Effect of static growth and different levels of environmental oxygen toxA and ptxR expression in the Pseudomonas aeruginosa strain PAO1

Microbiology

Volumn 151, Issue 7, 2005, Pages 2263-2275

  Gaines, Jennifer M ^a   Carty, Nancy L ^a   Colmer Hamood, Jane A ^a   Hamood, Abdul N ^a  

^a TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; DISSOLVED OXYGEN; IRON; OXYGEN; PROTEIN PTXR; PSEUDOMONAS EXOTOXIN; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

AEROBIC CAPACITY; ANAEROBIC CAPACITY; ANALYSIS OF VARIANCE; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; ENVIRONMENTAL FACTOR; ENZYME IMMUNOASSAY; GROWTH CURVE; NONHUMAN; OXYGEN TENSION; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA;

ADP RIBOSE TRANSFERASES; AEROBIOSIS; ANAEROBIOSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; EXOTOXINS; GENE EXPRESSION REGULATION, BACTERIAL; OXYGEN; PSEUDOMONAS AERUGINOSA; TRANSCRIPTION FACTORS; VIRULENCE FACTORS;

PSEUDOMONAS AERUGINOSA;

EID: 22144479729     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.27754-0     Document Type: Article

References (70)

1. Bailey, J. & Manoil, C. (2002). Genome-wide internal tagging of bacterial exported proteins. Nat Biotechnol 20, 839-842.
2. Baltch, A. L. (1994). Pseudomonas bacteremia. In Pseudomonas aeruginosa Infections and Treatments, pp. 73-128. Edited by R. P. Smith & A. L. Baltch. New York: Marcel Dekker.
3. Barton, H. A., Johnson, Z., Cox, C. D., Vasil, A. I. & Vasil, M. L. (1996). Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alternations in the iron-dependent repression of exotoxin A and siderophore in aerobic and microaerobic environments. Mol Microbiol 21, 1001-1017.
4. Beare, P. A., For, R. J., Martin, L. W. & Lamont, I. L. (2003). Siderophore-mediated cell signalling in Pseudomonas aeruginosa: divergent pathways regulate virulence factor production and siderophore receptor synthesis. Mol Microbiol 47, 195-207.
5. Coligan, J. E., Kruisbeek, A. M., Margulies, D. H., Shevach, E. M. & Strober, W. (2001). Current Protocols in Immunology. Edited by R. Coico. New York: Wiley.
6. Colmer, J. A. & Hamood, A. N. (1998). Characterization of ptxS, a Pseudomonas aeruginosa gene which interferes with the effect of the exotoxin A positive regulatory gene, ptxR. Mol Gen Genet 258, 250-259.
7. Colmer, J. A. & Hamood, A. N. (1999). Expression of ptxR and its effect on toxA and regA expression during the growth cycle of the Pseudomonas aeruginosa PAO1. Can J Microbiol 45, 1008-1016.
8. Cooper, M., Tavankar, G. R. & Williams, H. D. (2003). Regulation of expression of the cyanide-insensitive terminal oxidase in Pseudomonas aeruginosa. Microbiology 149, 1275-1284.
9. Costerton, J. W., Stewart, P. S. & Greenberg, E. P. (1999). Bacterial biofilms: a common cause of persistent infections. Science 284, 1318-1322.
10. Cunliffe, H. E., Merriman, T. R. & Lamont, I. L. (1995). Cloning and characterization of pvdS, a gene required for pyoverdine synthesis in Pseudomonas aeruginosa. PvdS is probably an alternative sigma factor. J Bacteriol 177, 2744-2750.
11. Davis, P. B., Drumm, M. & Konstan, M. W. (1996). Cystic fibrosis. Am J Respir Crit Care Med 154, 1229-1256.
12. Deziel, E., Comeau, Y. & Villemur, R. (2001). Initiation of biofilm formation by Pseudomonas aeruginosa 57RP correlates with emergence of hyperpiliated and highly adherent phenotypic variants deficient in swimming, swarming, and twitching motilities. J Bacteriol 183, 1195-1204.
13. Fogle, M. R., Griswold, J. A., Oliver, J. W. & Hamood, A. N. (2002). Anti-ETA IgG neutralizes the effects of Pseudomonas aeruginosa exotoxin A. J Surg Res 106, 86-98.
