Quantification of DNA strand breaks and abasic sites by oxime derivatization and accelerator mass spectrometry: Application to γ-radiation and peroxynitrite

Analytical Biochemistry

Volumn 343, Issue 1, 2005, Pages 84-92

  Zhou, Xinfeng ^a   Liberman, Rosa G ^a   Skipper, Paul L ^a   Margolin, Yelena ^a   Tannenbaum, Steven R ^a   Dedon, Peter C ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Abasic sites; Accelerator mass spectrometry; DNA damage; Methoxyamine; Oxime derivatization; Strand breaks

Indexed keywords

ALDEHYDES; DEGRADATION; GAMMA RAYS; KETONES; MASS SPECTROMETRY; OXIDATION; PARTICLE ACCELERATORS; SUGAR INDUSTRY;

ABASIC SITES; ACCELERATOR MASS SPECTROMETRY; DERIVATIZATIONS; DNA DAMAGES; METHOXYAMINE; STRAND BREAKS;

DNA;

ALDEHYDE; APURINIC ACID; DEOXYRIBOSE; KETONE; METHOXYAMINE; OXIME; OXIME DERIVATIVE; PEROXYNITRITE; SUGAR;

APYRIMIDINIC SITE; ARTICLE; COMPARATIVE STUDY; CORRELATION ANALYSIS; DNA DAMAGE; DNA ISOLATION; DNA REPAIR; DNA STRAND BREAKAGE; GAMMA RADIATION; MASS SPECTROMETRY; MEASUREMENT; OXIDATION; PLASMID; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS;

EID: 22144464125     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.05.007     Document Type: Article

References (27)

