Postmitotic nuclear retention of episomal plasmids Is altered by DNA labeling and detection methods

Molecular Therapy

Volumn 12, Issue 3, 2005, Pages 460-467

  Gasiorowski, Joshua Z ^a   Dean, David A ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Episomes; Fluorophores; Gene therapy; Microinjection; Mitosis; Nuclear import; Peptide nucleic acid; Plasmids; Transfection

Indexed keywords

PEPTIDE NUCLEIC ACID; PLASMID DNA;

ARTICLE; CELL NUCLEUS; CELLULAR DISTRIBUTION; DNA TRANSFECTION; EPISOME; GENE TARGETING; HELA CELL; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; LABORATORY; MITOSIS; NONVIRAL GENE THERAPY; NORMAL DISTRIBUTION; NUCLEAR IMPORT; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; STATISTICAL SIGNIFICANCE;

BIOLOGICAL TRANSPORT; CELL DIVISION; CELL NUCLEUS; DNA; FLUORESCENT DYES; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENETIC TECHNIQUES; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION; MITOSIS; MODELS, BIOLOGICAL; NORMAL DISTRIBUTION; PLASMIDS; TRANSFECTION;

EID: 23844435112     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymthe.2005.05.001     Document Type: Article

References (33)

1. H. Herweijer G. Zhang V.M. Subbotin V. Budker P. Williams and J.A. Wolff Time course of gene expression after plasmid DNA gene transfer to the liver J. Gene Med. 3 2001 280-291
2. J.A. Wolff J.J. Ludtke G. Acsadi P. Williams and A. Jani Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle Hum. Mol. Genet. 1 1992 363-369
3. T. Muramatsu et al. In vivo gene electroporation in skeletal muscle with special reference to the duration of gene expression Int. J. Mol. Med. 7 2001 37-42
4. J.B. Martin J.L. Young J.N. Benoit and D.A. Dean Gene transfer to intact mesenteric arteries by electroporation J. Vasc. Res. 37 2000 372-380
5. K. Blair-Parks B.C. Weston and D.A. Dean High-level gene transfer to the cornea using electroporation J. Gene Med. 4 2002 92-100
6. S. Li et al Increased level and duration of expression in muscle by co-expression of a transactivator using plasmid systems Gene Ther. 6 1999 2005-2011
7. D.A. Dean B.S. Dean S. Muller and L.C. Smith Sequence requirements for plasmid nuclear import Exp. Cell Res. 253 1999 713-722
8. J.J. Ludtke M.G. Sebestyen and J.A. Wolff The effect of cell division on the cellular dynamics of microinjected DNA and dextran Mol. Ther. 5 2002 579-588
9. C.M. Feldherr and D. Akin The permeability of the nuclear envelope in dividing and nondividing cell cultures J. Cell Biol. 111 1990 1-8
10. W.C. Tseng F.R. Haselton and T.D. Giorgio Mitosis enhances transgene expression of plasmid delivered by cationic liposomes Biochim. Biophys. Acta 1445 1999 53-64
11. V. Escriou M. Carriere F. Bussone P. Wils and D. Scherman Critical assessment of the nuclear import of plasmid during cationic lipid-mediated gene transfer J. Gene Med. 3 2001 179-187
12. H. Akita R. Ito I.A. Khalil S. Futaki and H. Harashima Quantitative three-dimensional analysis of the intracellular trafficking of plasmid DNA transfected by a nonviral gene delivery system using confocal laser scanning microscopy Mol. Ther. 9 2004 443-451
13. O. Zelphati X. Liang P. Hobart and P.L. Felgner Gene chemistry: functionally and conformationally intact fluorescent plasmid DNA Hum. Gene Ther. 10 1999 15-24
14. G.A. Banks R.J. Roselli R. Chen and T.D. Giorgio A model for the analysis of nonviral gene therapy Gene Ther. 10 2003 1766-1775
15. R.P. van Gijlswijk et al. Universal Linkage System: Versatile nucleic acid labeling technique Expert Rev. Mol. Diagn. 1 2001 81-91
16. G.H. Keller D.P. Huang and M.M. Manak Labeling of DNA probes with a photoactivatable hapten Anal. Biochem. 177 1989 392-395
17. C. Neves G. Byk V. Escriou F. Bussone D. Scherman and P. Wils Novel method for covalent fluorescent labeling of plasmid DNA that maintains structural integrity of the plasmid Bioconjugate Chem. 11 2000 51-55
18. P.S. Slattum et al. Efficient in vitro and in vivo expression of covalently modified plasmid DNA Mol. Ther. 8 2003 255-263
19. A.C. Forster J.L. McInnes D.C. Skingle and R.H. Symons Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin Nucleic Acids Res. 13 1985 745-761
20. D.A. Dean Gene Delivery by Direct Injection and Facilitation of Expression by Mechanical Stretch 2005 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
21. M. Chalfie Y. Tu G. Euskirchen W.W. Ward and D.C. Prasher Green fluorescent protein as a marker for gene expression Science 263 1994 802-805
22. Zelphati, O. et al. (2000). PNA-dependent gene chemistry: Stable coupling of peptides and oligonucleotides to plasmid DNA. Biotechniques 28: 304-310, 312-314, 316.
23. V.V. Demidov et al. Stability of peptide nucleic acids in human serum and cellular extracts Biochem. Pharmacol. 48 1994 1310-1313
24. G.L. Wilson B.S. Dean G. Wang and D.A. Dean Nuclear import of plasmid DNA in digitonin-permeabilized cells requires both cytoplasmic factors and specific DNA sequences J. Biol. Chem. 274 1999 22025-22032
25. O. Zelphati et al. PNA-dependent gene chemistry: Stable coupling of peptides and oligonucleotides to plasmid DNA Biotechniques 28 2000 304-316
26. A.M. Belikova V.F. Zarytova and N.I. Grineva Synthesis of ribonucleosides and diribonucleoside phosphates containing 2-chloroethylamine and nitrogen mustard residues Tetrahedron Lett. 37 1967 3557-3562
27. D.A. Dean Import of plasmid DNA into the nucleus is sequence specific Exp. Cell Res. 230 1997 293-302
28. M.P. Calos Stability without a centromere Proc. Natl. Acad. Sci. USA 95 1998 4084-4085
29. J. Sears M. Ujihara S. Wong C. Ott J. Middeldorp and A. Aiyar The amino terminus of Epstein-Barr Virus (EBV) nuclear antigen 1 contains AT hooks that facilitate the replication and partitioning of latent EBV genomes by tethering them to cellular chromosomes J. Virol. 78 2004 11487-11505
30. J. Sears J. Kolman G.M. Wahl and A. Aiyar Metaphase chromosome tethering is necessary for the DNA synthesis and maintenance of oriP plasmids but is insufficient for transcription activation by Epstein-Barr nuclear antigen 1 J. Virol. 77 2003 11767-11780
31. P. Krieg E. Amtmann D. Jonas H. Fischer K. Zang and G. Sauer Episomal simian virus 40 genomes in human brain tumors Proc. Natl. Acad. Sci. USA 78 1981 6446-6450
32. D.D. Newmeyer J.M. Lucocq T.R. Burglin and E.M. De Robertis Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation EMBO J. 5 1986 501-510
33. J. Newport Nuclear reconstitution in vitro: Stages of assembly around protein-free DNA Cell 48 1987 205-217