Structural constraints on the ternary complex of 5-enolpyruvylshikimate-3-phosphate synthase from rotational-echo double-resonance NMR

Journal of Molecular Biology

Volumn 256, Issue 1, 1996, Pages 160-171

  McDowell, Lynda M ^a   Schmidt, Asher ^a,b   Cohen, Eric R ^a,c   Studelska, Daniel R ^a   Schaefer, Jacob ^a  

^a WASHINGTON UNIVERSITY   (United States)

^b TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^c State University of New York Upstate Medical University: College of Medicine   (United States)

Author keywords

5 enolpyruvylshikimate 3 phosphate synthase; Interatomic distances; Protein binding site; Solid state NMR; Stable isotope label

Indexed keywords

3 PHOSPHOSHIKIMATE 1 CARBOXYVINYLTRANSFERASE; ARGININE; GLYPHOSATE; HISTIDINE; LYSINE; PHOSPHATE; PHOSPHOENOLPYRUVATE; PHOSPHONIC ACID DERIVATIVE; SHIKIMIC ACID;

ARTICLE; BINDING SITE; ENZYME STRUCTURE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL;

EID: 0029864204     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0074     Document Type: Article

References (31)

1. Anderson, K. S. & Johnson, K. A. (1990). Kinetic and structural analysis of enzyme intermediates: lessons from EPSP synthase. Chem. Rev. 90, 1131-1149.
2. Anderson, K. S., Sikorski, J. A. & Johnson, K. A. (1988). Evaluation of 5-enolpyruvylshikimate-3-phosphate synthase substrate and inhibitor binding by stopped-flow and equilibrium fluorescence measurements. Biochemistry, 27, 1604-1610.
3. Beusen, D. D., McDowell, L. M., Schmidt, A., Cohen, E. R. & Schaefer, J. (1994). Solid-state NMR determination of the geometry of substrate and inhibitor bound to EPSP synthase. In Peptides: Chemistry, Structure, and Biology (Hodge, R. S. & Smith, J., eds), pp. 760-762, ESCOM, Leiden.
4. 13C NMR of glyphosate: comparison of pH titrations to the herbicidal dead-end complex with 5-enolpyruvylshikimate-3-phosphate synthase. Biochemistry, 28, 3856-3868.
5. 13C]glyphosate bound to enolpyruvylshikimate-3-phosphate synthase. Biochemistry, 32, 2868-2873.
6. Drapeau, G. R., Brammer, W. J. & Yanofsky, C. (1968). Amino acid replacements of the glutamic acid residue at position 48 in the tryptophan synthetase A protein of E. coli. J. Mol. Biol 35, 357-367.
7. Gullion, T. & Schaefer, J. (1989a). Rotational-echo double-resonance NMR. J. Magn. Reson. 81, 196-200.
8. Gullion, T. & Schaefer, J. (1989b). Detection of weak heteronuclear dipolar coupling by rotational-echo double-resonance NMR. Advan. Magn. Reson. 13, 57-83.
9. Gullion, T. & Schaefer, J. (1991). Elimination of resonance offset effects in rotational-echo, double-resonance NMR. J. Magn. Reson. 92, 439-442.
10. Gullion, T. G., Baker, D. B. & Conradi, M. S. (1990). New, compensated Carr-Purcell sequences. J. Magn. Reson. 89, 479-484.
11. Hing, A. W., Vega, S. & Schaefer, J. (1993). Measurement of heteronuclear dipolar coupling by transferred-echo double-resonance NMR. J. Magn. Reson. 103, 151-162.
12. Hing, A. W., Tjandra, N., Cottam, P. F., Schaefer, J. & Ho, C. (1994). An investigation of the ligand-binding site of the glutamine-binding protein of E. coli using rotational-echo double-resonance NMR. Biochemistry, 33, 8651-8661.
13. Holl, S. M., McKay, R. A., Gullion, T. & Schaefer, J. (1990). Rotational-echo triple-resonance NMR. J. Magn. Reson. 89, 620-626.
