Up-regulation of glutathione biosynthesis in NIH3T3 cells transformed with the ETV6-NTRK3 gene fusion

Molecules and Cells

Volumn 19, Issue 1, 2005, Pages 131-136

  Kim, Su Jung ^a   Kim, Hong Gyum ^a   Lim, Hye Won ^a   Park, Eun Hee ^b   Lim, Chang Jin ^a  

^a Kangwon National University   (South Korea)

^b Sookmyung Women's University   (South Korea)

Author keywords

Akt; ETV6 NTRK3; Glutathione; Mek1 2; NIH3T3; Up Regulation

Indexed keywords

BUTHIONINE SULFOXIMINE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; PROTEIN ETV6; PROTEIN KINASE B; PROTEIN NTRK3; TRANSCRIPTION FACTOR; TYROSINE KINASE RECEPTOR; UNCLASSIFIED DRUG; DNA BINDING PROTEIN; GLUTAMATE CYSTEINE LIGASE; GLUTATHIONE SYNTHASE; MALEIC ACID DERIVATIVE; MALEIC ACID DIETHYL ESTER; NEUROTROPHIN 3 RECEPTOR; NUCLEAR PROTEIN; REPRESSOR PROTEIN; TRANSCRIPTION FACTOR ETS; TRANSCRIPTION FACTOR ETV6;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL STRAIN 3T3; CELL TRANSFORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; FIBROBLAST; GENE FUSION; GLUTATHIONE METABOLISM; MOUSE; NONHUMAN; UPREGULATION; ANIMAL; BIOSYNTHESIS; FIBROSARCOMA; GENE TRANSLOCATION; GENETICS; METABOLISM; VIRUS CELL TRANSFORMATION;

ANIMALIA;

ANIMALS; ARTIFICIAL GENE FUSION; BUTHIONINE SULFOXIMINE; CELL TRANSFORMATION, VIRAL; DNA-BINDING PROTEINS; FIBROSARCOMA; GLUTAMATE-CYSTEINE LIGASE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE SYNTHASE; MALEATES; MICE; NIH 3T3 CELLS; NUCLEAR PROTEINS; PROTO-ONCOGENE PROTEINS C-ETS; RECEPTOR, TRKC; REPRESSOR PROTEINS; TRANSLOCATION, GENETIC; UP-REGULATION;

