Expression of a cellulase gene, celA, from the rumen fungus Neocallimastix patriciarum in Streptococcus bovis by means of promoter fusions

Biotechnology Letters

Volumn 24, Issue 9, 2002, Pages 735-741

  Ekinci, Mehmet Sait ^a   Martin, Jennifer C ^b   Flint, Harry J ^b  

^a KAHRAMANMARAS SUTCU IMAM UNIVERSITY   (Turkey)

^b ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Cellulase; Neocallimastix patriciarum; Promoter fusion; Streptococcus bovis JB1

Indexed keywords

CELLULASE; GLUCAN GLUCOSIDASE; SIGNAL PEPTIDE;

ARTICLE; CELA GENE; EUKARYOTE; FUNGAL GENE; FUNGUS; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE FUSION; GENETIC TRANSFORMATION; NEOCALLIMASTIX PATRICIARUM; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; RUMEN BACTERIA; RUMINOCOCCUS FLAVEFACIENS; SIGNAL TRANSDUCTION; STREPTOCOCCUS BOVIS;

BACTERIA (MICROORGANISMS); EUKARYOTA; FUNGI; NEOCALLIMASTIX; NEOCALLIMASTIX PATRICIARUM; POSIBACTERIA; PROKARYOTA; RUMINOCOCCUS; RUMINOCOCCUS FLAVEFACIENS; STREPTOCOCCUS; STREPTOCOCCUS BOVIS; UNIDENTIFIED SHUTTLE VECTOR;

EID: 0036233418     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1015250504093     Document Type: Article

References (16)

1. Gene knockout of the intracellular amylase gene by homologous recombination in Streptococcus bovis
2. Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Tricoderma reesei cellobiohydrolase II
3. Expression of bifunctional genes encoding xylanase and β(1,3-1,4)-glucanase in gram-positive bacteria
4. Streptococcus bovis as a host for the expression of cloned polysaccharidase genes
5. Isolation and overexpression of a gene encoding an extracellular β-(1,3-1,4)-glucanase from Streptococcus bovis JB1
6. A bifunctional enzyme, with separate xylanase and β(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens
7. Selection and application of Streptococcus bovis as a silage inoculant
8. Natural genetic transformation of in the rumen bacterium Streptococcus bovis JB1
9. Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH
10. The green fluorescent protein as a visible marker for lactic acid bacteria in complex anaerobic ecosystems
11. The rumen: A unique source of enzymes for enhancing livestock production
12. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis
13. Heterologous expression of an endoglucanase gene (endA) from the ruminal anaerobe Ruminococcus flavefaciens in Streptococcus bovis and Streptococcus sanguis
14. Improved electroporation protocol and vectors for Streptococcus bovis
15. Cloning and expression of multiple cellulase cDNAs from the anaerobic fungus Neocallimastix patriciarum in Escherichia coli
16. Comparison of the influence of carbon substrates on the fibrolytic activities of anaerobic rumen fungi