Comet assay coupled to repair enzymes for the detection of oxidative damage to DNA induced by low doses of γradiation: Use of YOYO-1, low-background slides, and optimized electrophoresis conditions

Analytical Biochemistry

Volumn 303, Issue 1, 2002, Pages 107-109

  Sauvaigo, Sylvie ^a   Petec Calin, Crina ^b   Caillat, Sylvain ^a   Odin, Francette ^a   Cadet, Jean ^a  

^a CEA GRENOBLE   (France)

^b CAROL DAVILA UNIVERSITY OF MEDICINE AND PHARMACY   (Romania)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS;

COMET ASSAYS; LOW BACKGROUND; LOW DOSE; OXIDATIVE DAMAGE; REPAIR ENZYMES;

DAMAGE DETECTION;

ENZYME; ETHIDIUM BROMIDE; FLUORESCENT DYE; UNCLASSIFIED DRUG; YOYO 1;

COMET ASSAY; DNA DAMAGE; DNA REPAIR; ELECTROPHORESIS; FLUORESCENCE; GAMMA RADIATION; HUMAN; HUMAN CELL; NOTE; OXIDATIVE STRESS; PRIORITY JOURNAL; RADIATION DOSE;

EID: 0036535495     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5559     Document Type: Article

References (5)

1. Measurement of DNA base damage in cells exposed to low doses of γ-radiation: Comparison between the HPLC-EC and comet assays
2. Induction of DNA single-strand breaks in human lymphocytes by low doses of γ-rays
3. Analysis of fluoroquinolone-mediated photosensitization of 2′-deoxyguanosine, calf thymus and cellular DNA: Determination of type-I, type-II and triplet-triplet energy transfer mechanism contribution
4. Direct visualization of individual DNA molecules by fluorescence microscopy: Characterization of the factors affecting signal/background and optimization of imaging conditions using YOYO
5. Detection of DNA damage by the alkaline comet assay after exposure to low-dose gamma radiation