EID: 22844432950     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Adachi, T., Pimentel, D. R., Heibeck, T., Hou, S., Lee, Y. J., et al. (2004) S-Glutathiolation of Ras mediates redox-sensitive signaling by angiotensin II in vascular smooth muscle cells. J. Biol. Chem. 279, 29857-29862.
2. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Carretero, J., Obrador, E., Anasagasti, M. J., Martin, J. J., Vidal-Vanaclocha, F., et al. (1999) Growth-associated changes in glutathione content correlate with liver metastatic activity of B16 melanoma cells. Clin. Exp. Metastasis 17, 567-574.
4. Chuang, J. I., Chang, T. Y., and Liu, H. S. (2003) Glutathione depletion-induced apoptosis of Ha-ras-transformed NIH3T3 cells can be prevented by melatonin. Oncogene 22, 1349-1357.
5. Dolphin, D., Poulson, R., and Avromoric, O. (1989) Coenzymes and cofactors. Glutathione: Chemical, Biochemical and Medical Aspects, Vol. 3, Part A, New York.
6. Domenicotti, C., Marengo, B., Verzola, D., Garibotto, G., Traverse, N., et al. (2003) Role of PKC-delta activity in glutathione-depleted neuroblastoma cells. Free Radic. Biol. Med. 5, 504-516.
7. Ebadi, M., Bashir, R. M., Heidrick, M. L., Hamada, F. M., Refaey, H. E., et al. (1997) Neurotrophins and their receptors in nerve injury and repair. Neurochem. Int. 30, 347-374.
8. Edel, M. J. (1998) The ETS-related factor TEL is regulated by angiogenic growth factor VEGF in HUVE-cells. Anticancer Res. 18, 4505-4509.
9. Enoiu, M., Aberkane, H., Salazar, J.-F., Leroy, P., Groffen, J., et al. (2000) Evidence for the pro-oxidant effect of γ-glutamyl-transpeptidase- related enzyme. Free Radic. Biol. Med. 29, 825-833.
10. Flohé, L. and Günzler, W. A. (1984) Assay of Glutaredoxin peroxidase. Methods Enzymol. 105, 114-121.
11. Galloway, D. C., Blake, D. G., and McLellan, L. I. (1999) Regulation of γ-glutamylcysteine synthetase regulatory subunit (GLCLR) gene expression: identification of the major tranv start site in HT29 cells. Biochem. Biophys. Acta 1446, 47-56.
12. Huang, Z.-Z., Chen, C., Zeng, Z., Yang, H., Oh, J., et al. (2001) Mechanism and significance of increased glutathione level in human hepatocellular carcinoma and liver regeneration. FASEB J. 15, 19-21.
13. Iantomasi, T., Favilli, F., Degl'Innocenti, D., and Vincenzini, M. T. (1999) Increased glutathione synthesis associated with platelet-derived growth factor stimulation of NIH3T3 fibroblasts. Biochim. Biophys. Acta 1452, 303-312.
14. Ichaso, N., Rodriguez, R. E., Martin-Zanca, D., and Gonzalez-Sarmiento, R. (1998) Genomic characterization of the human trkC gene. Oncogene 17, 1871-1875.
15. Irani, K., Xia, Y., Zweier, J. L., Sollott, S. J., Der, C. J., et al. (1997) Mitogenic signaling mediated by oxidants in Rastransformed fibroblast. Science 275, 1649-1652.
16. Joneson, T. and Bar-Sagi, D. (1998) A Racl effector site controlling mitogenesis through Superoxide production. J. Biol Chem. 213, 17991-17994.
17. Knezevich, S. R., McFadden, D. E., Tao, W., Lim, J. F., and Sorensen, P. H. (1998) A novel ETV6-NTRK3 gene fusion in congenital fibrosarcoma. Nat. Genet. 18, 184-187.
18. Kuo, M. L., Chou, Y. W., Chau, Y. P., and Meng, T. C. (1996) Differential induction of apoptosis in oncogene-transformed NIH 3T3 cells by methylmethanesulfonate. Biochem. Pharwacol. 52, 481-488.
19. Lannon, C. L., Martin, M. J., Tognon, C. E., Jin, W., Kim, S. J., et al (2004) A highly conserved NTRK3 C-terminal sequence in the ETV6-NTRK3 oncoprotein binds the phosphotyrosine binding domain of insulin receptor substrate-1: an essential interaction for transformation. J. Biol. Chem. 279, 6225-6234.
20. Lee, Y-Y., Kim, H.-G., Jung, H.-I., Shin, Y H., Hong, S. M., et al. (2002) Activities of antioxidant and redox enzymes in human normal hepatic and hepatoma cell lines. Mol. Cells 14, 305-311.
21. Mates, J. M., Perez-Gomez, C., and Nunez de Castro, I. (1999) Antioxidant enzymes and human diseases. Clin. Biochem. 32, 595-603.
22. Meister, A. (1985) Glutathione synthetase from rat kidney. Methods Enzymol 113, 393-399.
23. Morrison, K. B., Tognon, C. E., Garnett, M. J., Deal, C., and Sorensen, P. H. (2002) ETV6-NTRK3 transformation requires insulin-like growth factor 1 receptor signaling and is associated with constitutive IRS-I tyrosine phosphorylation. Oncogene 21, 5684-5695.
24. Nakagawa, K., Saito, N., Tsuchida, S., Sakai, M., Tsunokawa, Y, et al. (1990) Glutathione S-transferase II as a determinant of drug resistance in transfectant cell lines. J. Biol. Chem. 265, 4296-4301.
25. Pear, W. S., Nolan, G P., Scott, M. L., and Baltimore, D. (1993) Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA 90, 8392-8396.
26. Rabin, S. J., Cleghon, V., and Kaplan, D. R. (1993) SNT, a differentiation-specific target of neurotrophic factor-induced tyrosine kinase activity in neurons and PC 12 cells. Mol. Cell. Biol. 13, 2203-2213.
27. Schnelldorfer, T., Gansauge, S., Gansauge, F., Schlosser, S., Beger, H. G., et al. (2000) Glutathione depletion causes cell growth inhibition and enhanced apoptosis in pancreatic cancer cells. Cancer 89, 1440-1447.
28. Seelig, G. F. and Meister, A. (1985) Glutathione biosynthesis; γ-glutamylcysteine synthetase from rat kidney. Methods Enzymol. 113, 379-390.
29. Seyfried, J., Soldner, F., Schulz, J. B., Klockgether, T., Kovar, K. A., et al. (1999) Differential effects of L-buthionine sulfoximine and ethacrynic acid on glutathione levels and mitochondrial function in PC12 cells. Neurosci. Lett. 264, 1-4.
30. Terradez, P., Asensi, M., Lasso De la Vega, M. C., Puertes, I. R., Vina, J., et al. (1993) Depletion of tumour glutathione in vivo by buthionine sulfoximine: modulation by the rate of cellular proliferation and inhibition of cancer growth. Biochem. J. 292, 477-483.
31. Tognon, C., Garnett, M., Kenward, E., Kay, R., Morrison, K., et al. (2001) The chimeric protein tyrosine kinase ETV6NTRK3 requires both Ras-Erk1/2 and PI3-kinase-Akt signaling for fibroblast transformation. Cancer Res. 61, 8909-8916.
32. Ushio-Fukai, M., Alexander, R. W., Akers, M., and Griendling, K. K. (1998) p38 mitogen-activated protein kinase is a critical component of the redox-sensitive signaling pathways activated by angiotensin II. Role in vascular smooth muscle cell hypertrophy. J. Biol. Chem. 273, 15022-15029.
33. Ushio-Fukai, M., Alexander, R. W., Akers, M., Yin, Q., Walsh, K., et al. (1999) Reactive oxygen species mediate the activation of Akt/protein kinase B by angiotensin II in vascular smooth muscle cells. J. Biol. Chem. 274, 22699-22704.
34. Vincenzini, M. T., Marraccini, P., Iantomasi, T., Favilli, F., Pacini, S., et al. (1993) Altered metabolism of glutathione in cells transformed by oncogenes which cause resistance to ionizing radiations. FEBS Lett. 320, 219-223.
35. Wai, D. H., Knezevich, S. R., Lucas, T., Jansen, B., Kay, R. J., et al (2000) The ETV6-NTRK3 gene fusion encodes a chi-meric protein tyrosine kinase that transforms NIH3T3 cells. Oncogene 19, 906-915.
36. Wang, L. C., Swat, W., Fujiwara, Y., Davidson, L., Visvader, J., et al. (1998) The TEL/ETV6 gene is required specifically for hematopoiesis in the bone marrow. Genes Dev. 12, 2392-2402.
37. Yang, C. F., Shen, H. M., and Ong, C. N. (1999) Protective effect of ebselen against hydrogen peroxide-induced cytotoxicity and DNA damage in HepG2 cells. Biochem. Pharmacol. 57, 273-279.