Computer-Identified Nuclear Localization Signal in Exon 1A of the Transporter DMT1 Is Essentially Ineffective in Nuclear Targeting

Journal of Neuroscience Research

Volumn 76, Issue 4, 2004, Pages 497-511

  Kuo, H C ^a   Smith, J J ^a   Lis, A ^a   Zhao, L ^a   Gonsiorek, E A ^a   Zhou, X ^a   Higgins, D M ^a   Roth, J A ^a   Garrick, Michael D ^a   Garrick, L M ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

DCT1; Ferrous iron; IRE; Metal transport; NLS; Nramp2; SLC11A2

Indexed keywords

NATURAL RESISTANCE ASSOCIATED MACROPHAGE PROTEIN 2;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CELL VACUOLE; CELLULAR DISTRIBUTION; COMPUTER ANALYSIS; EXON; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IRON TRANSPORT; MEMBRANE VESICLE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TARGETING; RAT; SYMPATHETIC NERVE CELL; WESTERN BLOTTING;

ANIMALS; ANIMALS, NEWBORN; BLOTTING, WESTERN; CATION TRANSPORT PROTEINS; CELL MEMBRANE; CELL NUCLEUS; CELLS, CULTURED; CYTOPLASM; EMBRYO; EXONS; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; IRON-BINDING PROTEINS; KIDNEY; LUMINESCENT PROTEINS; NEURONS; NUCLEAR LOCALIZATION SIGNALS; PROTEIN ISOFORMS; PROTEIN TRANSPORT; PROTEINS; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SYMPATHETIC NERVOUS SYSTEM; TRANSFECTION;

EID: 2342574313     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/jnr.20112     Document Type: Article

References (37)

