A new mechanism for chloramphenicol, florfenicol and clindamycin resistance: Methylation of 23S ribosomal RNA at A2503

Molecular Microbiology

Volumn 57, Issue 4, 2005, Pages 1064-1073

  Kehrenberg, Corinna ^a   Schwarz, Stefan ^a   Jacobsen, Lene ^b   Hansen, Lykke H ^b   Vester, Birte ^b  

^a INSTITUT FÜR TIERZUCHT   (Germany)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORAMPHENICOL; CLINDAMYCIN; FLORFENICOL; RIBOSOME RNA; RNA DIRECTED DNA POLYMERASE; RNA METHYLTRANSFERASE;

ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; DNA FOOTPRINTING; DRUG BINDING; DRUG MECHANISM; DRUG RESISTANCE; DRUG TARGETING; ESCHERICHIA COLI; GENE TARGETING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RIBOSOME; STAPHYLOCOCCUS SCIURI; TANDEM MASS SPECTROMETRY;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; CHLORAMPHENICOL; CLINDAMYCIN; DRUG RESISTANCE, MICROBIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; METHYLATION; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; RIBOSOMES; RNA, RIBOSOMAL, 23S; STAPHYLOCOCCUS; THIAMPHENICOL;

ESCHERICHIA COLI; STAPHYLOCOCCUS; STAPHYLOCOCCUS SCIURI;

EID: 23744453392     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2005.04754.x     Document Type: Article

References (40)

1. Andersen, T.E., Porse, B.T., and Kirpekar, F. (2004) A novel partial modification at C2501 in Escherichia coli 23S ribosomal RNA. RNA 6: 907-913.
2. Arcangioli, M.A., Leroy-Setrin, S., Martel, J.L., and Chaslus-Dancla, E. (1999) A new chloramphenicol and florfenicol resistance gene linked to an integron structure in Salmonella typhimurium DT104. FEMS Microbiol Lett 174: 327-332.
3. Branlant, C., Krol, A., Machatt, M.A., Pouyet, J., Ebel, J.P., Edwards, K., and Kossel, H. (1981) Primary and secondary structures of Escherichia coli MRE 600, 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs. Nucleic Acids Res 9: 4303-4324.
4. Briggs, C.E., and Fratamico, P.M. (1999) Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104. Antimicrob Agents Chemother 43: 846-849.
5. Douthwaite, S. (1992) Interaction of the antibiotic clindamycin and lincomycin with Escherichia coli 23S ribosomal RNA. Nucleic Acids Res 20: 4717-4720.
6. Douthwaite, S., Fourmy, D., and Yoshizawa, S. (2005) Nucleotide methylations in rRNA that confer resistance to ribosome-targeting antibiotics. In Topics in Current Genetics 12: Fine-tuning of RNA Functions by Modification and Editing. Grosjean, H. (ed.). New York: Springer Verlag, pp. 287-309.
7. Gutell, R.R., Larsen, N., and Woese, C.R. (1994) Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol Rev 58: 10-26.
8. Hansen, J., Moore, P.B., and Steitz, T.A. (2003) Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit. J Mol Biol 289: 905-920.
9. Harms, J., Schluenzen, F., Zarivach, R., Bashan, A., Gat, S., Agmon, I., et al. (2001) High resolution structure of the large ribosomal subunit from a mesophilic eubacterium. Cell 107: 679-688.
10. Kehrenberg, C., and Schwarz, S. (2004) Identification of fexA, a novel gene from Staphylococcus lentus coding for resistance to florfenicol and chloramphenicol. Antimicrob Agents Chemother 48: 615-618.
11. Kehrenberg, C., Ojo, K.K., and Schwarz, S. (2004) Nucleotide sequence and organisation of the multiresistance plasmid pSCFS1 from Staphylococcus sciuri. J Antimicrob Chemother 54: 936-939.
12. Kirpekar, F., Douthwaite, S., and Roepstorff, P. (2000) Mapping posttranscriptional modifications in 5S ribosomal RNA by MALDI mass spectrometry. RNA 6: 296-306.
13. Kowalak, J.A., Bruenger, E., and McCloskey, J.A. (1995) Posttranscriptional modifications of the central loop of domain V in Escherichia coli 23 S ribosomal RNA. J Biol Chem 270: 17758-17764.
14. Lankat-Buttgereit, B., Gross, H.J., and Krupp, G. (1987) Detection of modified nucleosides by rapid RNA sequencing methods. Nucleic Acids Res 15: 7649.
15. Long, K.S., and Porse, B.T. (2003) A conserved chloramphenicol binding site at the entrance to the ribosomal peptide exit tunnel. Nucleic Acid Res 31: 7208-7215.
16. Maden, B.E. (1990) The numerous modified nucleotides in eukaryotic ribosomal RNA. Prog Nucleic Acid Res Mol Biol 39: 241-303.
17. McLuckey, S.A., Van Berkel, G.J., and Glish, G.L. (1992) Tandem mass spectrometry of small multiply charged oligonucleotides. J Am Soc Mass Spectrom 3: 60-70.
18. Maden, B.E., Corbett, M.E., Heeney, P.A., Pugh, K., and Ajuh, P.M. (1995) Classical and novel approaches to the detection and localization of the numerous modified nucleotides in eukaryotic ribosomal RNA. Biochimie 77: 22-29.
19. Mann, P.A., Xiong, L., Mankin, A.S., Chau, A.S., Mendrick, C.A., Najarian, D.J., et al. (2001) EmtA, a rRNA methyltransferase conferring high-level evernimicin resistance. Mol Microbiol 41: 1349-1356.
20. Mengel-Jørgensen, J., and Kirpekar, F. (2002) Detection of pseudouridine and other modifications in tRNA by cyanoethylation and MALDI mass spectrometry. Nucleic Acids Res 30: e135.
21. Moazed, D., and Noller, H.F. (1987) Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S rRNA. Biochimie 69: 879-884.
22. National Committee for Clinical Laboratory Standards (NCCLS) (2002) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standard. NCCLS document M31-A2. NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087, USA.
23. Nissen, P., Hansen, J., Ban, N., Moore, P.B., and Steitz, T.A. (2000) The structural basis of ribosome activity in peptide bond synthesis. Science 289: 920-930.
24. Poulsen, S.M., Karlsson, M., Johansson, L.B., and Vester, B. (2001) The pleuromutilin drugs tiamulin and valnemulin bind to the RNA at the peptidyl transferase centre on the ribosome. Mol Microbiol 41: 1091-1100.
25. Roberts, M.C., Sutcliffe, J., Courvalin, P., Jensen, L.B., Rood, J., and Seppala, H. (1999) Nomenclature for macrolide and macrolide-lincosamide- streptogramin B resistance determinants. Antimicrob Agents Chemother 43: 2823-2830.
26. Rodriguez-Fonseca, C., Amils, R., and Garrett, R.A. (1995) Fine structure of the peptidyl transferase centre on 23S-like rRNAs deduced from chemical probing of antibiotic-ribosome complexes. J Mol Biol 247: 224-235.
27. Schlünzen, F., Zarivach, R., Harms, J., Bashan, A., Tocilj, A., Albrecht, R., et al. (2001) Structural basis for the interaction of antibiotics with the peptidyl transferase centre in eubacteria. Nature 413: 814-821.
28. Schwarz, S., Cardoso, M., and Blobel, H. (1989) Plasmid-mediated chloramphenicol resistance in Staphylococcus hyicus. J Gen Microbiol 135: 3329-3336.
29. Schwarz, S., Werckenthin, C., and Kehrenberg, C. (2000) Identification of a plasmid-borne chloramphenicol/florfenicol resistance gene in Staphylococcus sciuri. Antimicrob Agents Chemother 44: 2530-2533.
30. Schwarz, S., Kehrenberg, C., and Ojo, K.K. (2002) Staphylococcus sciuri gene erm(33), encoding inducible resistance to macrolides, lincosamides and streptogramin B antibiotics, is a product of recombination between erm(C) and erm(A). Antimicrob Agents Chemother 46: 3621-3623.
31. Schwarz, S., Kehrenberg, C., Doublet, B., and Cloeckaert, A. (2004) Molecular basis of bacterial resistance to chloramphenicol and florfenicol. FEMS Microbiol Rev 28: 519-542.
32. Skinner, R., Cundliffe, E., and Schmidt, F.J. (1983) Site of action of a ribosomal RNA methylase responsible for resistance to erythromycin and other antibiotics. J Biol Chem 258: 12702-12706.
33. Sofia, H.J., Chen, G., Hetzler, B.G., Reyes-Spindola, J.F., and Miller, N.E. (2001) Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. Nucleic Acids Res 29: 1097-1106.
34. Treede, I., Jacobsen, L., Kirpekar, F., Vester, B., Weitnauer, G., Bechthold, A., and Douthwaite, S. (2003) The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose. Mol Microbiol 49: 309-318.
35. BK antibiotics bound to mutated large ribosomal subunits provide a structural explanation for resistance. Cell 121: 257-270.
36. Vasquez, D. (1979) Inhibitors of Protein Synthesis. Berlin: Springer-Verlag.
37. Vester, B., and Douthwaite, S. (2001) Macrolide resistance conferred by base substitutions in 23S rRNA. Antimicrob Agents Chemother 45: 1-12.
38. Vester, B., and Garrett, R.A. (1988) The importance of highly conserved nucleotides in the binding region of chloramphenicol at the peptidyl transferase centre of Escherichia coli 23S ribosomal RNA. EMBO J 7: 3577-3587.
39. Weisblum, B. (1995) Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother 39: 577-585.
40. Zalacain, M., and Cundliffe, E. (1989) Methylation of 23S rRNA caused by tlrA (ermSF), a tylosin resistance determinant from Streptomyces fradiae. J Bacteriol 171: 4254-4260.