Expression of Bifunctional Genes Encoding Xylanase and β(1,3-1,4)-Glucanase in Gram-Positive Bacteria

Turkish Journal of Veterinary and Animal Sciences

Volumn 25, Issue 5, 2001, Pages 771-775

  Ekinci, Mehmet Sait ^a   Flint, Harry J ^b  

^a KAHRAMANMARAS SUTCU IMAM UNIVERSITY   (Turkey)

^b ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Enzyme; Gene expression; Gram positive bacteria

Indexed keywords

EID: 0035601767     PISSN: 13000128     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Herbers, K., Flint, H. J., and Sonnewald, V. Apoplastic expression of the xylanase and β(1,3-1,4) glucanase domains of the xynD gene from Ruminococcus flavefaciens leads to functional polypeptides in transgenic tobacco plants. Molecular Breeding. 1996; 2: 81-87.
2. Van Rensburg, P., van Zyl, W. H, and Pretorius, I. S. Expression of the Ruminococcus flavefaciens cellodextrian gene in Streptococcus cerevisii. Biotechnol. Letts. 1995; 17: 481-486.
3. Ekinci, M. S., Karaman, M., and Efe, E. Biotechnology, application of possible genetic strategies for poultry and ruminant nutrition. International Animal Nutrition Congress "2000" 4-6 September, Isparta/Turkey, 2000; 294-299.
4. Whitehead, T. R. Flint, H. J. Heterologous expression of an endoglucanase gene (endA) from the ruminai anaerobe Ruminococcus flavefaciens 17 in Streptococcus bovis and Streptococcus sanguis. FEMS Microbiol Lett. 1995; 126: 165-170
5. Jones, B. A., Mucke, R. F., and Ricke S. C. Selection and application of Streptococcus bovis as a silage inoculant. Appl. Environ. Microbiol. 1991; 57: 3000-3005.
6. Satoh, E., Ho, Y., Sasaki, Y., and Sasaki, T. Application of the extracellular a-amylase gene from Streptococcus bovis 148 to construction of a separation water for yogurt starter strains. Appl. Environ. Microbiol. 1997; 63: 4593-4596.
7. Ekinci, M.S., McCrae, S.I., and Flint, H.J. Isolation and overexpression of a gene encoding an extracellular β-(1,3-1,4)-glucanase from Streptococcus bovis JB1. Appl. Environ. Microbiol. 1997; 63: 3762-3756.
8. Hespell, R. B., and Whitehead, T. R. Introduction of Tn916 and pAMβ1 into Streptococcus bovis JB1 by conjungation. Appl. Environ. Microbiol. 1991; 57: 2710-2713.
9. nd Joint INRA-RRI gastrointestinal tract Microbiology symposium, Clermont-Ferrand, France, in "Reproduction Nutrition Development. 2000; 40: 2217.
10. Mercer, D. K., Melville, C. M., Scott, K. P., and Flint, H. J. Natural genetic transformation in the rumen bacterium Streptococcus bovis JB1. FEMS Microbiol. Letts. 1999; 179: 485-490.
11. Macrina, F. L., Tobian, J. A., Jones, K. R. and Evans, R. P. Clewell D. B. A cloning vector able to replicate in Escherichia coli and Streptococcus sanguis. Gene. 1982; 19: 345-353.
12. Simon, D., and Chopin, A. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Biochimie. 1988; 70: 559-566.
13. Hobson, P. N. Rumen bacteria. In "Methods in microbiology" 3B ed Norris JR, Ribbons DW, London. Academic Press, 1969; 133-149.
14. Whitehead, T. R. Analyses of the gene and amino acid sequence of the Prevotella (Bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria. Curr. Microbiol. 1993; 27: 27-34.
15. Holo, H., and Nes, I. F. High frequency transformation, by electroporation, of Lactococcus lactis subsp. Cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 1989; 55: 3119-3123.
16. Cruz-Rodz, A. L. and Gilmore, M. S. Electroporation of glycine-treated Enterococcus faecalis, In "Genetics and Molecular Biology of Streptococci. Lactococci and Enterococci" Eds. Dunny, G.M., Cleary, P.P., McKay, L.L. ASM Washington, D.C. 1991; 300.
17. Lever, M. Carbohydrate determination with 4-hydroxybenzoic acid hydrazide (PAHBAH): effect of bismuth on the reaction. Anal. Biochem. 1977; 81: 21-27.
18. Teather, R. M., and Wood, P. J. Use of Congo Red-polysaccharide interactions in enumeration and characterisation of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 1982; 43: 777-780
19. Flint, H. J. Martin, J., McPherson, C. A., Daniel, A. S., and Zhang, J-X. A bifunctional enzyme, with separate xylanase and β(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. J. Bacteriol. 1993; 175: 2943-2951.
20. Kirby, J., Martin, J. C., Daniel, A. S., and Flint, H. J. Dockerin-like sequences in cellulases and xylanases from the rumen cellulolytic bacterium Ruminococcus fiavefaciens. FEMS Microbiol. Lett. 1997; 149: 213-219.
21. Scott, K. P. Mercer, D. K. Glover, L. A. and Flint H. J. The green fluorescent protein as a visible marker for lactic acid bacteria in complex anaerobic ecosystems. FEMS Microbiol. Ecol. 1998; 26: 219-230.
22. Deguchi, H., Watanabe Y., Sasaki T., Matsuda T., Shimizu S., and Ohmiya K. Purification and properties of the endo-1,4-β-glucanase from Rumnococcus albus and its gene product in Escherichia coli. J. Ferment. Bioeng. 1991; 71: 221-225.
23. Erickson, P. R., and Herzberg, M. C. Evidence for the covalent linkage of carbohydrate polymers to a glycoprotein from Streptococcussanguis. J. Biol. Chem. 1993; 268: 23780-23783.