1. T. Lindahl Instability and decay of the primary structure of DNA Nature 362 1993 709 714
2. P.C. Dedon, and S.R. Tannenbaum Reactive nitrogen species in the chemical biology of inflammation Arch. Biochem. Biophys. 423 2004 12 22
3. P.C. Dedon, and I.H. Goldberg Free-radical mechanisms involved in the formation of sequence-dependent bistranded DNA lesions by the antitumor antibiotics bleomycin, neocarzinostatin, and calicheamicin Chem. Res. Toxicol. 5 1992 311 332
4. W.K. Pogozelski, and T.D. Tullius Oxidative strand scission of nucleic acids: routes initiated by hydrogen atom abstraction from the sugar moiety Chem. Rev. 98 1998 1089 1107
5. A. Sancar, L.A. Lindsey-Boltz, K. Unsal-Kaccmaz, and S. Linn Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints Annu. Rev. Biochem. 73 2004 39 85
6. B. Demple, and L. Harrison Repair of oxidative damage to DNA: enzymology and biology Annu. Rev. Biochem. 63 1994 915 948
7. C. Collins, M.M. Awada, X. Zhou, and P.C. Dedon Analysis of 3′-phosphoglycolaldehyde residues in oxidized DNA by gas chromatography/negative chemical ionization/mass spectrometry Chem. Res. Toxicol. 16 2003 1560 1566
8. A.R. Collins The comet assay for DNA damage and repair: principles, applications, and limitations Mol. Biotechnol. 26 2004 249 261
9. S. Sauvaigo, C. Petec-Calin, S. Caillat, F. Odin, and J. Cadet Comet assay coupled to repair enzymes for the detection of oxidative damage to DNA induced by low doses of gamma-radiation: use of yoyo-1, low-background slides, and optimized electrophoresis conditions Anal. Biochem. 303 2002 107 109
10. K. Kubo, H. Ide, S.S. Wallace, and Y.W. Kow A novel, sensitive, and specific assay for abasic sites, the most commonly produced DNA lesion Biochemistry 31 1992 3703 3708
11. J. Nakamura, V.E. Walker, P.B. Upton, S.Y. Chiang, Y.W. Kow, and J.A. Swenberg Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions Cancer Res. 58 1998 222 225
12. M. Liuzzi, and M. Talpaert-Borle A new approach to the study of the base-excision repair pathway using methoxyamine J. Biol. Chem. 260 1985 5252 5258
13. M. Talpaert-Borle, and M. Liuzzi Reaction of apurinic/apyrimidinic sites with [14C]methoxyamine. A method for the quantitative assay of AP sites in DNA Biochim. Biophys. Acta 740 1983 410 416
14. R.G. Liberman, B.J. Hughey, P.L. Skipper, J.S. Wishnok, R.E. Klinkowstein, R.E. Shefer, and S.R. Tannenbaum The NSI biomedical AMS system at MIT: current status Nucl. Instr. Methods 223-224 2004 82 86
15. R.G. Liberman, S.R. Tannenbaum, B.J. Hughey, R.E. Shefer, R.E. Klinkowstein, C. Prakash, S.P. Harriman, and P.L. Skipper An interface for direct analysis of (14)c in nonvolatile samples by accelerator mass spectrometry Anal. Chem. 76 2004 328 334
16. W.A. Pryor, R. Cueto, X. Jin, W.H. Koppenol, M. Ngu-Schwemlein, G.L. Squadrito, P.L. Uppu, and R.M. Uppu A practical method for preparing peroxynitrite solutions of low ionic strength and free of hydrogen peroxide Free Radic. Biol. Med. 18 1995 75 83
17. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl Current Protocols in Molecular Biology 1989 John Wiley and Sons New York
18. L.F. Povirk, and C.W. Houlgrave Effect of apurinic/apyrimidinic endonucleases and polyamines on DNA treated with bleomycin and neocarzinostatin: specific formation and cleavage of closely opposed lesions in complementary strands Biochemistry 27 1988 3850 3857
19. J.R. Milligan, J.A. Aguilera, and J.F. Ward Variation of single-strand break yield with scavenger concentration for plasmid DNA irradiated in aqueous solution Radiat. Res. 133 1993 151 157
20. B.C. Blount, and B.N. Ames Analysis of uracil in DNA by gas chromatography-mass spectrometry Anal. Biochem. 219 1994 195 200
21. S. Burney, J.C. Niles, P.C. Dedon, and S.R. Tannenbaum DNA damage in deoxynucleosides and oligonucleotides treated with peroxynitrite Chem. Res. Toxicol. 12 1999 513 520
22. N.Y. Tretyakova, S. Burney, B. Pamir, J.S. Wishnok, P.C. Dedon, G.N. Wogan, and S.R. Tannenbaum Peroxynitrite-induced DNA damage in the supF gene: correlation with the mutational spectrum Mutat. Res. 447 2000 287 303
23. J.P. Radicella, C. Dherin, C. Desmaze, M.S. Fox, and S. Boiteux Cloning and characterization of hOGG1, a human homolog of the OGG1 gene of Saccharomyces cerevisiae Proc. Natl. Acad. Sci. USA 94 1997 8010 8015
24. C. Dherin, J.P. Radicella, M. Dizdaroglu, and S. Boiteux Excision of oxidatively damaged DNA bases by the human alpha-hOGG1 protein and the polymorphic alpha-hOGG1(ser326cys) protein which is frequently found in human populations Nucleic Acids Res. 27 1999 4001 4007
25. H. Atamna, I. Cheung, and B.N. Ames A method for detecting abasic sites in living cells: age-dependent changes in base excision repair Proc. Natl. Acad. Sci. USA 97 2000 686 691
26. S. Frelon, T. Douki, J.L. Ravanat, J.P. Pouget, C. Tornabene, and J. Cadet High-performance liquid chromatography-tandem mass spectrometry measurement of radiation-induced base damage to isolated and cellular DNA Chem. Res. Toxicol. 13 2000 1002 1010
27. C. Collins, X. Zhou, R. Wong, J. Coderre, and P. Dedon Differential oxidation of deoxyribose in DNA by γ- and α-radiation Rad. Res. 163 2005 654 662