Protein staining influences the quality of mass spectra obtained by peptide mass fingerprinting after separation on 2-D gels. A comparison of staining with Coomassie Brilliant Blue and Sypro Ruby

Journal of Proteome Research

Volumn 4, Issue 1, 2005, Pages 175-179

  Lanne, Boel ^a   Panfilov, Oleg ^a  

^a ASTRAZENECA R AND D   (Sweden)

Author keywords

Cystein residues; Gel electrophoresis; MALDI; Mass spectrometry; Peptide mass fingerprinting; Protein identification; Protein staining; Proteomics; Significance testing

Indexed keywords

BRILLIANT BLUE; COOMASSIE BRILLIANT BLUE; DYE; FLUORESCENT DYE; PEPTIDE; PROTEIN; SYPRO RUBY; UNCLASSIFIED DRUG;

ARTICLE; COMPARATIVE STUDY; GEL; MASS SPECTROMETRY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUALITATIVE ANALYSIS; SEPARATION TECHNIQUE; SIGNAL NOISE RATIO; STAINING;

ANIMALS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; EUROPIUM; HUMANS; MASS SPECTROMETRY; PROTEINS; ROSANILINE DYES; STAINING AND LABELING;

EID: 14844322255     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr040005l     Document Type: Article

References (6)

1. Berggren, K.; Chernokalskaya, E.; Steinberg, T. H.; Kemper, C.; Lopez, M. F.; Diwu, Z.; Haugland, R. P.; Patton, W. F. Electrophoresis 2000, 21, 2509-2521.
2. Berggren, K. N.; Schulenberg, B.; Lopez, M. F.; Steinberg, T. H.; Bogdanova, A.; Smejkal, G.; Wang, A.; Patton, W. F. Proteomics 2002, 2, 486-498.
3. Voshols, J.; Novartis Pharma, F. G. A. P. S. U., Basel, Switzerland 2003, Personal communication.
4. Lanne, B.; Potthast, F.; Höglund, Å.; Brockenhuus von Lowenhielm, H.; Nyström, A.-C.; Nilsson, F.; Dahllöf, B. Proteomics 2001, 1, 819-828.
5. Görg, A.; Postel, W.; Weser, J.; Günter, S.; Strahler, J. R.; Hanash, S. M.; Somerlot, L. Electrophoresis 1987, 8, 122-124.
6. Doherty, N.; Littman, B.; Reilly, K.; Swindell, A.; Buss, J.; Anderson, N. L. Electrophoresis 1998, 19, 355-363.