14. Frank, D. W. (1997). The exoenzyme S regulon of Pseudomonas aeruginosa. Mol Microbiol 26, 621-629.
15. Frank, D. W. & Iglewski, B. H. (1988). Kinetics of toxA and regA mRNA accumulation in Pseudomonas aeruginosa. J Bacteriol 170, 4477-4483.
16. Galimand, M., Gamper, M., Zimmermann, A. & Haas, D. (1991). Positive FNR-like control of anaerobic arginine degradation and nitrate respiration in Pseudomonas aeruginosa. J Bacteriol 173, 1598-1606.
17. Govan, J. R. & Deretic, V. (1996). Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol Rev 60, 539-576.
18. Grant, C. C. & Vasil, M. L. (1986). Analysis of transcription of the exotoxin A gene of Pseudomonas aeruginosa. J Bacteriol 168, 1112-1119.
19. Hamood, A. N. & Iglewski, B. H. (1990). Expression of the Pseudomonas aeruginosa toxA positive regulatory gene (regA) in Escherichia coli. J Bacteriol 172, 589-594.
20. Hamood, A. N., Griswold, J. A. & Duhan, C. (1996a). Production of extracellular virulence factors by Pseudomonas aeruginosa isolates obtained from tracheal, urinary tract, and wound infections. J Surg Res 61, 425-432.
21. Hamood, A. N., Colmer, J. A., Ochsner, U. A. & Vasil, M. L. (1996b). Isolation and characterization of a Pseudomonas aeruginosa gene, ptxR, which positively regulates exotoxin A production. Mol Microbiol 21, 97-110.
22. Hamood, A. N., Colmer-Hamood, J. A. & Carty, N. L. (2004). Regulation of Pseudomonas aeruginosa exotoxin A synthesis. In Pseudomonas: Virulence and Gene Regulation, pp. 389-423. Edited by J.-L. Ramos. New York: Kluwer Academic/Plenum.
23. Hassett, D. J. (1996). Anaerobic production of alginate by Pseudomonas aeruginosa: alginate restricts diffusion of oxygen. J Bacteriol 178, 7322-7325.
24. Hassett, D. J., Cuppoletti, J., Trapnell, B. & 7 other authors (2002). Anaerobic metabolism and quorum sensing by Pseudomonas aeruginosa biofilms in chronically infected cystic fibrosis airways: rethinking antibiotic treatment strategies and drug targets. Adv Drug Delivery Rev 54, 1425-1443.
25. 2 tension on microbicidal function of leukocytes in wounds and in vitro. Surg Forum 27, 18-20.
26. Holder, I. (1993). Pseudomonas aeruginosa burn infections: pathogenesis and treatment. In Pseudomonas aeruginosa as an Opportunistic Pathogen, pp. 275-295. Edited by M. Campa, M. Bendinelli & H. Friedman. New York: Plenum.
27. Holloway, B. W., Krishnapillai, V. & Morgan, A. F. (1979). Chromosomal genetics of Pseudomonas. Microbiol Rev 43, 2907-2929.
28. Hollsing, A. E., Granstrom, M., Vasil, M. L., Wretlind, B. & Strandvik, B. (1987). Prospective study of serum antibodies to Pseudomonas aeruginosa exoproteins in cystic fibrosis. J Clin Microbiol 25, 1868-1874.
29. Iglewski, B. H. & Kabat, D. (1975). NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin. Proc Natl Acad Sci U S A 72, 2284-2288.
30. Jacobs, M. A., Alwood, A., Thaipisuttikul, I., Spencer, D., Haugen, E., Ernst, S., Will, O., Olson, M. V. & Manoil, C. (2003). Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 100, 14339-14344.
31. Jagger, K. S., Robinson, D. L., Franz, M. N. & Warren, R. L. (1982). Detection by enzyme-linked immunosorbent assays of antibody specific for Pseudomonas proteases and exotoxin A in sera from cystic fibrosis patients. J Clin Microbiol 15, 1054-1058.
32. Jessen, C. (2001). Temperature Regulation in Humans and Other Mammals. Berlin: Springer.
33. Jiang, C., Finkbeiner, W. E., Widdicombe, J. H., McCray, P. B., Jr & Miller, S. S. (1993). Altered fluid transport across airway epithelium in cystic fibrosis. Science 262, 424-427.
