Design of a Microfabricated Device for Ligase Detection Reaction (LDR)

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 5345, Issue , 2004, Pages 78-88

  Barrett, Dwhyte O ^a   Maha, Amit ^a   Wang, Yun ^a   Soper, Steven A ^a   Nikitopoulos, Dimitris E ^a   Murphy, Michael C ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

Author keywords

DNA; LDR; LIGA; Micro mixers; PCR; Resistive heaters; Thermoelectric

Indexed keywords

LIGA; LIGASE DETECTION REACTION (LDR); MICRO MIXERS; POLYMERASE CHAIN REACTION (PCR); RESISTIVE HEATERS; THERMOELECTRIC MODULES;

COMPUTER SIMULATION; DNA; ELECTROPHORESIS; FINITE ELEMENT METHOD; GENE TRANSFER; HEATING; MICROPROCESSOR CHIPS; MUTAGENESIS; THERMOELECTRICITY; TUMORS;

ENZYMES;

EID: 2142837118     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.524681     Document Type: Conference Paper

References (24)

1. Mullis, K.B. Ferre, F. F., and Gibbs. R.A. The Polymerase Chain Reaction, Birkhauser, Boston, 1994.
2. Innis, M.A. Myambo, K.B., Gelfand, D.H. and Brow, M.A.D. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA, Proc. Natl. Acad. Sci. USA, vol. 85, no. 24, pp. 9436-9440. 1988.
3. Cheng, J., Shoffner, M.A., Hvichia, G.E., Kricka, L.J., and Wilding, P., Nucleic Acids Research, vol. 24, p. 380, 1996.
4. Woolley, A.T., Hadley, D., Landre, P., DeMello, A.J., Mathies, R.A., and Northrup, M.A., "Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device", Anal. Chem, vol. 68, no. 23, pp. 4081-4086, 1996.
5. Ferrance, J. P., Giordano, B., and Landers, J. P., "Toward Effective PCR-Based Amplification of DNA on Microfabrication Chips", Methods in Molecular Biology, vol. 163, pp. 1-14,2000.
6. Burns, M.A., Johnson, B.N., Brahmasandra, S.N., Handique, K., Websterx, J.R., Krishnan, M., Sammarco, T.S., Man, P.M., Jones, D., Heldsinger, D., Mastrangelo C.H., and Burke, D.T., "An integrated nanoliter DNA analysis device," Science, vol. 282, no. 5388, pp. 484-487, 1998.
7. Burns, M.A., Mastrangelo, C.H., Sammarco, T.S., Man, F.P., Webster, J.R., Johnsons, B.N., Foerster, Jones, B., Fields, D.Y., Kaiser, A.R., and Burke, D.T., "Microfabricated structures for integrated DNA analysis," Proc. Natl. Acad. Sci. USA, vol. 93, no. 11, pp. 5556-5561, 1996.
8. Kopp, M.U., DeMello, A.J., and Manz, A., "Chemical amplification: continuous-flow PCR on a chip", Science, vol. 280, no. 5366, pp. 1046-1048, 1998.
9. Schneegass, R.B., and Kohler, J.M., "Miniaturized flow-through PCR with different template types in a silicon chip thermocycler", Lab on a Chip, vol. 1, no. 1, pp. 42-49, 2001.
10. U.S. Pat # 5,494,810, "Thermostable ligase-mediated DNA ampliications system for the detection of genetic diseases", www.uspto.gov 1996.
11. Barany F., "The ligase chain reaction (LCR) in a PCR world", PCR Methods, Appl.1, 5-16, 1991.
12. Barany F., Day J., Gerry N, Witowski N., Hammer R, Barany G. "Universal DNA Microarray Method for Multiplex Detection of Low Abundance Point Mutations", J. Mol. Biol., 1999, vol. 292, No.2 pp251-262.
13. Mitchell, M. Liu, X., W., Bejat, Y., Ford M.S., Nikitopoulos, D.E., Soper, S.A., Murphy, M. C., "Modeling and Validation of a Molded Polycarbonate Continuous Flow Polymerase Chain Reaction Device", in MicroFluidics, BioMEMS, and Medical Microsystems, ed. H. Becker and P. Wolas, Society of Photo-optical Instrumentation Engineers (SPIE), v 4982,pp. 83-98, 2002.
14. Mitchell M., "Design and Microfabrication of a Molded Polycarbonate Continuous Flow Polymerase Chain Reaction Device", Dept. of Mechanical Engineering, LSU Master's Thesis, 2002.
15. Madou M., Fundamentals of Microfabriction, CRC press, Florida, 1997.
16. Bejat, Y.D., "Micro-Chip Design, Numerical Simulation and Micro-PIV Diagnostics for DNA Assays", Dept. of Mechanical Engineering, Master s Thesis, Louisiana State University, Baton Rouge, LA, 2001.
17. Maha, A, Barrett, D.O., Nikitopoulos, D.E., Soper, S.A., Murphy, M.C., Simulation and Design of Micro-Mixers for Microfluidic Devices, in MicroFluidics, BioMEMS, and Medical Microsystems II, ed. H. Becker and P. Wolas, Society of Photo-optical Instrumentation Engineers (SPIE), 2003.
18. Desta, Y.M., Aigeldinger, G., Zanca, K.J., Coane, P., Gottert, J., Murphy, M.C., "Fabrication of graphite masks for deep and ultra-deep X-ray lithography", in Materials and Devices Characterization in Micromachining III, eds. Y. Vladimirsky, P. J. Coane, Society of Photo-optical Instrumentation Engineers (SPIE), v 4175, pp 122-130, 2000.
19. Qi, S., Ford, S., Liu, X., Barrows, J., Thomas, G., Kelly, K., McCandless, A., Lian, K., Goettert, J., Soper S.A., Hot Embossing High-Aspect-Ratio Microstructures in Poly (Methylmethacrylate) for Constructing Microfluidic Devices with Integrated Components, Lab-on-a-Chip, 2(2): 88-95, 2002.
20. Liu, X., Mitchell. M. W., Bejat, Y., Ford M.S., Nikitopoulos, D.E., Soper, S.A., and Murphy, M. C., "A molded polycarbonate continuous flow polymerase chain reaction device" Submitted to J. MEMS
21. Mills, A.F., Heat Transfer, 2nd ed., Upper Saddle River, NJ: Prentice Hall, Inc., 1999, pp. 277-279.
22. Dryden, H. L., Murnaghan, F. D., and Bateman, H., Hydrodynamics, Bull. No. 84, pp. 197-201.Comm. Hydrodyn, Div. Phy. Sci., Res. Counc., Washington, DC, 1932: reprinted by Dover, New York, 1956.
23. Karnopp, D.C., Rosenberg, R.C., Introduction To Physical System Dynamics, McGraw Hill, Inc., USA, 2000.
24. Beskok A. and Karniadakis G., Micro Flows: Fundamentals and Simulation, Springer-Verlag, New York, 2002.