Isolation, characterization, and recombinant expression of multiple serpins from the cat flea, Ctenocephalides felis

Archives of Insect Biochemistry and Physiology

Volumn 55, Issue 4, 2004, Pages 200-214

  Brandt, K S ^a   Silver, G M ^a   Becher, A M ^a   Gaines, P J ^a,b   Maddux, J D ^a   Jarvis, E E ^a   Wisnewski, N ^a  

^a HESKA CORPORATION   (United States)

^b NONE   (United States)

Author keywords

Alternate exon splicing; Cat flea; Serpin

Indexed keywords

COMPLEMENTARY DNA; RECOMBINANT PROTEIN; SERINE PROTEINASE INHIBITOR;

ANIMAL; ARTICLE; BIOSYNTHESIS; CAT; CLASSIFICATION; DIGESTIVE SYSTEM; FLEA; GENE; GENE AMPLIFICATION; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; LARVA; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS;

ANIMALS; BASE SEQUENCE; CATS; CLONING, MOLECULAR; DIGESTIVE SYSTEM; DNA, COMPLEMENTARY; FLEAS; GENE AMPLIFICATION; GENE EXPRESSION; GENES, INSECT; LARVA; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SERPINS;

CTENOCEPHALIDES; CTENOCEPHALIDES FELIS; ESCHERICHIA COLI; FELIS; FELIS CATUS; INSECTA; SIPHONAPTERA (FLEAS); UNIDENTIFIED BACULOVIRUS;

EID: 2342631334     PISSN: 07394462     EISSN: None     Source Type: Journal    
DOI: 10.1002/arch.10139     Document Type: Article

References (26)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403-410.
2. Chamankhah M, Braun L, Visal-Shah S, O'Grady M, Baldwin D, Shi X, Hemmingsen SM, Alting-Mees M, Hegedus DD. 2003. Mamestra configurata serpin-1 homologues: cloning, localization and developmental regulation. Insect Biochem Mol Biol 33:355-369
3. Chomczynski P, Sacchi N. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
4. Danielli A, Kafatos FC, Loukeris TG. 2003. Cloning and characterization of four Anopheles gambiae serpin isoforms, differentially induced in the midgut by Plasmodium berghei invasion. J Biol Chem 278:4184-4193
5. Gaines PJ, Sampson, CM, Rushlow, KE, Stiegler, GL. 1999. Cloning of a family of serine protease genes from the cat flea Ctenocephalides felis. Insect Mol Biol 8:11-22.
6. Gan H, Wang Y, Jiang H, Mita K, Kanost MR. 2001. A bacteria-induced, intracellular serpin in granular hemocytes of Manduca sexta. Insect Biochem Mol Biol 31:887-898.
7. Gullan PJ, Cranston PS. 2000. The insects: an outline of entomology, 2nd ed. Oxford, MA: Blackwell Science.
8. Han J-H, Zhang H-Y, Min G-S, Kemler D, Hashimoto C. 2000. A novel Drosophila serpin that inhibits serine proteases. FEBS Let 468:194-198.
9. Jiang H, Kanost MR. 1997. Characterization and functional analysis of 12 naturally occurring reactive site variants of serpin-1 from Manduca sexta. J Biol Chem 272:1082-1087.
10. Jiang H, Wang Y, Kanost MR. 1994. Mutually exclusive exon use and reactive center diversity in insect serpins. J Biol Chem 269:55-58.
11. Jiang H, Mulnix AB, Kanost MR. 1995. Expression and characterization of recombinant Manduca sexta serpin-1B and site-directed mutants that change its inhibitory selectivity. Insect Biochem Mol Biol 25:1093-1100.
12. Jiang H, Wang Y, Huang Y, Mulnix AB, Kadel J, Cole K, Kanost MR. 1996. Organization of serpin gene-1 from Manduca sexta. J Biol Chem 271:28017-28023.
13. Kanost MR. 1990. Serine protease inhibitors from the serpin gene family. In: Hagedorn HH, Hildebrand JG, Kidwell Law JH, editors. Molecular insect science. New York: Plenum Press. p 139-146.
14. Kanost MR. 1999. Serine proteinase inhibitors in arthropod immunity. Dev Comp Immun 23:291-301.
15. Kanost MR, Prasad SV, Wells MA 1989. Primary Structure of a member of the serpin superfamily of proteinase inhibitors from an insect, Manduca sexta. J Biol Chem 264:965-972.
16. Korpula-Mastalerz R, Dubin A. 1996. The intracellular serpin family. Acta Biochim Polon 43:419-430.
17. Kruger O, Ladewig J, Koster K, Ragg H. 2002. Widespread occurrence of serpin genes with multiple reactive centre-containing exon cassettes in insects and namatodes. Gene 293:97-105.
18. Levashina EA, Langley E, Green C, Gubb D, Ashburner M, Hoffman JA, Reichhart J-M. 1999. Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila. Science 285:1917-1919.
19. Miura Y, Kawabata S, Wakamiya Y, Nakamura T, Iwanaga S. 1995. A Limulus intracellular coagulation inhibitor type 2: purification characterization, cDNA cloning and tissue localization. J Biol Chem 270:558-565.
20. Park DS, Shin SW, Hong SD, Park HY. 2000. Immunological detection of serpin in the fall webworm, Hyphantria cunea and its inhibitory activity on the prophenoloxidase system. Mol Cells 10:186-192
21. Polanowski A, Wilusz T, Blum MS, Escoubas PI, Schmidt JO, Travis J. 1992. Serine proteinase inhibitor profiles in the hemolymph of a wide range of insect species. Comp Biochem Physiol B 102:757-760.
22. Potempa J, Korzus E, Travis J. 1994. The serpin superfamily of proteinase inhibitors: structure, function, and regulation. J Biol Chem 269:15957-15960.
23. Sasaki T. 1991. Patchwork-structure serpins from silkworm (Bombyx mori) 1 hemolymph. Eur J Biochem 202:255-261.
24. Thomas RE, Wallenfels L, Popiel I. 1996. On-host viability and fecundity of Ctenocephalides felis (Siphonaptera: Pulididae), using a novel chambered flea technique. J Med Entomol 33:250-256.
25. von Heijne G. 1986. A new method for predicting signal peptide cleavage sites. Nucleic Acids Res 14:4683-4690.
26. Zhou A, Carrell RW, Huntington JA. 2001. The serpin inhibitory mechanism is critically dependent on the length of the reactive center loop. J Biol Chem 276:27541-27547.