Expansion of cord blood CD34+ hematopoietic progenitor cells in coculture with autologous umbilical vein endothelial cells (HUVEC) is superior to cytokine-supplemented liquid culture

Bone Marrow Transplantation

Volumn 36, Issue 1, 2005, Pages 71-79

  Yildirim, S ^a   Boehmler, A M ^a   Kanz, L ^a   Möhle, Robert ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

CD34; Cord blood; Ex vivo expansion; Hematopoietic progenitor cells; HUVEC

Indexed keywords

CD34 ANTIGEN; CYTOKINE; FLT3 LIGAND; INTERLEUKIN 1; INTERLEUKIN 6; VASCULOTROPIN;

ANTIGEN EXPRESSION; ARTICLE; BONE MARROW CELL; CELL COUNT; CELL CULTURE; CELL GROWTH; CELL ISOLATION; CELL MATURATION; CLONOGENIC ASSAY; CONTROLLED STUDY; CORD BLOOD STEM CELL TRANSPLANTATION; CULTURE MEDIUM; DILUTION; ENDOTHELIUM CELL; EX VIVO STUDY; FEASIBILITY STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; LIQUID CULTURE; PRIORITY JOURNAL; STUDENT T TEST; UMBILICAL CORD BLOOD; UMBILICAL VEIN; VASCULAR ENDOTHELIUM; XENOGRAFT;

ANTIGENS, CD34; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; COCULTURE TECHNIQUES; CULTURE MEDIA; CYTOKINES; ENDOTHELIAL CELLS; ENDOTHELIUM, VASCULAR; FETAL BLOOD; HEMATOPOIETIC STEM CELLS; HUMANS; UMBILICAL VEINS;

EID: 21744443464     PISSN: 02683369     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bmt.1705001     Document Type: Article

References (31)

