Ssu72 is an RNA polymerase II CTD phosphatase

Molecular Cell

Volumn 14, Issue 3, 2004, Pages 387-394

  Krishnamurthy, Shankarling ^a   He, Xiaoyuan ^b   Reyes Reyes, Mariela ^a   Moore, Claire ^b   Hampsey, Michael ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHATASE; POLYADENYLATED RNA; PROTEIN S; PROTEIN S 5; RECOMBINANT ENZYME; RNA POLYMERASE II; SMALL SUBUNIT RIBOSOMAL RNA; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; DEPHOSPHORYLATION; ENZYME SPECIFICITY; HUMAN; POLYADENYLATION; PROTEIN DEPLETION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN POLYMERIZATION; PROTEIN PROCESSING; RNA CLEAVAGE; TRANSCRIPTION REGULATION; TRANSCRIPTION TERMINATION; YEAST;

CARRIER PROTEINS; GENES, REGULATOR; MRNA CLEAVAGE AND POLYADENYLATION FACTORS; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN STRUCTURE, TERTIARY; RNA POLYMERASE II; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE; SUBSTRATE SPECIFICITY; TRANSCRIPTION, GENETIC;

EID: 2342533807     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(04)00235-7     Document Type: Article

References (44)

1. Ahn S.H., Kim M., Buratowski S. Phosphorylation of serine 2 within the RNA polymerase II C-terminal domain couples transcription and 3′ end processing. Mol. Cell. 13:2004;67-76
2. Archambault J., Chambers R.S., Kobor M.S., Ho Y., Cartier M., Bolotin D., Andrews B., Kane C.M., Greenblatt J. An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 94:1997;14300-14305
3. Calvo O., Manley J.L. Strange bedfellows. polyadenylation factors at the promoter Genes Dev. 17:2003;1321-1327
4. Chen B.-S., Hampsey M. Functional interaction between TFIIB and the Rpb2 subunit of RNA polymerase II. implications for the mechanism of transcription initiation Mol. Cell. Biol. 24:2004;3983-3991
5. Chen J., Moore C. Separation of factors required for cleavage and polyadenylation of yeast pre-mRNA. Mol. Cell. Biol. 12:1992;3470-3481
6. Cho E.J., Kobor M.S., Kim M., Greenblatt J., Buratowski S. Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. Genes Dev. 15:2001;3319-3329. a
7. Cho H., Wang W., Kim R., Yokota H., Damo S., Kim S.H., Wemmer D., Kustu S., Yan D. BeF(3)(-) acts as a phosphate analog in proteins phosphorylated on aspartate. structure of a BeF(3)(-) complex with phosphoserine phosphatase Proc. Natl. Acad. Sci. USA. 98:2001;8525-8530. b
8. Corden J.L. Tails of RNA polymerase II. Trends Biochem. Sci. 15:1990;383-387
9. Dahmus M.E. Reversible phosphorylation of the C-terminal domain of RNA polymerase II. J. Biol. Chem. 271:1996;19009-19012
10. Dantonel J.C., Murthy K.G., Manley J.L., Tora L. Transcription factor TFIID recruits factor CPSF for formation of 3′ end of mRNA. Nature. 389:1997;399-402
11. Dichtl B., Blank D., Ohnacker M., Friedlein A., Roeder D., Langen H., Keller W. A role for SSU72 in balancing RNA polymerase II transcription elongation and termination. Mol. Cell. 10:2002;1139-1150
12. Dohmen R.J., Wu P., Varshavsky A. Heat-inducible degron. a method for constructing temperature-sensitive mutants Science. 263:1994;1273-1276
13. Flint A.J., Tiganis T., Barford D., Tonks N.K. Development of "substrate-trapping" mutants to identify physiological substrates of protein tyrosine phosphatases. Proc. Natl. Acad. Sci. USA. 94:1997;1680-1685
14. Ganem C., Devaux F., Torchet C., Jacq C., Quevillon-Cheruel S., Labesse G., Facca C., Faye G. Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast. EMBO J. 22:2003;1588-1598
15. Gavin A.C., Bosche M., Krause R., Grandi P., Marzioch M., Bauer A., Schultz J., Rick J.M., Michon A.M., Cruciat C.M., et al. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature. 415:2002;141-147
16. Hampsey M., Reinberg D. Tails of intrigue. phosphorylation of RNA polymerase II mediates histone methylation Cell. 113:2003;429-432
17. Hausmann S., Shuman S. Characterization of the CTD phosphatase Fcp1 from fission yeast. Preferential dephosphorylation of serine 2 versus serine 5. J. Biol. Chem. 277:2002;21213-21220
18. He X., Khan A.U., Cheng H., Pappas D.L. Jr., Hampsey M., Moore C.L. Functional interactions between the transcription and mRNA 3′ end processing machineries mediated by Ssu72 and Sub1. Genes Dev. 17:2003;1030-1042
19. Kobor M.S., Archambault J., Lester W., Holstege F.C., Gileadi O., Jansma D.B., Jennings E.G., Kouyoumdjian F., Davidson A.R., Young R.A., et al. An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae. Mol. Cell. 4:1999;55-62
20. Kobor M.S., Simon L.D., Omichinski J., Zhong G., Archambault J., Greenblatt J. A motif shared by TFIIF and TFIIB mediates their interaction with the RNA polymerase II carboxy-terminal domain phosphatase Fcp1p in Saccharomyces cerevisiae. Mol. Cell. Biol. 20:2000;7438-7449
21. Komarnitsky P., Cho E.J., Buratowski S. Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription. Genes Dev. 14:2000;2452-2460
22. Lee J.M., Greenleaf A.L. Modulation of RNA polymerase II elongation efficiency by C-terminal heptapeptide repeat domain kinase I. J. Biol. Chem. 272:1997;10990-10993
23. Lin P.S., Dubois M.F., Dahmus M.E. TFIIF-associating carboxyl-terminal domain phosphatase dephosphorylates phosphoserines 2 and 5 of RNA polymerase II. J. Biol. Chem. 277:2002;45949-45956
24. Maldonado E., Drapkin R., Reinberg D. Purification of human RNA polymerase II and general transcription factors. Methods Enzymol. 274:1996;72-100
25. Meinhart A., Silberzahn T., Cramer P. The mRNA transcription/processing factor ssu72 is a potential tyrosine phosphatase. J. Biol. Chem. 278:2003;15917-15921
26. Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J. Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3′-ends. J. Biol. Chem. 278:2003;33000-33010
27. Orphanides G., Reinberg D. A unified theory of gene expression. Cell. 108:2002;439-451
28. Pappas D.L. Jr., Hampsey M. Functional interaction between Ssu72 and the Rpb2 subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol. Cell. Biol. 20:2000;8343-8351
29. Patturajan M., Schulte R.J., Sefton B.M., Berezney R., Vincent M., Bensaude O., Warren S.L., Corden J.L. Growth-related changes in phosphorylation of yeast RNA polymerase II. J. Biol. Chem. 273:1998;4689-4694
30. Prelich G. RNA polymerase II carboxy-terminal domain kinases. emerging clues to their function Eukaryot. Cell. 1:2002;153-162
31. Rodriguez C.R., Cho E.J., Keogh M.C., Moore C.L., Greenleaf A.L., Buratowski S. Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II. Mol. Cell. Biol. 20:2000;104-112
32. Sanders S.L., Jennings J., Canutescu A., Link A.J., Weil P.A. Proteomics of the eukaryotic transcription machinery. identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry Mol. Cell. Biol. 22:2002;4723-4738
33. Schroeder S.C., Schwer B., Shuman S., Bentley D. Dynamic association of capping enzymes with transcribing RNA polymerase II. Genes Dev. 14:2000;2435-2440
34. Sharma S.K., Carew T.J. Inclusion of phosphatase inhibitors during Western blotting enhances signal detection with phospho-specific antibodies. Anal. Biochem. 307:2002;187-189
35. Shatkin A.J., Manley J.L. The ends of the affair. capping and polyadenylation Nat. Struct. Biol. 7:2000;838-842
36. Siniossoglou S., Hurt E.C., Pelham H.R. Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast. J. Biol. Chem. 275:2000;19352-19360
37. Steinmetz E.J., Brow D.A. Ssu72 protein mediates both poly(A)-coupled and poly(A)-independent termination of RNA polymerase II transcription. Mol. Cell. Biol. 23:2003;6339-6349
38. Sun Z.-W., Hampsey M. Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential gene encoding a novel protein that affects transcription start site selection in vivo. Mol. Cell. Biol. 16:1996;1557-1566
39. Woontner M., Jaehning J.A. Accurate initiation by RNA polymerase II in a whole cell extract from Saccharomyces cerevisiae. J. Biol. Chem. 265:1990;8979-8982
40. Wu W.-H., Pinto I., Chen B.-S., Hampsey M. Mutational analysis of yeast TFIIB. a functional relationship between Ssu72 and Sub1/Tsp1 defined by allele-specific interactions with TFIIB Genetics. 153:1999;643-652
41. Yeo M., Lin P.S., Dahmus M.E., Gill G.N. A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J. Biol. Chem. 278:2003;26078-26085
42. Young R.A. RNA polymerase II. Annu. Rev. Biochem. 60:1991;689-715
43. Zhang J., Corden J.L. Identification of phosphorylation sites in the repetitive carboxyl-terminal domain of the mouse RNA polymerase II largest subunit. J. Biol. Chem. 266:1991;2290-2296
44. Zhao J., Kessler M., Helmling S., O'Connor J.P., Moore C. Pta1, a component of yeast CF II, is required for both cleavage and poly(A) addition of mRNA precursor. Mol. Cell. Biol. 19:1999;7733-7740