Cloning and expression of isocitrate lyase, a key enzyme of the glyoxylate cycle, of Candida albicans for development of antifungal drugs

Journal of Microbiology and Biotechnology

Volumn 15, Issue 3, 2005, Pages 652-655

  Shin, Dong Sun ^a   Kim, Sanghee ^a   Yang, Hyeong Cheol ^a   Oh, Ki Bong ^a  

^a Seoul National University   (South Korea)

Author keywords

Candida albicans; Gene expression; Glyoxylate cycle; In vitro assay; Inhibitors; Isocitrate lyase

Indexed keywords

2,5 DICHLOROBENZOQUINONE; 3 HYDROPHENYLGLYCINE; 3 NITROPROPIONIC ACID; AGAR; ANTIFUNGAL AGENT; CARBON; CITRIC ACID; DNA FRAGMENT; GLYCARBYLAMIDE; GLYCOLIC ACID; GLYOXYLIC ACID; HYDROXYECTONINE; ISOASCORBIC ACID; ISOCITRATE LYASE; ITACONIC ACID; ITACONIC ANHYDRIDE; LYASE INHIBITOR; MALIC ACID; MALONIC ACID; METAL CHELATE; OXALIC ACID; PYROGLUTAMIC ACID; QUISQUALIC ACID; SUCCINIC ACID; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANTIFUNGAL ACTIVITY; ARTICLE; CANDIDA ALBICANS; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG SYNTHESIS; DRUG TARGETING; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME REGULATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FUNGUS GROWTH; GENE AMPLIFICATION; GROWTH INHIBITION; IC 50; INCUBATION TIME; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION;

CANDIDA ALBICANS; ESCHERICHIA COLI;

EID: 22544470002     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Bishai, W. 2000. Microbiology: Lipid lunch for persistent pathogen. Nature 406: 683-685.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
3. Britton, K., S. Langridge, P. J. Baker, K. Weeradechapon, S. E. Sedelnikova, J. R. De Lucas, D. W. Rice, and G. Turner. 2000. The crystal structure and active site location of isocitrate lyase from the fungus Aspergillus nidulans. Structure Fold Des. 8: 349-362.
4. Chiou, C. C., A. H. Groll, and T. J. Walsh. 2000. New drugs and novel targets for treatment of invasive fungal infections in patients with cancer. Oncologist 5: 120-135.
5. Daniel, M. B., M. D. Rozycki, and S. J. Edelstein. 1996. Protein Methods. 2nd Ed. Wiley-Liss, New York, U.S.A.
6. Dixon, G. H. and H. L. Kornberg. 1959. Assay methods for key enzymes of the glyoxylate. J. Biochem. 72: 3P.
7. Hautzel, R., H. Anke, and W. S. Sheldrick. 1990. Myceon, a new metabolite from Mycena species TA 87202 (Basidiomycetes) as an inhibitor of isocitrate lyase. J. Antibiotics 43: 1240-1244.
8. Honer Zu Bentrup, K., A. Miczak, D. L. Swenson, and D. G. Russell. 1999. Characterization of activity and expression of isocitrate in Mycobacterium avium and Mycobacterium tuberculosis. J. Bacteriol. 181: 7161-7167.
9. Jiang, B., H. Bussey, and T. Roemer. 2002. Novel strategies in antifungal lead discovery. Curr. Opin. Microbiol. 5: 466-471.
10. Kanai, T., H. Atomi, K. Umemura, H. Ueno, Y. Teranishi, M. Ueda, and A. Tanaka. 1996. A novel heterologous gene expression system in Saccharomyces cerevisiae using the isocitrate lyase gene promoter from Candida tropicalis. Appl. Microbiol. Biotechnol. 44: 759-765.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
12. Lee, K. Y., D. S. Shin, J. M. Yoon, H. Kang, and K. B. Oh. 2002. Expression of sortase, a transpeptidase for cell wall sorting reaction, from Staphylococcus aureus ATCC 6538p in Escherichia coli. J. Microbiol. Biotechnol. 12: 530-533.
13. Lorenz, M. C. and G. R. Fink. 2001. The glyoxylate cycle is required for fungal virulence. Nature 412: 83-86.
14. Ogden, S., D. Haggerty, C. M. Stoner, D. Kolodrubetz, and R. Schleif. 1980. The Escherichia coli L-arabinose operon: Binding sites of the regulatory proteins and a mechanism of positive and negative regulation. Proc. Natl. Acad. Sci. USA 77: 3346-3350.
15. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A.
16. Sanglard, D., K. Kuchler, F. Ischer, J. L. Pagani, M. Monod, and J. Bille. 1995. Mechanisms of resistance to azole antifungal agents in Candida albicans isolates from AIDS patients involve specific multidrug transporters. Antimicrob. Agents Chemother. 39: 2378-2386.
17. Schleif, R. S. 1992. DNA looping. Annu. Rev. Biochem. 61: 199-223.
18. Sharma, V., S. Sharma, K. Hoener zu Bentrup, J. D. McKinney, D. G. Russell, W. R. Jr. Jacobs, and J. C. Sacchettini. 2000. Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis. Nat. Struct. Biol. 7: 663-668.
19. Soh, B. S., P. Loke, and T. S. Sim. 2001. Cloning, heterologous expression and purification of an isocitrate lyase from Streptomyces clavulingerus NRRL 3585. Biochim. Biophys. Acta 1552: 112-117.
20. Vanni, P., E. Giachetti, G. Pinzauti, and B. A. McFadden. 1990. Comparative structure, function and regulation of isocitrate lyase, an important assimilatory enzyme. Comp. Biochem. Physiol. 95B: 431-458.