Spontaneous cell sorting of fibroblasts and keratinocytes creates an organotypic human skin equivalent

Journal of Investigative Dermatology

Volumn 114, Issue 4, 2000, Pages 674-680

  Wang, C Kathy ^a   Nelson, Charlotte F ^a   Brinkman, Alice M ^a   Miller, Anne C ^a   Hoeffler, Warren K ^a,b  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Xgene Corporation   (United States)

Author keywords

Artificial skin; Cell adhesion; Skin grafting; Wound healing

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; ARTIFICIAL SKIN; CELL ADHESION; CELL SELECTION; FIBROBLAST; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; MODEL; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; SCID MOUSE; SKIN GRAFT; SKIN TRANSPLANTATION; WOUND HEALING;

EID: 0034002484     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1523-1747.2000.00938.x     Document Type: Article

References (42)

1. Archer SB, Henke A, Greenhalgh DG, Warden: GD: The use of sheet autografts to cover extensive burns in patients. J Burn Care Rehab 19:33-38, 1998
2. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji TA: Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 211:1042-1054, 1981
3. Bell E, Sher S, Hull B, et al: The reconstitution of living skin. J Invest Derm 81:2S-10S, 1983
4. Ben-Bassat H, Eldad A, Chaouat M, Livoff A, Ron N, Ne'eman Z, Wexler MR: Structural and functional evaluation of modifications in the composite skin graft: cryopreserved dermis and cultured keratinocytes. Plast Reconstr Surg 89:510-520, 1990
5. Boukamp P, Rupniak HT, Fusenig NE: Environmental modulation of the expression of differentiation and malignancy in six human squamous cell carcinoma cell lines. Cancer Res 45:5582-5592, 1985
6. Boyce ST, Greenhalgh DG, Kagan RJ, et al: Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers. Plast Reconstr Surg 9:632-641, 1993
7. Burke JF, Yannas IV, Quinby WC, Bondoc CC Jr, Jung WK: Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg 194:413-420, 1981
8. Cooper M, Hansbrough J: Use of a composite graft composed of cultured human keratinocytes and fibroblasts on a collagen-GAG skin matrix to cover full-thickness wounds on athymic mice. Surgery 109:198-207, 1991
9. Cuono C, Langdon R, McGuire J: Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. Lancet 1:1123-1124, 1986
10. Fine JD, Bauer EA, Briggaman RA, Carter DM, Eady RAJ, Esterly NB: Revised clinical and laboratory criteria for subtypes of inherited epidermolysis bullosa. J Am Acad Dermatol 24: 119-135, 1991
11. Fusenig NE, Amer SM, Boukamp P, Worst PK: Characteristics of chemically transformed mouse epidermal cells in vitro and in vivo. Bull Cancer 65:271-280, 1978
12. Fusenig NE, Breitkreutz D, Dzarileva RT, Boukamp P, Bohnert A, Tilgen W: Growth and differentiation characteristics of transformed keratinocytes from mouse and human skin in vitro and in vivo. J Invest Dermatol 81:168s-175s, 1983
13. Green H, Kehinde O, Thomas J: Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 76:5665-5668, 1979
14. Hansbrough JF, Boyce ST, Cooper ML, Foreman TL: Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate. JAMA 262:2125-2130, 1989
15. Hyatt MA: Basic Techniques for Transmission Electron Microscopy. Orlando, FL: Academic Press, 1986, pp. 56-68
16. Inoue M, Kratz G, Haegerstrand A, Stahle-Backdahl M: Collagenase expression is rapidly induced in wound edge keratinocytes after acute injury in human skin; persists during healing and stops at re-epithelialization. J Invest Dermatol 104:479-483, 1995
17. Kinsella TM, Nolan GP: Episomal vectors rapidly and stably produce high-titer recombinant retrovirus. Hum Gene Ther 7:1405-1413, 1996
18. Krejci NC, Cuono CB, Langdon RC, McGuire J: In vitro reconstitution of skin: fibroblasts facilitate keratinocyte growth and differentiation on acellular reticular dermis. J Invest Dermatol 91:478-485, 1991
