Molecular study on cloned endoglucanase gene from rumen bacterium

Journal of Molecular Microbiology and Biotechnology

Volumn 8, Issue 2, 2005, Pages 111-116

  Ozkose, Emin ^a   Akyol, Ismail ^a   Ekinci, Mehmet Sait ^a  

^a KAHRAMANMARAS SUTCU IMAM UNIVERSITY   (Turkey)

Author keywords

Cloning; Endoglucanase gene; Expression; Rumen bacteria; Stability

Indexed keywords

AMYLASE; GLUCAN SYNTHASE; CELLULASE; DEOXYRIBONUCLEASE; RECOMBINANT PROTEIN;

ANAEROBIC BACTERIUM; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL SURVIVAL; CELL ACTIVITY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; GENE INSERTION SEQUENCE; GENETIC STABILITY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PROMOTER REGION; RUMINOCOCCUS FLAVEFACIENS; SHUTTLE VECTOR; STREPTOCOCCUS BOVIS; ANIMAL; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; GRAM POSITIVE COCCI; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PLASMID; RUMINANT STOMACH;

ESCHERICHIA COLI; RUMINOCOCCUS FLAVEFACIENS; STREPTOCOCCUS BOVIS;

ANIMALS; CELLULASE; CLONING, MOLECULAR; ENDODEOXYRIBONUCLEASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GRAM-POSITIVE COCCI; PLASMIDS; RECOMBINANT PROTEINS; RUMEN;

EID: 23844437011     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000084566     Document Type: Article

References (26)

1. Bhat MK: Cellulases and related enzymes in biotechnology. Biotechnol Advan 2000;18:355-383.
2. Cunningham C, McPherson CA, Martin J, Harris WJ, Flint HJ: Sequence of a cellulase gene from the rumen anaerobe Ruminococcus flavefaciens 17. Mol Gen Genet 1991;228:320-323.
3. Ekinci MS, Flint HJ: Expression of bifunctional genes encoding xylanase and β(1,3-1,4)-glucanase in gram-positive bacteria. Turk J Vet Anim Sci 2001;25:771-775.
4. Ekinci MS, Flint HJ: Transformation and stability of cloned polysaccharidase genes in Gram-positive bacteria. Turk J Vet Anim Sci 2002;26:303-308.
5. Ekinci MS, Martin JC, Flint HJ: Streptococcus bovis as a host for the expression of cloned polysaccharidase genes. Biotechnol Lett 2001;23:501-505.
6. Ekinci MS, Martin JC, Flint HJ: Expression of cellulase gene, celA, from the rumen fungus Neocallimastix patriciarum in Streptococcus bovis by means of promoter fusions. Biotechnol Lett 2002;24:735-741.
7. Flint HJ: Molecular genetics of obligate anaerobes from the rumen. FEMS Microbiol Lett 1994;121:259-268.
8. Flint HJ, Forsberg CW: Polysaccharide degradation in the rumen: Biochemistry and genetics; in Engelhardt WV, Leonhard-Marek S, Breues G, Geiesecke D (eds): Ruminant Physiology: Digestion, Metabolism, Growth and Reproduction: Proceedings of the Eighth International Symposium on Ruminant Physiology. Stuttgart, Enke, 1995, pp 43-66.
9. Flint HJ, Martin J, McPherson CA, Daniel AS, Zhang JXA: Bifunctional enzyme, with separate xylanase and β(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. J Bacteriol 1993;175:2943-2951.
10. Forano E, Flint HJ: Genetically modified organisms: Consequences for ruminant health and nutrition. Ann Zootech 2000;49:255-271.
11. Gilkes NR, Henrissat B, Kilburn DG, Miller RC Jr, Warren RAJ: Domains in microbial β-1,4-glycanases: Sequence conservation, function and enzyme families. Microbiol Rev 1991;55:303-315.
12. Green J, Scott C, Guest JR: Functional versatility in the CRP-FNR superfamily of transcription factors: FNR and FLP. Adv Microb Physiol 2001;44:1-34.
13. Hanahan D: Techniques for transformation of Escherichia coli; in Glover DM (ed): DNA Cloning: A Practical Approach. Oxford, IRL Press, 1985, vol 1, pp 121-127.
14. Hobson PN: Rumen bacteria; in Norris JR, Ribbons DW (eds): Methods in Microbiology 3B. London, Academic Press, 1969, pp 133-149.
15. Lever M: Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): Effect of bismuth on the reaction. Anal Biochem 1977;81:21-27.
16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with Folin phenol reagent. J Biol Chem 1951;193:265-275.
17. Maniatis T, Fritsch EF, Sambrook J: Molecular cloning: A laboratory manual. Cold Spring Harbour, Cold Spring Harbor Laboratory, 1982.
18. Mercer DK, Melville CM, Scott KP, Flint HJ: Natural genetic transformation in the rumen bacterium Streptococcus bovis JB1. FEMS Microbiol Lett 1999;179:485-490.
19. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, ed 2. Cold Spring Harbor, Cold Spring Harbor Laboratory, 1989.
20. Teather RM: Structure of β-glucanase genes from rumen bacteria; in Akin DE, Ljungdahl LG, Wilson JR, Harris PJ (eds): Microbial and Plant Opportunities to Improve Lignocellulose Utilisation by Ruminants. New York, Elsevier Science, 1990, pp 377-388.
21. Teather RM, Wood PJ: Use of Congo red-polysaccharide interactions in enumeration and characterisation of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 1982;43:777-780.
22. Vercoe PE, Gregg K: DNA sequence and transcription of an endoglucanase gene from Bacteroides (Prevotella) ruminicola AR20. Mol Gen Genet 1992;233:84-292.
23. Vercoe PE, Gregg K: Sequence and transcriptional analysis of an endoglucanase from Ruminococcus albus AR67. Anim Biotechnol 1995;6:59-71.
24. Whitehead TR: Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria. Curr Microbiol 1993;27:27-34.
25. Whitehead TR, Flint HJ: Heterologous expression of an endoglucanase gene (endA) from the ruminal anaerobe Ruminococcus flavefaciens 17 in Streptococcus bovis and Streptococcus sanguis. FEMS Microbiol Lett 1995;126:165-170.
26. Wyckoff HA, Whitehead TR: Improved electroporation protocol and vectors for Streptococcus bovis. World J Microbiol Biotechnol 1997;13:269-272.