Certain site-directed, nonenzymatically active mutants of the Haemophilus influenzae P4 lipoprotein are able to elicit bactericidal antibodies

Infection and Immunity

Volumn 73, Issue 7, 2005, Pages 4454-4457

  Green, Bruce A ^a,c   Baranyi, Elizabeth ^a   Reilly, Thomas J ^b,d   Smith, Arnold L ^b,e   Zlotnick, Gary W ^a  

^a WYETH RESEARCH   (United States)

^b University of Missouri   (United States)

^c Wyeth   (United States)

^d UNIVERSITY OF MISSOURI   (United States)

^e SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIUM ANTIBODY; HAEMOPHILUS INFLUENZAE VACCINE; IMMUNOGLOBULIN G; LIPOPROTEIN; MUTANT PROTEIN; PROTEIN P4; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; HAEMOPHILUS INFLUENZAE; HEL GENE; IMMUNOGENICITY; MOUSE; NONHUMAN; OTITIS MEDIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

ANTIBODIES, BACTERIAL; BACTERIAL PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HAEMOPHILUS INFLUENZAE; HUMANS; LIPOPROTEINS; MUTATION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 21544464291     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.73.7.4454-4457.2005     Document Type: Article

References (16)

1. Bernstein, J. M., H. S. Faden, and P. L. Ogra. 1991. Nasopharyngeal colonization by nontypeable Haemophilus influenzae in children: the effect of serum bactericidal antibody. Otolaryngol. Head Neck Surg. 105:406-410.
2. Brinton, C. C., Jr., M. J. Carter, D. B. Derber, S. Kar, J. A. Kramarik, A. C. C. To, and S. W. Wood. 1989. Design and development of pilus vaccines for Haemophilus influenzae diseases. Pediatr. Infect. Dis. J. 8(Suppl.):54-61.
3. Green, B. A., J. E. Farley, T. Quinn-Dey, R. A. Deich, and G. W. Zlotnick. 1991. The e (P4) outer membrane protein of Haemophilus influenzae: biologic activity of anti-e serum and cloning and sequencing of the structural gene. Infect. Immun. 59:3191-3198.
4. Green, B. A., B. J. Metcalf, T. Quinn-Dey, D. H. Kirkley, S. A. Quataert, and R. A. Deich. 1990. A recombinant non-fatty acylated form of the Hi-PAL (P6) protein of Haemophilus influenzae elicits biologically active antibody against both nontypeable and type b H. influenzae. Infect. Immun. 58:3272-3278.
5. Green, B. A., S. B. Olmsted, M. Vazquez, G. Quigley-Reape, and G. W. Zlotnick. 1998. A clinical vaccine formulation for Haemophilus influenzae otitis media containing recombinant P4 and P6 outer membrane proteins, p. 355-362. In M. Tos, J. Thomsen, and V. Balle (ed.), Recent advances in otitis media: proceedings of the fourth extraordinary international symposium. Kugler Publications, Amsterdam, The Netherlands.
6. Hotomi, M., Y. Ikeda, M. Suzumoto, K. Yamauchi, B. A. Green, G. Zlotnick, D. S. Billal, J. Shimada, K. Fujihara, and N. Yamanaka. 2005. A recombinant P4 protein of Haemophilus influenzae induces responses biologically active against nasopharyngeal colonization after intranasal immunization. Vaccine 23:1294-1300.
7. Karasic, R., D. J. Beste, S. C.-M. To, W. J. Doyle, S. J. Wood, M. J. Carter, A. C. C. To, K. Tanpowpong, C. D. Bluestone, and C. C. Brinton, Jr. 1988. Evaluation of pilus vaccines for prevention of experimental otitis media caused by nontypable Haemophilus influenzae. Pediatr. Infect. Dis. J. 8(Suppl.):S62-S65.
8. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
9. Mason, K. W., D. Zhu, C. A. Scheuer, J. C. McMichael, G. W. Zlotnick, and B. A. Green. 2004. Reduction of nasal colonization of nontypeable Haemophilus influenzae following intranasal immunization with rLP4/rLP6/ UspA2 proteins combined with aqueous formulation of RC529. Vaccine 22:3449-3456.
10. Reidl, J., and J. J. Mekalanos. 1996. Lipoprotein e (P4) is essential for hemin uptake by Haemophilus influenzae. J. Exp. Med. 183:621-629.
11. Reilly, T., and A. Smith. 1999. Purification and characterization of a recombinant Haemophilus influenzae outer membrane phosphomonoesterase e. Protein Expr. Purif. 17:401-409.
12. Reilly, T. J., D. L. Chance, and A. L. Smith. 1999. Outer membrane lipoprotein e (P4) of Haemophilus influenzae is a novel phosphomonoesterase. J. Bacteriol. 181:6797-6805.
13. Reilly, T. J., B. A. Green, G. W. Zlotnick, and A. L. Smith. 2001. Contribution of the DDDD motif of H. influenzae e (P4) to phosphomonoesterase activity and heme transport. FEBS Lett. 494:19-23.
14. Rossolini, G. M., S. Schippa, M. L. Riccio, F. Berlutti, L. E. Macaskie, and M. C. Thaller. 1998. Bacterial nonspecific acid phosphohydrolases: physiology, evolution and use as tools in microbial biotechnology. Cell. Mol. Life Sci. 54:833-850.
15. Thaller, M. C., S. Schippa, and G. M. Rossolini. 1998. Conserved sequence motifs among bacterial, eukaryotic, and archaeal phosphatases that define a new phosphohydrolase superfamily. Protein Sci. 7:1647-1652.
16. Zagursky, R. J., P. Ooi, K. F. Jones, M. J. Fiske, R. P. Smith, and B. A. Green. 2000. Identification of a Haemophilus influenzae 5′-nucleotidase protein: cloning of the nucA gene and immunogenicity and characterization of the NucA protein. Infect. Immun. 68:2525-2534.