14. Holl, S. M., Marshall, G. R., Beusen, D. D., Kociolek, K., Redlinski, A. S., Leplawy, M. T., McKay, R. A., Vega, S. & Schaefer, J. (1992). Determination of an 8-Å interatomic distance in a helical peptide by solid-state NMR. J. Am. Chem. Soc. 114, 4830-4833.
15. 15N NMR studies of alanine metabolism in Aerococcus viridans. J. Biol. Chem. 260, 2777-2781.
16. Marshall, G. R., Beusen, D. D., Kociolek, K., Redlinski, A. S., Leplawy, M. T., Pan, Y. & Schaefer, J. (1990). Determination of a precise interatomic distance in a helical peptide by REDOR NMR. J. Am. Chem. Soc. 112, 963-966.
17. McDowell, L. M., Schmidt, A., Christenson, A. M., Beusen, D. D. & Schaefer, J. (1993). Rotational-echo double-resonance NMR defines the relative geometry of substrate and inhibitor bound to 5-enolpyruvylshikimate-3-phosphate synthase. In 34th Experimental NMR Conference, St. Louis, MO.
18. McDowell, L. M., Schmidt, A., Cohen, E. R., Beusen, D. D. & Schaefer, J. (1994). Characterizing the EPSP synthase binding site: the TEDOR-REDOR combination. In 35th Experimental NMR Conference, Asilomar, CA.
19. McDowell, L. M., Klug, C. A., Beusen, D. D. & Schaefer, J. (1995). Ligand geometry of the ternary complex of 5-enolpyruvylshikimate-3-phosphate synthase from rotational double-resonance NMR. Biochemistry (in review).
20. McKay, R. A. (1993). Efficiency of transmission-line probes. In 34th Experimental NMR Conference, St. Louis, MO.
21. Morris, G. A. & Freeman, R. (1979). Enhancement of NMR signals by polarization transfer. J. Am. Chem. Soc. 101, 760.
22. Mueller, D. D., Schmidt, A., Pappan, K. L., McKay, R. A. & Schaefer, J. (1995). Activator carbamino carbon to inhibitor phosphorus internuclear distances in ribulose 1,5-bisphosphate carboxylase/oxygenase. A solid state NMR study Biochemistry, 34, 5597-5603.
23. Olins, P. O., Devine, C. S., Rangwala, S. H. & Kavka, K. S. (1988). The T7 phage gene 1φ leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in E. coli. Gene, 73, 227-235.
24. Schaefer, J. (1995). REDOR NMR of stable-isotope-labeled protein binding sites. In Stable Isotope Applications in Biomolecular Structure and Mechanisms, pp. 187-207, document LA-12893-C, National Technical Information Service, US Department of Commerce.
25. Schaefer, J., Garbow, J. R., Stejskal, E. O. & Lefelar, J. A. (1987). Plasticization of poly(butyral-co-vinyl alcohol). Macromolecules, 20, 1271-1278.
26. Schmidt, A., Cohen, E. R., McDowell, L. M., Beusen, D. D. & Schaefer, J. (1993). Structural study of the binding site of EPSP synthase with bound substrate (S3P) and inhibitor (glyphosate) by REDOR and TEDOR. In 34th Experimental NMR Conference, St. Louis, MO.
27. Stallings, W. C., Abdel-Meguid, S. S., Lim, L. W., Shief, H. S., Dayringer, H. E., Leimgruber, N. K., Stegman, R. A., Anderson, K. S., Sikorski, J. A., Padgette, S. R. & Kishore, G. M. (1991). Structure and topological symmetry of the glyphosate target 5-enolpyruvylshikimate-3-phosphate synthase: a distinctive protein fold. Proc. Natl Acad. Sci. USA, 88, 5046-5050.
28. Steinrucken, H. C. & Amrhein, N. (1984a). 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumosniae. 1. Purification and properties. Eur. J. Biochem. 143, 341-349.
29. Steinrucken, H. C. & Amrhein, N. (1984b). 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 2. Inhibition by glyphosate (N-[phosphonomethyl]glycine). Eur. J. Biochem. 143, 351-357.
30. 13C NMR in solids. Faraday Symp. 13, 56-62.
31. 19F NMR. J. Am. Chem. Soc. (in review).