Studying the intracellular dissociation of polymer-oligonucleotide complexes by dual color fluorescence fluctuation spectroscopy and confocal imaging

Biochemistry

Volumn 44, Issue 29, 2005, Pages 9905-9912

  Lucas, B ^a   Remaut, K ^a   Sanders, N N ^a   Braeckmans, K ^a   De Smedt, S C ^a   Demeester, J ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; COLOR; DISSOCIATION; DNA; FLUORESCENCE; IMAGE ANALYSIS;

INTRACELLULAR DISSOCIATION; OLIGONUCLEOTIDES; PLASMID DNA; TRANSFECTION;

POLYMERS;

LIPOSOME; MACROGOL; OLIGONUCLEOTIDE; PEGYLATED POLY[2 (DIMETHYLAMINO)ETHYL]METHACRYLATE CO AMINOETHYL METHACRYLATE; PLASMID DNA; POLYLYSINE; POLYMER; RHODAMINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CONFOCAL LASER MICROSCOPY; DISSOCIATION; DUAL COLOR FLUORESCENCE FLUCTUATION SPECTROSCOPY; ENDOCYTOSIS; FLUORESCENCE SPECTROSCOPY; GENE DELIVERY SYSTEM; GENETIC TRANSFECTION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; VERO CELL;

ANIMALS; BUFFERS; CERCOPITHECUS AETHIOPS; CYSTEINE; CYTOPLASM; ESTERS; FLUORESCENT DYES; INTRACELLULAR FLUID; MICROINJECTIONS; MICROSCOPY, CONFOCAL; NANOTUBES; OLIGONUCLEOTIDES; POLYAMINES; POLYHYDROXYETHYL METHACRYLATE; POLYLYSINE; POLYMERS; RHODAMINES; SPECTROMETRY, FLUORESCENCE; TRANSFECTION; VERO CELLS;

EID: 22744456996     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0476883     Document Type: Article

References (33)

