Immunological detection of serpin in the fall webworm, Hyphantria cunea and its inhibitory activity on the prophenoloxidase system

Molecules and Cells

Volumn 10, Issue 2, 2000, Pages 186-192

  Park, Doo Sang ^a   Shin, Sang Woon ^a   Hong, Soon Duck ^b   Park, Ho Yong ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Kyungpook National University   (South Korea)

Author keywords

Insect Defense; Phenoloxidase; Serine Protease Inhibitor; Western Blot

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HYPHANTRIA CUNEA; INSECTA; ORYCTOLAGUS CUNICULUS;

CATECHOL OXIDASE; ENZYME INHIBITOR; ENZYME PRECURSOR; HYBRID PROTEIN; INSECT PROTEIN; MONOPHENOL MONOOXYGENASE; PRO PHENOLOXIDASE; PRO-PHENOLOXIDASE; SERINE PROTEINASE INHIBITOR;

ANIMAL; ARTICLE; DRUG ANTAGONISM; ENZYME ACTIVATION; ENZYMOLOGY; GENE; GENETICS; HEMOLYMPH; IMMUNOLOGY; LARVA; LEPIDOPTERA; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT;

ANIMALS; CATECHOL OXIDASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME INHIBITORS; ENZYME PRECURSORS; GENES, INSECT; HEMOLYMPH; INSECT PROTEINS; LARVA; LEPIDOPTERA; MONOPHENOL MONOOXYGENASE; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SERPINS;

EID: 0034732383     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10059-000-0186-2     Document Type: Article

References (41)

