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23
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21344452884
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note
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6.
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0141753951
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The calix[4]arene carboxylic acid 1 was synthesized according to the literature methods: (a) A. Arduini, A. Pochini, S. Reverberi and R. Ungaro, J. Chem. Soc., Chem. Commun., 1984, 981;
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Arduini, A.1
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Reverberi, S.3
Ungaro, R.4
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21344442027
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note
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1 mg of the TOPO capped CdSe-ZnS QDs was dispersed in 1 mL of tetrahydrofuran and 30 mg of calix[4]arene carboxylic acids 1 was added. After the mixed solution was sonicated for 30 s using a bath type sonicator, 3 mL of dimethylformamide was added. Then, 30 mg of potassium tert-butoxide was slowly added for the deprotonation of the carboxylic groups of the calix[4]arene. The resulting precipitate consisting of the QDs coated by 1 was separated using a centrifuge. The sedimented precipitate was then dispersed in 20 mL water. The aqueous QD dispersion was sonicated for 5 min and filtered using a 0.2 μm disposal filter.
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21344451961
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note
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The quantum yield of the 1-coated QDs with an emission peak at 535 nm is not estimated because the absorption of the QDs does not show a distinct peak.
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28
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21344453384
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note
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2, where I(τ)is the fluorescence intensity at the time τ, and δI(τ) = I(τ) - 〈I(τ)〉. The brackets denote ensemble average.
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0042660351
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21344449408
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note
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i are the radius and diffusion time of the fluorophore i.
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34
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21344468943
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note
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For the 1-coated QDs with emission peaks at 535 nm and 575 nm, the hydrodynamic size was determined to be 6.2 nm and 7.9 nm in diameter, respectively.
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35
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0029847806
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The GFP molecule forms a nearly perfect cylinder 4.2 nm long and 2.4 nm in diameter. See: M. Ormö, A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien and S. J. Remington, Science, 1996, 273, 1392.
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Tsien, R.Y.5
Remington, S.J.6
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36
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0003648954
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Academic Press, New York
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-1) was added. Then 10 μL of GFP antibody (5 μM) was added to the QDs aqueous solution and coupling reaction was allowed to proceed at room temperature. From the measurements of fluorescence autocorrelation curves of the QDs, it was found that the reaction was completed within 10 min. For the coupling reaction using EDC, see: G. T. Hermanson, Bioconjugate Techniques, Academic Press, New York, 1996.
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(1996)
Bioconjugate Techniques
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Hermanson, G.T.1
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Calixarene 2001
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Asfari, Z.1
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