34. Kim, E. J., Sabra, W. & Zeng, A. P. (2003). Iron deficiency leads to inhibition of oxygen transfer and enhanced formation of virulence factors in cultures of Pseudomonas aeruginosa PAO1. Microbiology 149, 2627-2634.
35. Linnane, S. J., Keatings, V. M., Costello, C. M., Moynihan, J. B., O'Connor, C. M., Fitzgerald, M. X. & McLoughlin, P. (1998). Total sputum nitrate plus nitrite is raised during acute pulmonary infection in cystic fibrosis. J Resp Crit Care Med 158, 207-212.
36. Liu, P. V. (1973). Exotoxins of Pseudomonas aeruginosa. I. Factors that influence the production of exotoxin A. J Infect Dis 128, 506-513.
37. Lory, S. (1986). Effect of iron on accumulation of exotoxin A specific mRNA in Pseudomonas aeruginosa. J Bacteriol 168, 1451-1456.
38. Matsumoto, T., Furuya, N., Tateda, K., Miyazaki, S., Ohno, A., Ishii, Y., Hirakata, Y. & Yamaguchi, K. (1999). Effect of passive immunotherapy on murine gut-derived sepsis caused by Pseudomonas aeruginosa. J Med Microbiol 48, 765-770.
39. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
40. Moss, R. B., Hsu, Y. P., Lewiston, N. J., Curd, J. G., Milgrom, H., Hart, S., Dyer, B. & Larrick, J. W. (1986). Association of systemic immune complexes, complement activation, and antibodies to Pseudomonas aeruginosa lipopolysaccharide and exotoxin A with mortality in cystic fibrosis. Am Rev Resp Dis 133, 648-652.
41. Nicas, T. I. & Iglewski, B. H. (1985). The contribution of exoproducts to virulence of Pseudomonas aeruginosa. Can J Microbiol 31, 387-392.
42. Ochsner, U. A., Vasil, A. I. & Vasil, M. L. (1995). Role of the ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophore and exotoxin A expression: purification and activity of iron-regulated promoters. J Bacteriol 177, 7194-7201.
43. Ochsner, U. A., Johnson, Z., Lamont, I. L., Cunliffe, H. E. & Vasil, M. L. (1996). Exotoxin A production in Pseudomonas aeruginosa requires the iron-regulated pvdS gene encoding an alternative sigma factor. Mol Microbiol 21, 1019-1028.
44. Ohman, D. E., Sadoff, J. C. & Iglewski, B. H. (1980). Toxin A-deficient mutants of Pseudomonas aeruginosa PA103: isolation and characterization. Infect Immun 28, 899-908.
45. Pollack, M. (2000). Pseudomonas aeruginosa. In Mandell, Douglas and Bennett's Principles and Practice of Infectious Diseases. Edited by G. L. Mandell, J. E. Bennett & R. Dolin. Philadelphia, PA: Churchill Livingstone.
46. Pollack, M., Callahan, L. T., III & Taylor, N. S. (1976). Neutralizing antibody to Pseudomonas aeruginosa exotoxin in human sera: evidence for in vivo toxin production during infection. Infect Immun 14, 942-947.
47. Rahme, L. G., Stevens, E. J., Wolfort, S. F., Shao, J., Tompkins, R. G. & Ausubel, F. M. (1995). Common virulence factors for bacterial pathogenicity in plants and animals. Science 268, 1899-1902.
48. Raivio, T. L., Ujack, E. E., Rabin, H. R. & Storey, D. G. (1994). Association between transcript levels of the Pseudomonas aeruginosa regA, regB, and toxA genes in sputa of cystic fibrosis patients. Infect Immun 62, 3506-3514.
49. Raivio, T. L., Hoffer, D., Prince, R. W., Vasil, M. L. & Storey, D. G. (1996). Linker insertion scanning of regA, an activator of exotoxin A production in Pseudomonas aeruginosa. Mol Microbiol 22, 239-254.
50. Ray, A. & Williams, H. D. (1997). The effects of mutation of the anr gene on the aerobic respiratory chain of Pseudomonas aeruginosa. FEMS Microbiol Lett 156, 227-232.
51. Sabra, W., Kim, E. J. & Zeng, A. P. (2002). Physiological responses of Pseudomonas aeruginosa PAO1 to oxidative stress in controlled microaerobic and aerobic cultures. Microbiology 148, 3195-3202.
52. Sato, H. & Frank, D. W. (2004). ExoU is a potent intracellular phospholipase. Mol Microbiol 53, 1279-1290.