19. Langdon RC, Cuono CB, Birchall N, Madri JA, Kukinska E, Mcguire J, Moelmann: G: Reconstitution of structure and cell function in human skin grafts derived from cryopreserved allogenic dermis and autologous cultured keratinocytes. J Invest Dermatol 91:478-485, 1988
20. Leigh IM, Purkis PE: LH7:2 a new monoclonal antibody to a lamina densa protein. J Invest Dermatol 84:448-449, 1985
21. Mackenzie IC, Fusenig NE: Regeneration of organized epithelial structure. J Invest Dermatol 81:189s-194s, 1983
22. Matouskova E, Vogtova D, Konigova R: A recombined skin composed of human keratinocytes cultured on cell-free pig dermis. Burn 19:118-123, 1993
23. Matsui C, Wang CK, Nelson CF, Bauer EA, Hoeffler WK: The assembly of laminin-5 subunits. J Biol Chem 270:23496-23503, 1995
24. Matsui C, Pereira P, Wang CK, et al: Extent of laminin-5 assembly and secretion affect junctional epidermolysis bullosa phenotype. J Exp Med 187:1273-1283, 1998
25. Medalie DA, Eming SA, Tompkins RG, Yarmush ML, Krueger GG, Morgan JR: Evaluation of human skin reconstituted from composite grafts of cultured keratinocytes and human acellular dermis transplanted to athymic mice. J Invest Dermatol 107:121-127, 1996
26. Moscona A, Moscona H: Dissociation and aggregation of cells from organ rudiments of the early chick embryos. J Anat 86:287-301, 1952
27. Normand J, Karasek MA: A method for the isolation and serial propagation of keratinocytes, endothelial cells, and fibroblasts from a single punch biopsy of human skin. In Vitro Cellular Dev Biology 31:447-455, 1992
28. Odyssey R: Multicenter experience with cultured epidermal autographs for treatment of burns. J Burn Care Rehabil 13:174-180, 1992
29. Peterson MJ, Woodley DT, Stricklin GP, O'Keefe EJ: Enhanced synthesis of collagenase by human keratinocytes cultured on type I or type IV collagen. J Invest Dermatol 94:341-346, 1990
30. Peterson MJ, Woodley DT, Stricklin GP, O'Keefe EJ: Synthesis and regulation of keratinocyte collagenase. Matrix 1:192-197, 1992
31. Phillips TJ: New skin for old. Arch Dermatol 134:344-349, 1998
32. Philips TJ, Gilchrest BA: Clinical applications of cultured epithelium. Epith Cell Biol 1:39-46, 1992
33. Phillips TJ, Bhawan TJ, Leigh IM, Baum HJ, Gilchrest BA: Cultured epidermal allografts: a study of differentiation and allograft survival. J Am Acad Dermatol 23:189-198, 1990
34. Saarialho-Kere UK, Kovacs SO, Pentland AP, Olerud JE, Welgus HG, Parks WC: Cell-matrix interactions modulate interstitial collagenase expression by human keratinocytes actively involved in wound healing. J Clin Invest 92:2858-2866, 1993
35. Shimoyama Y, Hirohashi S, Hirano S, Noguchi M, Shimosato Y, Takeichi MM, Abe O: Cadherin cell-adhesion molecules in human epithelial tissues and carcinomas. Cancer Res 49:2128-2133, 1989
36. Sudbeck BD, Parks WC, Welgus HG, Pentland AP: Collagen-stimulated induction of keratinocyte collagenase is mediated via tyrosine kinase and protein kinase C activities. J Biol Chem 269:30022-30029, 1994
37. Townes PL, Holtfreter J: Directed movements and selective adhesion of embryonic amphibian cells. J Exp Zool 128:53-120, 1955
38. Weiss P, Taylor AC: Reconstitution of complete organs from single-cell suspensions of chick embryos in the advanced stages of differentiation. Proc Natl Acad Sci USA 46:1177-1185, 1960
39. Woodley DT, Kalebec T, Banes AJ, Link W, Prunieras M, Liotta L: Adult human keratinocytes migrating over nonviable dermal collagen produce collagenolytic enzymes that degrade type I and type IV collagen. J Invest Dermatol 86:418-423, 1986
40. Woodley DT, Peterson HD, Herzog SR et al: Burn wounds resurfaced by cultured epidermal autografts show abnormal reconstitution of anchoring fibrils. JAMA 259:2566-2571, 1988
41. Woodley DT, Briggaman RA, Herzog SR, Meyers AA, Peterson HD, O'Keefe EJ: Characterization of "neo-dermis" formation beneath cultured human epidermal autografts transplanted on muscle fascia. J Invest Dermatol 95:20-26, 1990
42. Worst PKM, Mackenzie IC, Fusenig NE: Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells. Cell Tissue Res 225:65-77, 1982