Functional mapping of charged residues of the 82-116 sequence in factor Xa: Evidence that lysine 96 is a factor Va independent recognition site for prothrombin in the prothrombinase complex

Biochemistry

Volumn 44, Issue 30, 2005, Pages 10063-10070

  Manithody, Chandrashekhara ^a   Rezaie, Alireza R ^a  

^a SAINT LOUIS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; PROTEINS; STICTION; X RAY CRYSTALLOGRAPHY;

CHARGED RESIDUES; FUNCTIONAL MAPPING; MUTATION; PROTHROMBIN;

MAPPING;

ARGININE; ASPARTIC ACID; BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 5A; GLUTAMIC ACID; LYSINE; PROTEINASE; PROTHROMBIN; SODIUM ION;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; CRYSTAL STRUCTURE; ENERGY TRANSFER; HUMAN; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; MUTATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; X RAY ANALYSIS;

ALANINE; AMIDES; ANTITHROMBINS; CATALYSIS; CELL LINE; FACTOR VA; FACTOR XA; HUMANS; HYDROLYSIS; LYSINE; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PROTEIN STRUCTURE, TERTIARY; PROTHROMBIN; SUBSTRATE SPECIFICITY; THROMBOPLASTIN;

EID: 23044453168     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0508791     Document Type: Article

References (30)

1. Nesheim, M. E., Kettner, C., Shaw, E., and Mann, K. G. (1981) Cofactor dependence of factor Xa incorporation into the prothrombinase complex, J. Biol. Chem. 256, 6537-6540.
2. Rosing, J., Tans, G., Govers-Riemslag, J. W. P., Zwaal, R. F. A., and Hemker, H. C. (1930) The role of phospholipids and factor Va in the prothrombinase complex, J. Biol. Chem. 255, 274-283.
3. Furie, B., and Furie, B. C. (1988) The molecular basis of blood coagulation, Cell 53, 505-518.
4. Davie, E. W., Fujikawa, K., and Kisiel, W. (1991) The coagulation cascade: Initiation, maintenance and regulation, Biochemistry 30, 10363-10370.
5. Jackson, C. M., and Nemerson, Y. (1980) Blood coagulation, Annu. Rev. Biochem. 49, 765-811.
6. Mann, K. G., Nesheim, M. E., Church, W. R., Haley, P., and Krishnaswamy, S. (1990) Surface-dependent reactions of the vitamin K-dependent enzyme complexes, Blood 76, 1-16.
7. Dharmawardana, K. R., Olson, S. T., and Bock, P. E. (1999) Role of regulatory exosite I in binding of thrombin to human factor V, factor Va, factor Va subunits, and activation fragments, J. Biol. Chem. 274, 18635-18643.
8. Anderson, P. J., Nesset, A., Dharmawardana, K. R., and Bock, P. E. (2000) Role of proexosite I in factor Va-dependent substrate interactions of prothrombin activation, J. Biol. Chem. 275, 16435-16442.
9. Chen, L., Yang, L., and Rezaie, A. R. (2003) Proexosite on prothrombin is a factor Va-dependent recognition site for the prothrombinase complex, J. Biol. Chem. 278, 27564-27569.
10. Beck, D. O., Bukys, M. A., Singh, L. S., Szabo, K. A., and Kalafatis, M. (2004) The contribution of amino acid region Asp695-Tyr698 of factor V to procofactor activation and factor Va function, J. Biol. Chem. 279, 3084-3095.
11. Yegneswaran, S., Mesters, R. M., and Griffin, J. H. (2003) Identification of distinct sequences in human blood coagulation factor Xa and prothrombin essential for substrate and cofactor recognition in the prothrombinase complex, J. Biol. Chem. 278, 33312-33318.
12. Yegneswaran, S., Mesters, R. M., Fernandez, J. A., and Griffin, J. H. (2004) Prothrombin residues 473-487 contribute to factor Va binding in the prothrombinase complex, J. Biol. Chem. 279, 49019-49025.
13. Betz, A., and Krishnaswamy, S. (1998) Regions remote from the site of cleavage determine macromolecular substrate recognition by the prothrombinase complex, J. Biol. Chem. 273, 10709-10718.
14. Wilkens, M., and Krishnaswamy, S. (2002) The contribution of factor Xa to exosite-dependent substrate recognition by prothrombinase, J. Biol. Chem. 277, 9366-9374.
15. Krishnaswamy, S. (2004) Exosite-driven substrate specificity and function in coagulation, J. Thromb. Haemostasis 3, 54-67.
16. Padmanabhan, K., Padmanabhan, K. P., Tulinsky, A., Park, C. H., Bode, W., Huber, R., Blankenship, D. T., Cardin, A. D., and Kisiel, W. (1993) Structure of human des (1-45) factor Xa at 2.2 Å resolution, J. Mol. Biol. 232, 947-966.
17. Manithody, C., Yang, L., and Rezaie, A. R. (2002) Role of basic residues of the autolysis loop in the catalytic function of factor Xa, Biochemistry 41, 6780-6788.
18. Bode, W., Mayr, I., Baumann, U., Huber, R., Stone, S. R., and Hofsteenge, J. (1989) The refined 1.9 Å crystal structure of human α-thrombin: interaction with D-Phe-Pro-Arg chloromethyl ketone and significance of the Tyr-Pro-Pro-Trp insertion segment, EMBO J. 8, 3467-3475.
19. Leytus, S. P., Foster, D. C., Kurachi, K., and Davie, E. W. (1986) Gene for human factor X: A blood coagulation factor whose gene organization is essentially identical with that of factor IX and protein C, Biochemistry 25, 5098-5102.
20. Rezaie, A. R., and Yang, L. (2001) Probing the molecular basis of factor Xa specificity by mutagenesis of the serpin, antithrombin, Biochim. Biophys. Acta 1528, 167-176.
21. Smirnov, M. D., and Esmon, C. T. (1994) Phosphatidylethanolamine incorporation into vesicles selectively enhances factor Va inactivation by activated protein C, J. Biol. Chem. 269, 816-819.
22. Rezaie, A. R. (2000) Identification of basic residues in the heparin-binding exosite of factor Xa critical for heparin and factor Va binding, J. Biol. Chem. 275, 3320-3327.
23. + and factor Va in the prothrombinase complex, J. Biol. Chem. 279, 48262-48269.
24. Rezaie, A. R., and He, X. (2000) Sodium binding site of factor Xa: Role of sodium in the prothrombinase complex, Biochemistry 39, 1817-1825.
25. 2+ site in the protease domain of human coagulation factor Xa, J. Biol. Chem. 275, 36876-36884.
26. Camire, R. M. (2002) Prothrombinase assembly and S1 site occupancy restore the catalytic activity of FXa impaired by mutation at the sodium-binding site, J. Biol. Chem. 277, 37863-37870.
27. Krishnaswamy, S., and Mann, K. G. (1988) The binding of factor Va to phospholipid vesicles, J. Biol. Chem. 263, 5714-5723.
28. Nesheim, M. E., Eid, S., and Mann, K. G. (1981) Assembly of the prothrombinase complex in the absence of prothrombin, J. Biol. Chem. 256, 9874-9882.
29. +-induced allosteric regulation of catalytic activity in serine proteases, Proc. Natl. Acad. Sci. U.S.A. 93, 10653-10656.
30. Rudolph, A. E., Porche-Sorbet, R., and Miletich, J. P. (2001) Definition of a factor Va binding site in factor Xa, J. Biol. Chem. 276, 5123-5128.