Improved protein identification through the use of unstained gels

Journal of Proteome Research

Volumn 4, Issue 3, 2005, Pages 992-997

  Loiselle, David R ^a   Thelin, William R ^b   Parker, Carol E ^a   Dicheva, Nedyalka N ^a   Kesner, Barry A ^b   Mocanu, Viorel ^a   Wang, Frank ^c   Milgram, Sharon L ^b   Warren, Maria R Esteban ^a   Borchers, Christoph H ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c BioMachines Inc   (United States)

Author keywords

Automation; Immunoprecipitation; MALDI MS; Page; Protein identification; Robotics; Silver staining; Unstained gels

Indexed keywords

APROTININ; BETA GALACTOSIDASE; BOVINE SERUM ALBUMIN; CARBONATE DEHYDRATASE; GLYCOGEN PHOSPHORYLASE B; LYSOZYME; MYOSIN; OVALBUMIN; TRYPSIN INHIBITOR;

ARTICLE; IMMUNOPRECIPITATION; INTERMETHOD COMPARISON; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; ROBOTICS; SILVER STAINING;

ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GELS; HUMANS; PROTEINS; ROBOTICS; SILVER STAINING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 20844439527     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr049785o     Document Type: Article

References (17)

1. Lopez, M. F.; Berggren, K.; Chernokalskaya, E.; Lazarev, A.; Robinson, M.; Patton, W. F. A comparison of silver stain and Sypro ruby protein gel stain with respect to protein detection in two-dimensional gels and identification by peptide mass profiling. Electrophoresis 2000, 21, 3673-3683.
2. Malone, J. P.; Radabaugh, M. R.; Leimgruber, R. M.; Gerstenecker, G. S. Practical aspects of fluorescent staining for proteomic applications. Electrophoresis 2001, 22, 919-932.
3. Lanne, B.; Panfilov, O. Protein staining influences the quality of mass spectra obtained by peptide mass fingerprinting after separation on 2-D gels. A comparison of staining with coomassie brilliant blue and Sypro ruby. J. Proteome Res. 2005, 4, 175-179.
4. Patton, W. F. Detection technologies in proteome analysis. J. Chromatogr. B 2002, 771, 3-31.
5. Merrill, C.; Goldman, D.; Van Keuren, M. Methods Enzymol. 1984, 104, 441-447.
6. Syrovy, I.; Hodny, Z. Staining and quantification of proteins separated by polyacrylamide gel electrophoresis. J. Chromatogr. 1991, 569, 175-196.
7. Scheler, C.; Lamer, S.; Pan, Z.; Li, X. P.; Salnikow, J.; Jungblut, P. Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Electrophoresis 1998, 19, 918-927.
8. Shevchenko, A.; Wilm, M.; Vorm, O.; Mann, M. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 1996, 68, 850-858.
9. Harry, J. L.; Wilkins, M. R.; Herbert, B. R.; Packer, N. H.; Gooley, A. A.; Williams, K. L. Proteomics: Capacity versus utility. Electrophoresis 2000, 21, 1071-1081.
10. Sluszny, C.; Yeung, E. S. One- and two-dimensional miniaturized electrophoresis of proteins with native fluorescence detection. Anal. Chem. 2004, 76, 1359-1365.
11. Roegener, J.; Lutter, P.; Reinhardt, R.; Blueggel, M.; Meyer, H. E.; Anselmetti, D. Ultrasensitive detection of unstained proteins in acrylamide gels by 2003native UV fluorescence. Anal. Chem. 2003, 75, 157-159.
12. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage t4. Nature 1970, 227, 680-685.
13. Parker, C. E.; Warren, M. R.; Loiselle, D. R.; Dicheva, N. N.; Horchers, C. H. "Identification of components of protein complexes" In Methods in Molecular Biology, Patterson, W. C., Ed.; Humana Press: Patterson, NJ, 2004; Vol. in press.
14. Sarkadi, B.; Price, E. M.; Boucher, R. C.; Germann, U. A.; Scarborough, G. A. Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane atpase. J. Biol. Chem. 1992, 267, 4854-4858.
15. Borchers, C.; Peter, J. F.; Hall, M. C.; Kunkel, T. A.; Tomer, K. B. Identification of in-gel digested proteins by complementary peptide-mass fingerprinting and tandem mass spectrometry data obtained on an electrospray ionization quadrupole time-of-flight mass spectrometer. Anal. Chem. 2000, 72, 1163-1168.
16. www.matrixscience.com.
17. Mohler, P. J.; Kreda, S. M.; Boucher, R. C.; Sudol, M.; Stutts, M. J.; Milgram, S. L. Yes-associated protein 65 localizes p62(c-yes) to the apical compartment of airway epithelia by association with ebp50. J. Cell Biol. 1999, 147, 879-890.