Application of Physical Force Is Essential to Enrich for Epidermal Stem Cells in Primary Human Keratinocyte Isolation

Tissue Engineering

Volumn 10, Issue 3-4, 2004, Pages 343-351

  Park, Hyun Sook ^a   Kang, Hyun Joo ^b   Kim, Chang Hwan ^a   Kim, Chun Ho ^a   Han, Eun Sook ^b   Han, Kyubo ^b   Kim, Tae Hwan ^a   Gin, Yong Jae ^a   Son, Young Sook ^a,c  

^a Korea Atomic Energy Research Institute   (South Korea)

^b RandD Institute   (South Korea)

^c Korea Cancer Center Hospital   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BETA1 INTEGRIN; DISPASE; EDETIC ACID; INVOLUCRIN; KALININ; THERMOLYSIN; TRYPSIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; BASAL CELL; CELL ISOLATION; CELL SELECTION; COLONY FORMATION; CONFERENCE PAPER; CONTROLLED STUDY; EPIDERMIS CELL; FIBROBLAST; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMPLANTATION; KERATINOCYTE; MAGNETIC SEPARATION; MALE; MOUSE; NONHUMAN; NUDE MOUSE; PRIORITY JOURNAL; STEM CELL;

ANIMALS; CELL SEPARATION; COLONY-FORMING UNITS ASSAY; EPIDERMIS; HUMANS; IMMUNOHISTOCHEMISTRY; INTEGRINS; KERATINOCYTES; MAGNETICS; MICE; STEM CELLS; TISSUE ENGINEERING;

EID: 2342466079     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/107632704323061708     Document Type: Conference Paper

References (14)

1. Rheinwald, J.G., and Green, H. Trypsin/EDTA, H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 6, 331, 1975.
2. Germain, L., Rouabhia, M., Guignard, R., Carrier, L., Bouvard, V., and Auger, F.A. Improvement of human keratinocyte isolation and culture using thermolysin. Bums 19, 99, 1993.
3. Walzer, C., Benathan, M., and Frenk, E. Thermolysin treatment: A new method for dermo-epidermal separation. J. Invest. Dermatol. 92, 78, 1989.
4. Rheinwald, J.G., and Green, H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 265, 421, 1977.
5. Burke, J.F., Yannas, I.V., Quinby, W.C., Bondoc, C.C., and Jung, W.K. Successful use of a physiologically acceptable artificial skin in the treatment of extensive bum injury. Ann. Surg. 194, 413, 1981.
6. Heenen, M., and Galand, P. The growth fraction of normal human epidermis. Dermatology 194, 313, 1997.
7. Labker, R.M., and Sun, T. Epidermal stem cells. Proc. Natl. Acad. Sci. U.S.A. 97, 13474, 2000.
8. Bickenbach, J.R., and Chism, E. Selection and extended growth of murine epidermal stem cells in culture. Exp. Cell Res. 244, 184, 1998.
9. Watt, F.M. Epidermal stem cells: Markers, patterning and the control of stem cell fate. Philos. Trans. R. Soc. Lond. B 353, 831, 1998.
10. Wang C.K., Nelson C.F., Brinkman A.M., Miller A.C., and Hoeffler W.K. Spontaneous cell sorting of fibroblasts and keratinocytes creates an organotypic human skin equivalent. J. Invest. Dermatol. 114, 674, 2000.
11. Barrandon, Y., and Green, H. Three clonal types of keratinocyte with different capacities for multiplication. Proc. Natl. Acad. Sci. U.S.A. 84, 2302, 1987.
12. Jones, P., and Watt, F. Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 73, 713, 1993.
13. 1 Integrins regulate keratinocyte adhesion and differentiation by distinct mechanisms. Mol. Biol. Cell 11, 453, 2000.
14. 1integrin in skin: Severe defects in epidermal proliferation, basement membrane formation, and hair follicle invagination. J. Cell Biol. 150, 1149, 2000.