Optimal conditions to use Pfu exo- DNA polymerase for highly efficient ligation-mediated polymerase chain reaction protocols

Nucleic Acids Research

Volumn 29, Issue 16, 2020, Pages

  Angers, Martin ^a,b   Cloutier, Jean François ^a,b   Castonguay, André ^b   Drouin, Régen ^a,b  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; GENOMIC DNA; BACTERIOPHAGE T7 INDUCED DNA POLYMERASE; DNA; DNA DIRECTED DNA POLYMERASE; INORGANIC SALT; PIPERIDINE; PIPERIDINE DERIVATIVE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PRIMER DNA; TAQ POLYMERASE;

ARTICLE; CONTROLLED STUDY; DNA DAMAGE; DNA SEQUENCE; DNA STRUCTURE; HUMAN; HUMAN CELL; IN VITRO STUDY; LIGATION MEDIATED POLYMERASE CHAIN REACTION; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN FUNCTION; PROTEIN POLYMERIZATION; PYROCOCCUS FURIOSUS; THERMUS AQUATICUS; CHEMISTRY; CHROMOSOME BREAKAGE; CHROMOSOME MAP; COMPARATIVE STUDY; CONFORMATION; DNA DENATURATION; DRUG EFFECT; ENZYMOLOGY; GC RICH SEQUENCE; GENETICS; GENOME; LYMPHOCYTE; METABOLISM; METHODOLOGY; NUCLEOTIDE SEQUENCE; OSMOLARITY; POLYMERASE CHAIN REACTION; TEMPERATURE; THERMODYNAMICS;

BASE SEQUENCE; CHROMOSOME BREAKAGE; DNA; DNA DAMAGE; DNA LIGASES; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; GC RICH SEQUENCE; GENOME, HUMAN; HUMANS; LYMPHOCYTES; MALE; NUCLEIC ACID CONFORMATION; NUCLEIC ACID DENATURATION; OSMOLAR CONCENTRATION; PHYSICAL CHROMOSOME MAPPING; PIPERIDINES; POLYMERASE CHAIN REACTION; PYROCOCCUS FURIOSUS; SALTS; TAQ POLYMERASE; TEMPERATURE; THERMODYNAMICS;

EID: 0035882940     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/29.16.e83     Document Type: Article

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