Expression of sortase, a transpeptidase for cell wall sorting reaction, from Staphylococcus aureus ATCC 6538p in Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 12, Issue 3, 2002, Pages 530-533

  Lee, Ki Young ^a   Shin, Dong Sun ^a   Yoon, Jung Min ^a   Kang, Heonjoong ^a   Oh, Ki Bong ^a  

^a Seoul National University   (South Korea)

Author keywords

Expression; In vitro assay; Sortase gene; Staphylococcus aureus

Indexed keywords

ANTIBIOTIC AGENT; CELL SURFACE PROTEIN; ENZYME INHIBITOR; GAMMA GLUTAMYLTRANSFERASE; HISTIDINE; METAL CHELATE; SORTASE; THIOREDOXIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL WALL; CELL SELECTION; CLONING VECTOR; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC CODE; GRAM POSITIVE BACTERIUM; IN VITRO STUDY; NONHUMAN; PROTEIN DEGRADATION; STAPHYLOCOCCUS AUREUS;

ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

EID: 0036063848     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Expression of heat shock protein 70 in umbilical vein endothelial cells infected by Staphylococcus aureus
2. Sortase, a universal target for therapeutic agents against Gram-positive bacteria?
3. Antimicrobial-drug resistance
4. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin
5. Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
6. Comparison of different PCR-based genotyping techniques for MRSA discrimination among methicillin-resistant Staphylococcus aureus isolates
7. Catalyzing camouflage
8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
9. Novel fluorogenic substrates for assaying protease by resonance energy transfer
10. Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
11. Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections
12. The Escherichia coli 1-arabinose operon: Binding sites of the regulatory proteins and a mechanism of positive and negative regulation
13. DNA looping
14. The development of vancomycin resistance in a patient with methicillin-resistant Staphylococcus aureus infection
15. Anchor structure of staphylococcal surface proteins. (IV. Inhibitors of the cell wall sorting reaction)
16. Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif
17. Expression of the gene encoding protein A in Staphylococcus aureus and coagulase-negative Staphylococci
18. Design and synthesis of new fluorogenic HIV protease substrates based on resonance energy transfer
19. Surface proteins of Gram-positive bacteria and mechanisms of their targeting to the cell wall envelope
20. Use of a fluorescence plate reader for measuring kinetic parameters with inner filter effect correction