1. Stein, C. A., and Cheng, Y. C. (1993) Antisense oligonucleotides as therapeutic agents - Is the bullet really magical?, Science 261, 1004-1012.
2. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells, Nature 411, 494-498.
3. Harborth, J., Elbashir, S. M., Vandenburgh, K., Manninga, H., Scaringe, S. A., Weber, K., and Tuschl, T. (2003) Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing, Antisense Nucleic Acid Drug Dev. 13, 83-105.
4. Miyagishi, M., Hayashi, M., and Taira, K. (2003) Comparison of the suppressive effects of antisense oligonucleotides and siRNAs directed against the same targets in mammalian cells, Antisense Nucleic Acid Drug Dev. 13, 1-7.
5. Filmore, D. (2004) Assessing antisense. An NDA withdrawal signals another setback, but hope remains for next-generation drugs, Mod. Drug Discovery, 49-50 (June).
6. De Smedt, S. C., Demeester, J., and Hennink, W. E. (2000) Cationic polymer based gene delivery systems, Pharm. Res. 17, 113-126.
7. Gao, X., and Huang, L. (1995) Cationic liposome-mediated gene transfer, Gene Ther. 2, 710-722.
8. Felgner, P. L., Barenholz, Y., Behr, J. P., Cheng, S. H., Cullis, P., Huang, L., Jessee, J. A., Seymour, L., Szoka, F., Thierry, A. R., Wagner, E., and Wu, G. (1997) Nomenclature for synthetic gene delivery systems, Hum. Gene Ther. 8, 511-512.
9. Zabner, J., Fasbender, A. J., Moninger, T., Poellinger, K. A., and Welsh, M. J. (1995) Cellular and molecular barriers to gene transfer by a cationic lipid, J. Biol. Chem. 270, 18997-19007.
10. Simeoni, F., Morris, M. C., Heitz, F., and Divita, G. (2003) Insight into the mechanism of the peptide-based gene delivery system MPG: implications for delivery of siRNA into mammalian cells, Nucleic Acids Res. 31, 2717-2724.
11. Godbey, W. T., Wu, K. K., and Mikos, A. G. (1999) Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery, Proc. Natl. Acad. Sci. U.S.A. 96, 5177-5181.
12. Marcusson, E. G., Bhat, B., Manoharan, M., Bennett, C. F., and Dean, N. M. (1998) Phosphorothioate oligodeoxyribonucleotides dissociate from cationic lipids before entering the nucleus, Nucleic Acids Res. 26, 2016-2023.
13. Zelphati, O., and Szoka, F. C. (1996) Mechanism of oligonucleotide release from cationic liposomes, Proc. Natl. Acad. Sci. U.S.A. 93, 11493-11498.
14. Schwille, P. (2001) Fluorescence correlation spectroscopy and its potential for intracellular applications, Cell Biochem. Biophys. 34, 383-408.
15. Schwille, P., MeyerAlmes, F. J., and Rigler, R. (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution, Biophys. J. 72, 1878-1886.
16. Lucas, B., Van Rompaey, E., De Smedt, S. C., Demeester, J., and Van Oostveldt, P. (2002) Dual-color fluorescence fluctuation spectroscopy to study the complexation between poly-L-lysine and oligonucleotides, Macromolecules 35, 8152-8160.
17. Lucas, B., Remaut, K., Braeckmans, K., Haustraete, J., De Smedt, S. C., and Demeester, J. (2004) Studying pegylated DNA-complexes by dual color fluorescence correlation spectroscopy, Macromolecules 37, 3832-3840.
18. Van Rompaey, E., Sanders, N., De Smedt, S. C., Demeester, J., Van Craenenbroeck, E., and Engelborghs, Y. (2000) Complex formation between cationic polymethacrylates and oligonucleotides, Macromolecules 33, 8280-8288.
19. Van Rompaey, E., Engelborghs, Y., Sanders, N., De Smedt, S. C., and Demeester, J. (2001) Interactions between oligonucleotides and cationic polymers investigated by fluorescence correlation spectroscopy, Pharm. Res. 18, 928-936.
20. Van Rompaey, E., Chen, Y., Muller, J. D., Gratton, E., Van Craenenbroeck, E., Engelborghs, Y., De Smedt, S., and Demeester, J. (2001) Fluorescence fluctuation analysis for the study of interactions between oligonucleotides and polycationic polymers, Biol. Chem. 382, 379-386.
21. Snyder, S. L., and Sobocinski, P. Z. (1975) Improved 2,4,6- trinitrobenzenesulfonic acid method for determination of amines, Anal. Biochem. 64, 284-288.
22. Dijk-Wolthuis, W. N. E., van de Wetering, P., Hinrichs, W. L. J., Hofmeyer, L. J. F., Liskamp, R. M. J., Crommelin, D. J. A., and Hennink, W. E. (1999) A versatile method for the conjugation of proteins and peptides to poly[2-(dimethylamino)ethyl methacrylate], Bioconjugate Chem. 10, 687-692.
23. Schwille, P., MeyerAlmes, F. J., and Rigler, R. (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution, Biophys. J. 72, 1878-1886.
24. Hess, S. T., Huang, S. H., Heikal, A. A., and Webb, W. W. (2002) Biological and chemical applications of fluorescence correlation spectroscopy: A review, Biochemistry 41, 697-705.
25. Clarenc, J. P., Lebleu, B., and Leonetti, J. P. (1993) Characterization of the nuclear-binding sites of oligodeoxyribonucleotides and their analogs, J. Biol. Chem. 268, 5600-5604.
26. Hartig, R., Shoeman, R. L., Janetzko, A., Grub, S., and Traub, P. (1998) Active nuclear import of single-stranded oligonucleotides and their complexes with nonkaryophilic macromolecules, Biol. Cell 90, 407-426.
27. Lorenz, P., Baker, B. F., Bennett, C. F., and Spector, D. L. (1998) Phosphorothioate antisense oligonucleotides induce the formation of nuclear bodies, Mol. Biol. Cell 9, 1007-1023.
28. Tsuji, A., Koshimoto, H., Sato, Y., Hirano, M., Sei-Iida, Y., Kondo, S., and Ishibashi, K. (2000) Direct observation of specific messenger RNA in a single living cell under a fluorescence microscope, Biophys. J. 78, 3260-3274.
29. Shi, F. X., Visser, W. H., de Jong, N. M. J., Liem, R. S. B., Ronken, E., and Hoekstra, D. (2003) Antisense oligonucleotides reach mRNA targets via the RNA matrix: downregulation of the 5-HT1A receptor, Exp. Cell Res. 291, 313-325.
30. Liu, G., Li, D. S., Pasumarthy, M. K., Kowalczyk, T. H., Gedeon, C. R., Hyatt, S. L., Payne, J. M., Miller, T. J., Brunovskis, P., Fink, T. L., Muhammad, O., Moen, R. C., Hanson, R. W., and Cooper, M. J. (2003) Nanoparticles of compacted DNA transfect postmitotic cells, J. Biol. Chem. 278, 32578-32586.
31. Lucas, B., Van Rompaey, E., Remaut, K., Sanders, N., De Smedt, S. C., and Demeester, J. (2004) On the biological activity of anti-ICAM-1 oligonucleotides complexed to nonviral carriers, J. Controlled Release 96, 207-219.
32. Clamme, J. P., Krishnamoorthy, G., and Mély, Y. (2003) Intracellular dynamics of the gene delivery vehicle polyethylenimine during transfection: investigation by two-photon fluorescence correlation spectroscopy, Biochim. Biophys. Acta 1617, 52-61.
33. Jensen, K. D., Nori, A., Tijerina, M., Kopeckova, P., and Kopecek, J. (2003) Cytoplasmic delivery and nuclear targeting of synthetic macromolecules, J. Controlled Release 87, 89-105.