Improving the monitoring of methanol concentration during high cell density fermentation of Pichia pastoris

Biotechnology Letters

Volumn 26, Issue 18, 2004, Pages 1447-1452

  Ramon, R ^a   Feliu, J X ^b   Cos, O ^a   Montesinos, J L ^a   Berthet, F X ^b   Valero, F ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b INOVA DIAGNOSTICS INC   (United States)

Author keywords

Fermentation monitoring; On line methanol; Pichia pastoris

Indexed keywords

CALIBRATION; CONCENTRATION (PROCESS); ERROR ANALYSIS; FERMENTATION; PROTEINS; SEMICONDUCTOR DEVICES; SENSORS; VAPORS;

METHANOL CONCENTRATIONS; ON-LINE MONITORING; PICHIA PASTORIS; SEMICONDUCTOR SENSORS;

METHANOL;

PICHIA PASTORIS;

METHANOL; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; CELL COUNT; CELL PROLIFERATION; CULTURE TECHNIQUE; ENZYMOLOGY; EQUIPMENT; EQUIPMENT DESIGN; EVALUATION; FEEDBACK SYSTEM; FERMENTATION; FLOW CYTOMETRY; FLOW INJECTION ANALYSIS; GENETICS; INSTRUMENTATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; PICHIA; RHIZOPUS; TRANSDUCER;

BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; FEEDBACK; FERMENTATION; FLOW CYTOMETRY; FLOW INJECTION ANALYSIS; LIPASE; METHANOL; PICHIA; RECOMBINANT PROTEINS; RHIZOPUS; TRANSDUCERS;

EID: 21644447149     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:BILE.0000045649.60508.c5     Document Type: Article

References (9)

1. Cregg JM, Lin Cereghino J, Shi J, Higgins DR (2000) Recombinant protein expression in Pichia pastoris. Mol. Biotechnol. 16: 23-52.
2. Crowley J, McCarthy B, Nunn NS, Harvey LM, McNeil B (2000) Monitoring a recombinant Pichia pastoris fed batch process using Fourier transform mid-infrared spectroscopy (FT-MIRS). Biotechnol. Lett. 22: 1907-1912.
3. Guarna MM, Lesnicki, GJ, Tam BM, Robinson J, Radziminski CZ, Hasenwinkle D, Boraston A, Jervis E, Mac Gillvray RTA, Turner RFB, Kilburn DG (1997) On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris. Biotechnol. Bioeng. 56: 279-286.
4. Hellwig S, Emde F, Raven, NPG, Henke M, van der Logt P, Fischer R (2001) Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 74: 344-352.
5. Invitrogen Corporation, CA, USA. Pichia Expression Kit. Protein expression. A Manual of Methods for Expression of Recombinant Proteins in Pichia Pastoris. Catalog no. K1710-01. Version F.
6. Lin Cereghino J, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66.
7. Minning S, Serrano A, Ferrer P, Solà C, Schmid RD, Valero F (2001) Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotecnol. 86: 59-70.
8. Shioya S (1992) Optimization and control in fed-batch bioreactors. In: Fiechter A, eds. Advances in Biochemical/Engineering/Biotechnology. Berlin: Springer, pp. 111-142.
9. Surribas A, Cos O, Montesinos JL, Valero F (2003) On-line monitoring of the methanol concentration in Pichia pastoris cultures producing an heterologous lipase by sequential injection analysis. Biotechnol. Lett. 25: 1795-1800.