1. Agarwala, K. L., Kawabata, S., Miura, Y., Kuroki, Y., and Iwanaga, S. (1996) Limulus intracellular coagulation inhibitor type 3. Purification, characterization, cDNA cloning, and tissue localization. J. Biol. Chem. 271, 23768-23774.
2. Ashida, M. and Brey, P. T. (1997) Recent advances on the research of the insect prophenoloxidase cascade; in Molecular Mechanisms of the Insect Immune Response, Brey, P. T. and Hultmark, D. (eds.), pp. 135-172, Chapman and Hall, London.
3. Ashida, M. and Sasaki, T. (1994) A target protease activity of serpins in insect hemolymph. Insect Biochem. Mol. Biol. 24, 1037-1041.
4. Aspen, A., Huang, T. S., Cernius, L., and Sorderhall, K. (1995) cDNA cloning of prophenoloxidase from the freshwater crayfish Pacifastacus leniusculus and its activation. Proc. Natl. Acad. Sci. USA 92, 936-943.
5. Blanton, R. E., Licate, L. S., and Aman, R. A. (1994) Characterization of a native and recombinant Schistosoma haematobium serine protease inhibitor gene product. Mol. Biochem. Parasitol. 63, 1-11.
6. Boigegrain, R.-A., Mattras, H., Paroutaud, P., and Coletti-Previero, M.-A. (1992) Insect Immunity: Two proteinase inhibitors from hemolymph of Lochsta migratoria. Biochem. Biophys. Res. Commun. 189, 790-793.
7. Brehelin, M., Biogegrain, R. A., Drif., L., and Coletti-Previero, M. A. (1991) Purification of a protease inhibitor which controls prophenoloxidase activation in hemolymph of Locusta migratoria (insecta). Biochem. Biophys. Res. Commun. 179, 841-846.
8. Carrell, R. W. and Boswell, D. R. (1986) Serpins, the superfamily of plasma serine protease inhibitors; in Proteinase Inhibitors, Barrett, A. J. et al. (eds.), pp. 403-420, Elsevier, New York.
9. Chosa, N., Fukumitsu, T., Fujimoto, K., and Ohnish, E. (1997) Activation of prophenoloxidase A1 by an activating enzyme in Drosophila melanogaster. Insect Biochem. Mol. Biol. 27, 61-68.
10. Dohke, K. (1973) Studies on prophenoloxidase-activating enzyme from cuticle of the silkworm, Bombyx mori. II. Purification and characterization of the enzyme. Arch. Biochem. Biophys. 157, 210-221
11. Faye, I. and Hultmark, D. (1993) The insect immune proteins and the regulation of their genes; in Parasites and Pathogens of Insects, Beckage, N. E., Thompson, S. N., and Federici, B. A. (eds.), pp. 25-53, Academic Press Inc, San Diego.
12. Fujimoto, K., Okino, N., Kawabata, S. I., Iwanaga, S., and Ohinish, E. (1995) Nucleotide sequence of the cDNA encoding the proenzyme of phenol oxidase A1 of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 92, 7769-7773.
13. Gettins, P., Patston, P. A., and Schapira, M. (1993) The role of conformation change in serpin structure and function. BioEssays 15, 461-467.
14. Hall, M., Scott, T., Sugumaran, M., Soderhall, K., and Law, J. H. (1995) Proenzyme of Manduca sexta phenol oxidase: Purification, activation, substrate specificity of the active enzyme, and molecular cloning. Proc. Natl. Acad. Sci. USA 92, 7764-7768.
15. Jiang, H. and Kanost, M. R. (1997) Characterization and functional analysis of 12 naturally occurring reactive site variants of serpin-1 from Manduca sexta. J. Biol. Chem. 272, 1082-1087.
16. Jiang, H., Wang, Y., and Kanost, M. R. (1994) Mutually exclusive exon use and reactive center diversity in insect serpin. J. Biol. Chem. 269, 55-58.
17. Jiang, H., Wang, Y., Huang, Y., Mulnix, A. B., Kadel, J., Cole, K., and Kanost, M. R. (1996) Organization of serpin gene-1 from Manduca sexta: evolution of a family of alternate exons encoding the reactive site loop. J. Biol. Chem. 271, 28017-28023.
18. Jiang, H., Wang, Y., Korochkina, S. E., Benes, H., and Kanost, M. R. (1997a) Molecular cloning of cDNAs for two pro-phenol oxidase subunits from the malaria vector, Anopheles gambiae. Insect Biochem. Mol. Biol. 27, 693-699.
19. Jiang, H., Wang, Y., Ma, C., and Kanost, M. R. (1997b) Subunit composition of pro-phenol oxidase from Manduca sexta: molecular cloning of subunit ProPO-P1. Insect Biochem. Mol. Biol. 27, 835-850.
20. Jiang, H., Wang, Y., and Kanost, M. R. (1988) Pro-phenol oxidase activating proteinase from an insect, Manduca sexta: a bacteria-inducible protein similar to Drosophila caster. Proc. Natl. Acad. Sci. USA 95, 12220-12225.
21. Kanost, M. R., Prasad, S. V., and Wells, M. A. (1989) Primary structure of a member of the serpin superfamily of proteinase inhibitors from an insect, Manduca sexta. J. Biol. Chem. 264, 965-972.
