dubliniensis among germ tube-positive yeasts recovered from the respiratory specimens in HIV-negative patients. Mycoses 2004;47:50-155.
38. Fotedar R, Al-Hedaithy SS: Candida dubliniensis at a university hospital in Saudi Arabia. J Clin Microbiol 2003;41:1907-1911.
39. Quindos G, Carrillo-Munoz AJ, Arevalo MP, Salgado J, Alonso-Vargas R, Rodrigo JM, Ruesga MT, Valverde A, Peman J, Canton E, Martin-Mazuelos E, Ponton J: In vitro susceptibility of Candida dubliniensis to current and new antifungal agents. Chemotherapy 2000;46:395-401.