53. Sawer, G. R. (1991). Identification and molecular characterization of a transcriptional regulator from Pseudomonas aeruginosa PAO1 exhibiting structural and functional similarity to the FNR protein of Escherichia coli. Mol Microbiol 5, 1469-1481.
54. Singh, P. K., Parsek, M. R., Greenberg, E. P. & Welsh, M. J. (2002). A component of innate immunity prevents bacterial biofilm development. Nature 417, 552-555.
55. Stachel, S. E., An, G., Flores, C. & Nester, E. W. (1985). A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J 4, 891-898.
56. Storey, D. G., Frank, D. W., Farinha, M. A., Kropinski, A. M. & Iglewski, B. H. (1990). Multiple promoters control the regulation of the Pseudomonas aeruginosa regA gene. Mol Microbiol 4, 499-503.
57. Storey, D. G., Ujack, E. E., Rabin, H. R. & Mitchell, I. (1998). Pseudomonas aeruginosa lasR transcription correlates with the transcription of lasA, lasB, and toxA in chronic lung infections associated with cystic fibrosis. Infect Immun 66, 2521-2528.
58. Vasil, M. L. & Ochsner, U. A. (1999). The response of Pseudomonas aeruginosa to iron: genetics, biochemistry and virulence. Mol Microbiol 34, 399-413.
59. Vasil, M. L., Ochsner, U. A., Johnson, Z., Colmer, J. A. & Hamood, A. N. (1998). The Fur-regulated gene encoding the alternative sigma factor, PvdS, is required for the iron-dependent expression of the LysR-type regulator, PtxR, in Pseudomonas aeruginosa. J Bacteriol 180, 6784-6788.
60. West, S. E., Kaye, S. A., Hamood, A. N. & Iglewski, B. H. (1994). Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of exotoxin A. Infect Immun 62, 897-903.
61. Wick, M. J., Frank, D. W., Storey, D. G. & Iglewski, B. H. (1990). Structure, function, and regulation of Pseudomonas aeruginosa exotoxin A. Annu Rev Microbiol 44, 335-363.
62. Wilson, M. J., McMorran, B. J. & Lamont, I. L. (2001). Analysis of promoters recognized by PvdS, an extracytoplasmic-function sigma factor protein from Pseudomonas aeruginosa. J Bacteriol 183, 2151-2155.
63. Winteler, H. V. & Haas, D. (1996). The homologous regulators ANR of Pseudomonas aeruginosa and FNR of Escherichia coli have overlapping but distinct specificities for anaerobically inducible promoters. Microbiology 142, 685-693.
64. Wood, R. E., Boat, T. F. & Doershuk, C. F. (1976). Cystic fibrosis. Am Rev Respir Dis 113, 833-878.
65. Woods, D. E. & Vasil, M. L. (1994). Pathogenesis of Pseudomonas aeruginosa infections. In Pseudomonas aeruginosa Infections and Treatment, pp. 21-50. Edited by A. L. Baltch & R. P. Smith. New York: Marcel Dekker.
66. Worlitzsch, D., Tarran, R., Ulrich, M. & 12 other authors (2002). Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients. J Clin Invest 109, 317-325.
67. Wyckoff, T. J., Thomas, B., Hassett, D. J. & Wozniak, D. J. (2002). Static growth of mucoid Pseudomonas aeruginosa selects for nonmucoid variants that have acquired flagellum-dependent motility. Microbiology 148, 3423-3430.
68. Xu, K. D., Stewart P. S., Xia, F., Huang, C. T. & McFeters, G. A. (1998). Spatial physiological heterogeneity in Pseudomonas aeruginosa biofilm is determined by oxygen availability. Appl Environ Microbiol 64, 4035-4039.
69. Yoon, S. S., Hennigan, R. F., Hilliard, G. M. & 17 other authors (2002). Pseudomonas aeruginosa anaerobic respiration in biofilms: relationships to cystic fibrosis pathogenesis. Dev Cell 3, 593-603.
70. Zimmermann, A., Reimmann, C., Galimand, M. & Haas, D. (1991). Anaerobic growth and cyanide synthesis of Pseudomonas aeruginosa depend on anr, a regulatory gene homologous with fnr of Escherichia coli. Mol Microbiol 5, 1483-1490.