Expression and purification of polyhistidine-tagged firefly luciferase in insect cells - A potential alternative for process scale-up

Journal of Biotechnology

Volumn 85, Issue 1, 2001, Pages 49-56

  Michel, Patrik ^a,c   Torkkeli, Tuula ^a   Karp, Matti ^b   Oker Blom, Christian ^c  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b UNIVERSITY OF TURKU   (Finland)

^c UNIVERSITY OF JYVÄSKYLÄ   (Finland)

Author keywords

Baculovirus; High Five ; IMAC; Photinus pyralis; Polyhistidine tag; Streamline

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; ENZYME IMMOBILIZATION; ENZYME KINETICS; PURIFICATION; VIRUSES;

BACULOVIRUS;

BIOTECHNOLOGY;

ADENOSINE TRIPHOSPHATE; ADENYLATE KINASE; CREATINE KINASE; LUCIFERASE; METAL ION; NUCLEOSIDE DIPHOSPHATE KINASE; POLYHISTIDINE; VIRUS VECTOR;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ARTICLE; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; CELL CULTURE; CONTROLLED STUDY; DNA MODIFICATION; ENZYME PURIFICATION; INSECT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION;

ANIMALS; BEETLES; HISTIDINE; LUCIFERASES; MOTHS; PEPTIDES; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

EID: 0035936628     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00377-1     Document Type: Article

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