Inclusion of phosphatase inhibitors during Western blotting enhances signal detection with phospho-specific antibodies

Analytical Biochemistry

Volumn 307, Issue 1, 2002, Pages 187-189

  Sharma, Shiv K ^a   Carew, Thomas J ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SIGNAL DETECTION;

PHOSPHATASE INHIBITORS; WESTERN BLOTTING;

CHEMICAL DETECTION;

BOVINE SERUM ALBUMIN; ENZYME INHIBITOR; FLUORIDE SODIUM; PHOSPHATASE; VANADATE SODIUM; IMMUNOGLOBULIN G; PHOSPHOPROTEIN PHOSPHATASE; VANADIC ACID;

ANTIBODY DETECTION; ARTICLE; CONCENTRATION RESPONSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; SIGNAL DETECTION; WESTERN BLOTTING; ANIMAL; APLYSIA; DRUG ANTAGONISM; DRUG EFFECT; IMMUNOLOGY; METABOLISM; METHODOLOGY; PHOSPHORYLATION; PLEURA CAVITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SIGNAL TRANSDUCTION;

BOVINAE;

ANIMALS; APLYSIA; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; IMMUNOGLOBULIN G; MAP KINASE SIGNALING SYSTEM; NEURONS; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PLEURAL CAVITY; SODIUM FLUORIDE; VANADATES;

EID: 0036667931     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00008-8     Document Type: Article

References (5)

1. Protein kinases and phosphatases: The yin and yang of protein phosphorylation and signaling
2. Increased histone acetyltransferase and lysine acetyltransferase activity and biphasic activation of the ERK/RSK cascade in insular cortex during novel taste learning
3. Inhibition of mitogen-activated protein kinase activity and proliferation of an early osteoblast cell line (MBA 15.4) by dexamethasone: Role of protein phosphatases
4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
5. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications