Identification of a Haemophilus influenzae 5'-nucleotidase protein: Cloning of the nucA gene and immunogenicity and characterization of the NucA protein

Infection and Immunity

Volumn 68, Issue 5, 2000, Pages 2525-2534

  Zagursky, Robert J ^a   Ooi, Peggy ^a   Jones, Kevin F ^a,b   Fiske, Michael J ^a   Smith, Robert P ^a   Green, Bruce A ^a  

^a PFIZER INC   (United States)

^b SIGA Technologies Incorporated   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5' NUCLEOTIDASE; BACTERIAL PROTEIN; NUCA PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERICIDAL ACTIVITY; HAEMOPHILUS INFLUENZAE; IMMUNOELECTRON MICROSCOPY; IMMUNOGENICITY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS;

5'-NUCLEOTIDASE; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS; BASE SEQUENCE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, BACTERIAL; GENE EXPRESSION; GENES, BACTERIAL; HAEMOPHILUS INFLUENZAE; MICE; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; RABBITS; RATS; RATS, SPRAGUE-DAWLEY; RECOMBINANT FUSION PROTEINS;

EID: 0034017268     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.68.5.2525-2534.2000     Document Type: Article

References (47)

1. Akkoyunlu, M., A. Melhus, C. Capiau, O. Van Opstal, and A. Forsgren. 1997. The acylated form of protein D of Haemophitus influenzae is more immunogenic than the nonacylated form and elicits an adjuvant effect when it is used as a carrier conjugated to polyribosyl ribitol phosphate. Infect. Immun. 65:5010-5016.
2. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Barbic, J., M. F. Leef, D. L. Burns, and R. D. Shahin. 1997. Role of gamma Interferon in natural clearance of Bordetella pertussis infection. Infect. Immun. 65:4904-4908.
4. Barenkamp, S. J. 1996. Immunization with high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae modifies experimental otitis media in chinchillas. Infect. Immun. 64:1246-1251.
5. Beacham, I. R., R. Kahana, L. Levy, and E. Yagil. 1973. Mutants of Escherichia coli K-12 "cryptic," or deficient in 5′-nucleotidase (uridine diphosphate-sugar hydrolase) and 3′-nucleotidase (cyclic phosphodiesterase) activity. J. Bacteriol. 116:957-964.
6. Bengis-Garber, C. 1985. Membrane-bound 5′-nucleotidase in marine luminus bacteria: biochemical and immunological properties. Can. J. Microhiol. 31:543-548.
7. Bluestone, C. D., and J. O. Klein. 1983. Otitis media with effusion, atelectasis, and eustacian tube dysfunction, p. 356. In C. D. Bluestone and S. E. Stool (ed.). Pediatric otolaryngology. The W. B. Saunders Co., Philadelphia, Pa.
8. Brinton, C. C., Jr., M. J. Carter, D. B. Derber, S. Kar, J. A. Kramarik, A. C. C. To, and S. W. Wood. 1989. Design and development of pilus vaccines for Haemophilus influenzae diseases. Pediatr. Infect. Dis. J. 8S:54-61.
9. Burns, D. M., and I. R. Beacham. 1985. Nucleotide sequence and transcriptional analysis of the E. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5′-nucleotidase): regulation of the ushA gene, and the signal sequence of its endoded protein product. Nucleic Acids Res. 14:4325-4342.
10. Burns, D. M., and I. R. Beacham. 1986. Identification and sequence analysis of a silent gene (ushA0) in Salmonella typhimurium. J. Mol. Biol. 20:163-175.
11. Chen, P. S., Jr., T. Y. Toribara, and H. Warner. 1956. Microdetermination of phosphorus. Anal. Chem. 28:1756-1758.
12. Deich, R. A., A. Anilionis, J. Fulginiti, B. J. Metcalf, S. Quataert, T. Quinn-Dey, G. W. Zlotnick, and B. A. Green. 1990. Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera. Infect. Immun. 58:3388-3393.
13. Deich, R. A., B. J. Metcalf, C. W. Finn, J. E. Farley, and B. A. Green. 1988. Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae. J. Bacteriol. 170:489-498.
14. Duplay, P., H. Dedouelle, A. Fowler, I. Zabin, W. Saurin, and M. Hofnung. 1984. Sequences of the malE gene and of its product, the maltose-binding protein of Escherichia coli K12. J. Biol. Chem. 259:10606-10613.
15. Faden, H., J. Bernstein, L. Brodsky, J. Stanievich, D. Krystofik, C. Shuff, J. J. Hong, and P. L. Ogra. 1989. Otitis media in children. I. The systemic immune response to nontypable Haemophilus influenzae. J. Infect. Dis. 160: 999-1004.
16. Fleischmann, R. D., M. D. Adams, W. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J.-F. Tomb, B. A. Dougherty, J. M. Merrick, K, McKenney, G. Sutton, W. FitzHugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L.-I. Liu, A. Glodek, J. M. Kelley, J. F. Weidman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, N. S. M. Geoghagen, C. L. Gnehn, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
17. Garcia, L., L. Chayet, A. C. Kettlun, L. Collados, M. Chiong, A. Traverso-Cori, M. Mancilla, and M. A. Valenzuela. 1997. Kinetic characteristics of nucleoside mono-, di- and triphosphatase activities of the periplasmid 5′-nucleotidase of Escherichia coli. Comp. Biochem. Physiol. 117B:135-142.
18. Green, B. A., J. E. Farley, T. Quinn-Dey, R. A. Deich, and G. W. Zlotnick. 1991. The e (P4) outer membrane protein of Haemophilus influenzae: biologic activity of anti-e serum and cloning and sequencing of the structural gene. Infect. Immun. 59:3191-3198.
19. Green, B. A., B. J. Metcalf, T. Quinn-Dey, D. H. Kirkley, S. A. Quataert, and R. A. Deich. 1990. A recombinant non-fatty acylated form of the Hi-PAL (P6) protein of Haemophilus influenzae elicits biologically active antibody against both nontypeable and type b H. influenzae. Infect. Immun. 58:3272-3278.
20. Green, B. A., T. Quinn-Dey, and G. W. Zlotnick. 1987. Biologic activities of antibody to peptidoglycan-associated lipoprotein of Haemophilus influenzae against multiple clinical isolates of H. influenzae type b. Infect. Immun. 55:1278-1284.
21. Green, B. A., M. E. Vazquez, G. W. Zlotnick, G. Quigley-Reape, J. D. Swarts, I. Green, J. L. Cowell, C. D. Bluestone, and W. J. Doyle. 1993. Evaluation of mixtures of purified Haemophilus influenzae outer membrane proteins in protection against challenge with nontypeable H. influenzae in the chinchilla otitis media model. Infect. Immun. 61:1950-1957.
22. Haase, E. M., A. A. Campagnari, J. Sarwar, M. Shero, M. Wirth, C. U. Cumming, and T. Murphy. 1991. Strain-specific and immunodominant surface epitopes of the P2 porin protein of nontypeable Haemophilus influenzae. Infect. Immun. 56:1278-1284.
23. Harabuchi, Y., H. Faden, N. Yamanaka, L. Duffy, J. Wolf, and D. Krystofik. 1994. Human milk secretory IgA antibody to nontypeable Haemophilus influenzae: possible protective effects against nasopharyngeal colonization. J. Pediatr. 124:193-198.
24. Loosmore, S. M., Y.-P. Yang, D. C. Coleman, J. M. Shortreed, D. M. England, and M. H. Klein. 1997. Outer membrane protein D15 is conserved among Haemophilus influenzae species and may represent a universal protective antigen against invasive disease. Infect. Immun. 65:3701-3707.
25. Misumi, Y., S. Ogata, S. Hirose, and Y. Ikehara. 1990. Primary structure of rat liver 5′-nucleotidase deduced from the cDNA. J. Biol. Chem. 265:2178-2183.
26. Misumi, Y., S. Ogata, K. Ohkubo, S. Hirose, and Y. Ikehara. 1990. Primary structure of human placental 5′-nucleotidase and identification of the glycolipid anchor in the mature form. Eur. J. Bioochem. 191:563-569.