1. Broyles RH, Belegu V, DeWitt CR, Shah SN, Stewart CA, Pye QN, Floyd RA. 2001. Specific repression of β-globin promoter activity by nuclear ferritin. Proc Natl Acad Sci USA 98:9145-9150.
2. Burdo JR, Connor JR. 2003. Brain iron uptake and homeostatic mechanisms: an overview. Biometals 16:63-75.
3. Burdo JR, Martin J, Menzies SL, Dolan KG, Romano MA, Fletcher RJ, Garrick MD, Garrick LM, Connor JR. 1999. Cellular distribution of iron in the brain of the Belgrade rat. Neuroscience 93:1189-1196.
4. Burdo JR, Menzies SL, Simpson IA, Garrick LM, Garrick MD, Dolan KG, Haile DJ, Beard JL, Connor JR. 2001. Distribution of divalent metal transporter 1 and metal transport protein 1 in the normal and Belgrade rat. J Neurosci Res 66:1198-1207.
5. Cai CX, Linsenmayer TF. 2001. Nuclear translocation of ferritin in corneal epithelial cells. J Cell Sci 114:2327-2334.
6. Cai CX, Birk DE, Linsenmayer TF. 1997. Ferritin is a developmentally regulated nuclear protein of avian corneal epithelial cells. J Biol Chem 272:12831-12839.
7. Cai CX, Birk DE, Linsenmayer TF. 1998. Nuclear ferritin protects DNA from UV damage in corneal epithelial cells. Mol Biol Cell 9:1037-1051.
8. Canonne-Hergaux F, Fleming MD, Levy JE, Gauthier S, Ralph T, Picard V, Andrews NC, Gros P. 2000. The Nramp2/DMT1 iron transporter is induced in the duodenum of microcytic anemia mk mice but is not properly targeted to the intestinal brush border. Blood 96:3964-3970.
9. Dingwall C, Laskey RA. 1991. Nuclear targeting sequences-a consensus. TIBS 16:478-481.
10. Ferguson CJ, Wareing M, Ward DT, Green R, Smith CP, Riccardi D. 2001. Cellular localization of divalent metal transporter DMT-1 in rat kidney. Am J Physiol Renal Physiol 280:F803-F814.
11. Fleming MD, Trenor CI, Su MA, Foernzler D, Beier DR, Dietrich WF, Andrews NC. 1997. Microcytic anaemia mice have a mutation in Nramp2, a candidate iron transporter gene. Nat Genet 16:383-386.
12. Fleming MD, Romano MA, Su MA, Garrick LM, Garrick MD, Andrews NC. 1998. Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport. Proc Nad Acad Sci USA 95:1148-1153.
13. Garrick MD, Garrick LM. 2004. Divalent metal transporter DMT1 (SLC11A2). In: Broer S, Wagner C, editors. Membrane transporter diseases. Dordrecht: Kluwer. p 147-154.
14. Garrick MD, Dolan KG, Ghio A, Horbinski C, Higgins D, Porubcin M, Moore EG, Hainsworth LN, Umbreit JN, Conrad ME, Feng L, Lis A, Roth JE, Singleton S, Garrick LM. 2003. DMT1 (divalent metal transporter 1): a mammalian transporter for multiple metals. Biometals 16:41-54.
15. Geuens E, Brouns I, Flamez D, Dewilde S, Timmermans JP, Moens L. 2003. A globin in the nucleus! J Biol Chem 278:30417-30420.
16. Gruenheid S, Canonne-Hergaux F, Gauthier S, Hackam DJ, Grinstein S, Gros P. 1999. The iron transport protein NRAMP2 is an integral membrane glycoprotein that colocalizes with transferrin in recycling endosomes. J Exp Med 189:831-841.
17. Gunshin H, Mackenzie B, Berger UV, Gunshin Y, Romero MF, Boron WF, Nussberger S, Gollan JL, Hediger MA. 1997. Cloning and characterization of a mammalian proton-coupled metal-ion transporter. Nature 388:482-488.
18. Hanada K, Yewdell JW, Yang JC. 2004. Immune recognition of a human renal cancer antigen through post-translational splicing. Nature 427:252-256.
19. Higgins D, Lein PJ, Osterhout DJ, Johnson MI. 1991. In: Banker G, Goslin K, editors. Culturing nerve cells. Cambridge, MA: MIT Press. p 177-206.
20. Horbinski C, Kim W-Y, Sigurdson W, Higgins D. 2004. Proteasome inhibitors suppress formation of polyglutamine-induced nuclear inclusions in cultured postmitotic neurons. Submitted for publication.
21. Hubert N, Hentze MW. 2002. Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: implications for regulation and cellular function. Proc Natl Acad Sci USA 99:12345-12350.
22. Jans DA, Xiao C-Y, Lam MH. 2000. Nuclear targeting signal recognition: A key control point in nuclear transport? Bioessays 22:532-544.
23. Keresztes M, Boonstra J. 1999. Import(ance) of growth factors in(to) the nucleus. J Cell Biol 145:421-424.
24. Komeili A, O'Shea EK. 2001. New perspectives on nuclear transport. Annu Rev Genet 35:341-364.
25. Millholland JM, Fitch JM, Cai CX, Gibney EP, Linsenmayer TF. 2003. Ferritoid: a tissue-specific nuclear transport protein for ferritin in corneal epithelial cells. J Biol Chem 278:23963-23970.
26. Nakielny S, Dreyfuss G. 1999. Transport of proteins and RNAs in and out of the nucleus. Cell 99:677-690.
27. Pang H, Bartlam M, Zeng Q, Miyatake H, Hisano T, Miki K, Wong L, Gao GF, Rao Z. 2004. Crystal structure of human pirin. An iron-binding nuclear protein and transcription cofactor. J Biol Chem 279:1491-1498.
28. Pigeon C, Ilyin G, Courselaud B, Leroyer P, Turlin B, Brissot P, Loreal O. 2001. A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J Biol Chem 276:7811-7819.
29. Pountney D, Trugnan G, Bourgeois M, Beaumont C. 1999. The identification of ferritin in the nucleus of K562 cells, and investigation of a possible role in the transcriptional regulation of adult beta-globin gene expression. J Cell Sci 112:825-831.
30. Roth JA, Horbinski C, Feng L, Dolan KG, Higgins D, Garrick MD. 2000. Differential localization of divalent metal transporter 1 with and without iron response element in rat PC12 and sympathetic neuronal cells. J Neurosci 20:7595-7601.
31. Suttner DM, Sridhar K, Lee CS, Tomura T, Hansen TN, Dennery PA. 1999. Protective effects of transient HO-1 overexpression on susceptibility to oxygen toxicity in lung cells. Am J Physiol Lung Cell Mol Physiol 276:L443-L451.
32. Tabuchi M, Tanaka N, Nishida-Kitayama JO, Kishi F. 2002. Alternative splicing regulates the subcellular localization of divalent metal transporter 1 isoforms. Mol Biol Cell 13:4371-4387.
33. Talcott B, Moore MS. 1999. Getting across the nuclear pore complex. Trends Cell Biol 9:312-318.
34. Teasdale RD, Jackson MR. 1996. Signal mediated sorting of membrane proteins between the endoplasmic reticulum and the Golgi apparatus. Annu Rev Cell Dev Biol 12:27-54.
35. Thompson KJ, Fried MG, Ye Z, Boyer P, Connor JR. 2002. Regulation, mechanisms and proposed function of ferritin translocation to cell nuclei. J Cell Sci 115:2165-2177.
36. White KN, Graham DC, Bomford A. 2001. Nuclear localisation of iron regulatory proteins. Biochem Soc Trans 30:A38.
37. Yeh KY, Yeh M, Watkins JA, Rodriguez PJ, Glass J. 2000. Dietary iron induces rapid changes in rat intestinal divalent metal transporter expression. Am J Physiol Gastrointest Liver Physiol 279:G1070-G1079.