22. Kawabata, T., Yasuhara, Y., Ochiai, M., Matsuura, S., and Ashida, M. (1995) Molecular cloning of insect pro-phenol oxidase: containing protein homologous to arthropod hemocyanin. Proc. Natl. Acad. Sci. USA 92, 7774-7778.
23. Kwon, T. H., Lee, S. Y., Lee, J. H., Choi, J. S., Kawabata, S., Iwanaga, S., and Lee, B. L. (1997) Purification and characterization of prophenoloxidase from the hemolymph of coleopteran insect, Holotrichia diomphalia larvae. Mol. Cells 7, 90-97.
24. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
25. Lee, S. Y., Kwon, T. H., Hyun, J. H., Choi, J. S., Kawabata, S.-I., Iwanaga, S., and Lee, B. L. (1998a) In vitro activation of pro-pheriol-oxidase by two kinds of pro-phenol-oxidase-activating factors isolated from hemolymph of coleopteran, Holotrichia diomphalia larvae. Eur. J. Biochem. 254, 50-57.
26. Lee, S. Y., Cho, M. Y., Hyun, J. H., Lee, K. M., Homma, K.-I., Natori, S., Kawabata, S.-I., Iwanaga, S., and Lee, B. L. (1998b) Molecular cloning of cDNA for pro-phenol-oxidase-activating factor I, a serine protease is induced by lipopolysaccharide or 1,3-7beta;-glucan in colopteran insect, Holotrichia diomphalia larvae. Eur. J. Biochem. 257, 615-621.
27. Lee, W.-J., Ahmed, A., Torre A., Kobayashi, A., Ashida, M., and Brey, P. T. (1998) Molecular cloning and chromosomal localization of prophenoloxidase cDNA from the malaria vector, Anopheles gambiae. Insect Mol. Biol. 7, 45-50.
28. Liang, Z. and Soderhall, K. (1995) Isolation of cDNA encoding a novel serpin of crayfish hemocytes. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 112, 385-391.
29. Miura, Y., Kawabata, S., and Iwanaga, S. (1994) A Limulus intracellular coagulation inhibitor with characteristics of the serpin superfamily. Purification, characterization, and cDNA cloning. J. Biol. Chem. 269, 542-547.
30. Miura, Y., Kawabata, S., Wakamiya, Y., Nakamura, T., and Iwanaga, S. (1995) A limulus intracellular coagulation inhibitor type 2. Purification, characterization, cDNA cloning, and tissue localization. J. Biol. Chem. 270, 558-565.
31. Nappi, A. J. and Vass, E. (1993) Melanogenesis and the generation of cytotoxic molecules during insect cellular immune reactions. Pigment Cell Res. 6, 117-126.
32. Narumi, H., Kishida, T., Sasaki, T., Feng, D., and Doolittle, R. F. (1993) Molecular cloning of silkworm (Bombyx mori) antichymotrypshin, a new member of the serpin superfamily of proteins from insects. Eur. J. Biochem. 214, 181-187.
33. Park, D.-S., Shin, S. W., Kim, M. G., Park, S. S., Lee, W. J., Brey, P. T., and Park, H.-Y. (1997) Isolation and characterization of the cDNA encoding the prophenoloxidase of fall webworm, Hyphaniria cunea. Insect Biochem. Mol. Biol. 27, 983-992.
34. Potempa, J., Korzus, E., and Travis, J. (1994) The serpin superfamily of proteinase inhibitors: structure, function, and regulation. J. Biol. Chem. 269, 15957-15960.
35. Sasaki, T. (1991) Patchwork-structure serpins from silkworm (Bombyx mori) larval homolymph. Eur. J. Biochem. 202, 255-261.
36. Sasaki, T. and Kobayashi, K. (1984) Isolation of two novel proteinase inhibitors from hemolymph of silkworm larva. Bombyx mori. Comparision with human serum proteinase inhibitors. J. Biochem. 95, 1009-1017.
37. Satoh, D., Horii, A., Ochiai, M., and Ashida, M. (1999) Prophenoloxidase-activating enzyme of the silkworm, Bombyx mori. Purification, characterization, and cDNA cloning. J. Biol. Chem. 274, 7441-7453.
38. Scheeler, I. and Berger, A. (1967) On the size of the active site in protease. I. Papain. Biochem. Biophys. Res. Commun. 27, 157-162.
39. Shin, S. W., Park, S. S., Park, D.-S., Kim, M. G., Kim, S. C., Brey, P. T., and Park, H.-Y. (1998) Isolation and characterization of immune-related genes from the fall webworm, Hyphantria cunea, using PCR-based differential display and subtractive cloning. Insect Biochem. Mol. Biol. 28, 827-837.
40. Soderhall, K. (1981) Fungal cell wall β-1,3-glucans induce clotting and phenoloxidase attachment to foreign surfaces of crayfish hemocyte lysate. Dev. Comp. Immun. 5 565-573.
41. Sugumaran., M. and Kanost, M. R. (1993) Regulation of insect hemolymph phenoloxidases; in Parasites and Pathogens of Insects, Beckage, N. E., Thompson, S. N., and Federici, B. A. (eds.), pp. 317-342, Academic Press Inc. San Diego.