27. Moxon, E. R., R. A. Deich, and C. Connelly. 1984. Cloning of chromosomal DNA from H. influenzae. Its use for studying the expression of type b capsule and virulence. J. Clin. Investig. 73:298-306.
28. Musher, D. M., K. R. Kubitshek, J. Crennan, and R. E. Baughn. 1983. Pneumonia and acute febrile tracheobronchitis due to Haemophilus influenza. Ann. Intern. Med. 99:344-350.
29. Munson, R. S., Jr., and D. M. Granoff. 1985. Purification and partial characterization of outer membrane proteins P5 and P6 from Haemophilus influenzae type b. Infect. Immun. 49:544-549.
30. Munson, R. S., Jr., and S. Grass. 1988. Purification, cloning, and sequencing of outer membrane protein P1 of Haemophilus influenzae type b. Infect. Immun. 56:2235-2242.
31. Munson, R. S., Jr., and A. Hunt. 1989. Isolation and characterization of a mutant of Haemophilus influenzae type b deficient in outer membrane P1. Infect. Immun. 57:1002-1004.
32. Munson, R. S., Jr., J. L. Shenep, S. J. Barenkamp, and D. M. Granoff. 1983. Purification and comparison of outer membrane protein P2 from Haemophilus influenzae type h isolates. J. Clin. Investig. 72:677-684.
33. Munson, R. S., Jr., and R. W. Tolan, Jr. 1989. Molecular cloning, expression and primary sequence of outer membrane protein P2 of Haemophilus influenzae type b. Infect. Immun. 57:88-94.
34. Murphy, T. F., L. C. Bartos, A. M. Campagnari, M. B. Nelson, K. C. Dudas, and M. A. Apicella. 1986. Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae. Infect. Immun. 54:774-777.
35. Nakai, K., and M. Kanehisa. 1991. Expert system for predicting protein localization sites in Gram-negative bacteria. Proteins Struct. Funct. Genet. 11:95-110.
36. Nelson, M. B., M. A. Apicella, T. F. Murphy, H. Vankeulen, L. D. Spotila, and D. Rekosh. 1988. Cloning and sequencing of Haemophilus influenzae outer membrane protein P6. Infect. Immun. 56:128-134.
37. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10:1-6.
38. Noel, G. J., D. Bauer, and P. J. Edelson. 1992. The role of complement and murine monoclonal antibodies in binding and ingestion of Haemophilus influenzae type b by murine macrophages. J. Infect. Dis. 165(Suppl. 1):S74-S75.
39. Noel, G. J., S. Katz, and P. J. Edelson. 1987. Complement-mediated early clearance of Haemophilus influenzae type b from blood is independent of serum lytic activity. J. Infect. Dis. 157:85-90.
40. Rose, R. E. 1988. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16:355.
41. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
42. Slot, J. W., and H. J. Geuze. 1984. Gold markers for single and double immunolabelling of ultrathin cryosections, p. 129-142. In J. M. Polak, and I. M. Vardell (ed), Immunolabelling for electron microscopy. Elsevier Science Publishing b.v., Amsterdam, The Netherlands.
43. Stenfors, L.-E., and S. Räisänen. 1991. Is attachment of bacteria to the epithelial cells of the nasopharynx the key to otitis media? Int. J. Pediatr. Otorhinolaryngol. 22:1-8.
44. --dependent 5′-nucleotidase of Vibrio parahaemolyticus. J. Biochem. 109:24-29.
45. Toukairin, T., K. Uchino, M. Iwamoo, S. Murakami, T. Tatebayashi, H. Ogawara, and Y. Tonosaki. 1991. New polyphenolic 5′-nucleotidase inhibitors isolated from the wine grape "Koshu" and their biological effects. Chem. Pharm. Bull. 39:1480-1483.
46. van Alphen, L., P. Eijk L. Geelan-van den Broek, and J. Dankert. 1991. Immunochemical characterization of variable epitopes of outer membrane protein P2 of nontypeable Haemophilus influenzae. Infect. Immun. 59:247-252.
47. Walker, R. J., Jr., C. J. Baker, F. J. Quinones, D. G. Hollis, R. E. Weaver, and K. Wiss. 1983. Nontypable Haemophilus influenzae (biotype 4) as a neonatal, maternal, and genital pathogen. Annu. Rev. Infect. Dis. 5:123-135.