Rapid PCR test for discriminating between Candida albicans and Candida dubliniensis isolates using primers derived from the pH-regulated PHR1 and PHR2 genes of C. albicans

Journal of Clinical Microbiology

Volumn 37, Issue 5, 1999, Pages 1587-1590

  Kurzai, Oliver ^a   Heinz, Werner J ^a   Sullivan, Derek J ^b   Coleman, David C ^b   Frosch, Matthias ^a   Mühlschlegel, Fritz A ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; OLIGONUCLEOTIDE; PRIMER DNA;

ARTICLE; CANDIDA ALBICANS; CANDIDA DUBLINIENSIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA DETERMINATION; FUNGAL GENETICS; FUNGUS IDENTIFICATION; FUNGUS SPORE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE HOMOLOGY; SPECIES DIFFERENTIATION;

CANDIDA ALBICANS; CANDIDA DUBLINIENSIS; FUNGI;

EID: 0032944885     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.37.5.1587-1590.1999     Document Type: Article

References (22)

1. Coleman, D. C., D. J. Sullivan, D. E. Bennett, G. P. Moran, H. J. Barry, and D. B. Shanley. 1997. Candidiasis: the emergence of a novel species, Candida dubliniensis. AIDS 11:557-567.
2. De Bernardis, F., F. A. Mühlschlegel, A. Cassone, and W. A. Fonzi. 1998. The pH of the host niche controls gene expression in and virulence of Candida albicans. Infect. Immun. 66:3317-3325.
3. Gallagher, P. J., D. E. Bennett, M. C. Henman, R. J. Russel, S. R. Flint, D. B. Shanley, and D. C. Coleman. 1992. Reduced azole susceptibility of Candida albicans from HIV-positive patients and a derivative exhibiting colony morphology variation. J. Gen. Microbiol. 138:1901-1911.
4. Gilfillan, G. D., D. J. Sullivan, K. Haynes, T. Parkinson, D. C. Coleman, and N. A. R. Gow. 1998. Candida dubliniensis: phylogeny and putative virulence factors. Microbiology 144:829-838.
5. Gillum, A. M., E. Y. H. Tsay, and D. R. Kirsch. 1984. Isolation of the Candida albicans gene for orotidine-5′-phosphate decarboxylase by complementation of S. cerevisiae ura3 and E. coli pyrF mutations. Mol. Gen. Genet. 198:179-182.
6. Langford, C. J., and D. Gallwitz. 1983. Evidence for an intron-containing sequence required for the splicing of yeast RNA polymerase II transcripts. Cell 33:519-527.
7. Moran, G. P., D. J. Sullivan, M. C. Henman, C. E. McCreary, B. J. Harrington, D. B. Shanley, and D. C. Coleman. 1997. Antifungal drug susceptibilities of oral Candida dubliniensis isolates from human immunodeficiency virus (HIV)-infected and non-HIV-infected subjects and generation of stable fluconazole-resistant derivatives in vitro. Antimicrob. Agents Chemother. 41:617-623.
8. Mühlschlegel, F. A., and W. A. Fonzi. 1997. PHR2 of Candida albicans encodes a functional homolog of the pH-regulated gene PHRI with an inverted pattern of pH-dependent expression. Mol. Cell. Biol. 17:5960-5967.
9. Nakazawa, T., H. Horiuchi, A. Ohta, and M. Takagi. 1998. Isolation and characterization of EPD1, an essential gene for pseudohyphal growth of a dimorphic yeast, Candida maltosa. J. Bacteriol. 180:2079-2086.
10. Odds, F. C. 1974. The effect of growth medium on filament production in Candida albicans. Sabouraudia 12:112-119.
11. Odds, F. C. 1988. Biological aspects of pathogenic Candida species, p. 7-15. In F. C. Odds (ed.), Candida and candidosis. Bailliere Tindall, London, United Kingdom.
12. Pinjon, E., D. Sullivan, I. Salkin, D. Shanley, and D. Coleman. 1998. Simple, inexpensive, reliable method for differentiation of Candida dubliniensis from Candida albicans. J. Clin. Microbiol. 36:2093-2095.
13. Reef, S. E., and K. H. Meyer. 1995. Opportunistic candidal infections in patients infected with human immunodeficiency virus: prevention issues and priorities. Clin. Infect. Dis. 21(Suppl. 1):S99-S102.
14. Salkin, I. F., W. R. Pruitt, A. A. Padhye, D. Sullivan, D. Coleman, and D. H. Pincus. 1998. Distinctive carbohydrate assimilation profiles used to identify the first clinical isolates of Candida dubliniensis recovered in the United States. J. Clin. Microbiol. 36:1467. (Letter.)
15. Saporito-Irwin, S. M., C. E. Birse, P. S. Sypherd, and W. A. Fonzi. 1995. PHRI, a pH-regulated gene of Candida albicans, is required for morphogenesis. Mol. Cell. Biol. 15:601-613.
16. Scherer, S., and D. S. Stevens. 1988. A Candida albicans dispersed, repeated gene family and its epidemiological applications. Proc. Natl. Acad. Sci. USA 85:1452-1456.
17. Schoofs, A., F. C. Odds, R. Colebunders, M. Ieven, and H. Goossens. 1997. Use of specialised isolation media for recognition and identification of Candida dubliniensis isolates from HIV-infected patients. Eur. J. Clin. Microbiol. Infect. Dis. 16:296-300.
18. Sullivan, D., K. Haynes, J. Bille, P. Boerlin, L. Rodero, S. Lloyd, M. Henman, and D. Coleman. 1997. Widespread geographic distribution of oral Candida dubliniensis strains in human immunodeficiency virus-infected individuals. J. Clin. Microbiol. 35:960-964.
19. Sullivan, D., and D. Coleman. 1998. Candida dubliniensis: characteristics and identification. J. Clin. Microbiol. 36:329-334.
20. Sullivan, D. J., T. J. Westerneng, K. A. Haynes, D. E. Bennett, and D. C. Coleman. 1995. Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiology 141:1507-1521.
21. Vai, M., E. Gatti, E. Lacana, L. Popolo, and L. Alberghina. 1991. Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region. J. Biol. Chem. 266:12242-12248.
22. Weig, M., and F. Mühlschlegel. Unpublished data.