1. Mohle R, Rafii S, Moore MA. The role of endothelium in the regulation of hematopoietic stem cell migration. Stem Cells 1998; 16 (Suppl 1): 159-165.
2. Mohle R, Bautz F, Rafii S et al. Regulation of transendothelial migration of hematopoietic progenitor cells. Ann N Y Acad Sci 1999; 872: 176-185.
3. Bautz F, Rafii S, Kanz L et al. Expression and secretion of vascular endothelial growth factor-A by cytokine-stimulated hematopoietic progenitor cells. Possible role in the hematopoietic microenvironment. Exp Hematol 2000; 28: 700-706.
4. Piacibello W, Sanavio F, Severino A et al. Engraftment in nonobese diabetic severe combined immunodeficient mice of human CD34(+) cord blood cells after ex vivo expansion: evidence for the amplification and self-renewal of repopulating stem cells. Blood 1999; 93: 3736-3749.
5. Piacibello W, Bruno S, Sanavio F et al. Lentiviral gene transfer and ex vivo expansion of human primitive stem cells capable of primary, secondary, and tertiary multilineage repopulation in NOD/SCID mice. Blood 2002; 100: 4391-4400.
6. Guenechea G, Segovia JC, Albella B et al. Delayed engraftment of nonobese diabetic/severe combined immunodeficient mice transplanted with ex vivo-expanded human CD34(+) cord blood cells. Blood 1999; 93: 1097-1105.
7. Brandt JE, Galy AH, Luens KM et al. Bone marrow repopulation by human marrow stem cells after long-term expansion culture on a porcine endothelial cell line. Exp Hematol 1998; 26: 950-961.
8. Rafii S, Shapiro F, Pettengell R et al. Human bone marrow microvascular endothelial cells support long-term proliferation and differentiation of myeloid and megakaryocytic progenitors. Blood 1995; 86: 3353-3363.
9. Chute JP, Clark W, Saini A et al. Rescue of hematopoietic stem cells following high-dose radiation injury using ex vivo culture on endothelial monolayers. Mil Med 2002; 167: 74-77.
10. Davis TA, Robinson DH, Lee KP et al. Porcine brain microvascular endothelial cells support the in vitro expansion of human primitive hematopoietic bone marrow progenitor cells with a high replating potential: requirement for cell-to-cell interactions and colony-stimulating factors. Blood 1995; 85: 1751-1761.
11. Candal FJ, Rafii S, Parker JT et al. BMEC-1: a human bone marrow microvascular endothelial cell line with primary cell characteristics. Microvasc Res 1996; 52: 221-234.
12. Shi Q, Rafii S, Wu MH et al. Evidence for circulating bone marrow-derived endothelial cells. Blood 1998; 92: 362-367.
13. Wang JC, Doedens M, Dick JE. Primitive human hematopoietic cells are enriched in cord blood compared with adult bone marrow or mobilized peripheral blood as measured by the quantitative in vivo SCID-repopulating cell assay. Blood 1997; 89: 3919-3924.
14. Tanavde VM, Malehorn MT, Lumkul R et al. Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood. Exp Hematol 2002; 30: 816-823.
15. Jaffe EA, Nachman RL, Becker CG et al. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-2756.
16. Liu LQ, Sposato M, Liu HY et al. Functional cloning of IGFBP-3 from human microvascular endothelial cells reveals its novel role in promoting proliferation of primitive CD34+CD38-hematopoietic cells in vitro. Oncol Res 2003; 13: 359-371.
17. Schweitzer KM, Drager AM, van der Valk P et al. Constitutive expression of E-selectin and vascular cell adhesion molecule-1 on endothelial cells of hematopoietic tissues. Am J Pathol 1996; 148: 165-175.
18. Jacobsen K, Kravitz J, Kincade PW et al. Adhesion receptors on bone marrow stromal cells: in vivo expression of vascular cell adhesion molecule-1 by reticular cells and sinusoidal endothelium in normal and gamma-irradiated mice. Blood 1996; 87: 73-82.
19. Breems DA, Blokland EA, Neben S et al. Frequency analysis of human primitive haematopoietic stem cell subsets using a cobblestone area forming cell assay. Leukemia 1994; 8: 1095-1104.
20. Ploemacher RE, van der Sluijs JP, Voerman JS et al. An in vitro limiting-dilution assay of long-term repopulating hematopoietic stem cells in the mouse. Blood 1989; 74: 2755-2763.
21. Harris DT. Experience in autologous and allogeneic cord blood banking. J Hematother 1996; 5: 123-128.
22. Fruchtman SM, Hurlet A, Dracker R et al. The successful treatment of severe aplastic anemia with autologous cord blood transplantation. Biol Blood Marrow Transplant 2004; 10: 741-742.
23. Gan OI, Murdoch B, Larochelle A et al. Differential maintenance of primitive human SCID-repopulating cells, clonogenic progenitors, and long-term culture-initiating cells after incubation on human bone marrow stromal cells. Blood 1997; 90: 641-650.
24. Zsebo KM, Yuschenkoff VN, Schiffer S et al. Vascular endothelial cells and granulopoiesis: interleukin-1 stimulates release of G-CSF and GM-CSF. Blood 1988; 71: 99-103.
25. Mierke CT, Ballmaier M, Werner U et al. Human endothelial cells regulate survival and proliferation of human mast cells. J Exp Med 2000; 192: 801-811.
26. Mohle R, Green D, Moore MA et al. Constitutive production and thrombin-induced release of vascular endothelial growth factor by human megakaryocytes and platelets. Proc Natl Acad Sci USA 1997; 94: 663-668.
27. Kollet O, Moore JG, Aviram R et al. The plant lectin FRIL supports prolonged in vitro maintenance of quiescent human cord blood CD34(+)CD38(-/low)/SCID repopulating stem cells. Exp Hematol 2000; 28: 726-736.
28. Chute JP, Saini AA, Chute DJ et al. Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow. Blood 2002; 100: 4433-4439.
29. Scheding S, Franke H, Diehl V et al. How many myeloid post-progenitor cells have to be transplanted to completely abrogate neutropenia after peripheral blood progenitor cell transplantation? Results of a computer simulation. Exp Hematol 1999; 27: 956-965.
30. McNiece I, Jones R, Bearman SI et al. Ex vivo expanded peripheral blood progenitor cells provide rapid neutrophil recovery after high-dose chemotherapy in patients with breast cancer. Blood 2000; 96: 3001-3007.
31. Jaroscak J, Goltry K, Smith A et al. Augmentation of umbilical cord blood (UCB) transplantation with ex vivo-expanded UCB cells: results of a phase 1 trial using the AastromReplicell System. Blood 2003; 101: 5061-5067.