FL-CTL assay: Fluorolysometric determination of cell-mediated cytotoxicity using green fluorescent protein and red fluorescent protein expressing target cells

Journal of Immunological Methods

Volumn 300, Issue 1-2, 2005, Pages 100-114

  Chen, Kong ^a   Chen, Liansheng ^a   Zhao, Peilin ^a   Marrero, Luis ^a   Keoshkerian, Elizabeth ^a   Ramsay, Alistair ^a   Cui, Yan ^a  

^a LOUISIANA STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antigen specific CTL function; CTL assay; Cytotoxic T lymphocytes; Fluorescent proteins; Fluorometric based CTL; GFP

Indexed keywords

GREEN FLUORESCENT PROTEIN; ISOLEUCYLTYROSYLSERYLTHREONYLVALYLALANYLSERYLSERYLLEUCINE; LENTIVIRUS VECTOR; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; PEPTIDE DERIVATIVE; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ACCURACY; ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN PRESENTATION; ARTICLE; CELL LINE; CELL MEDIATED CYTOTOXICITY; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; ECONOMIC ASPECT; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROPLATE READER; FLUORESCENCE MICROSCOPY; INTERMETHOD COMPARISON; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RELIABILITY; REPRODUCIBILITY; SCALE UP; SENSITIVITY ANALYSIS; T LYMPHOCYTE;

ANIMALS; CELL LINE, TUMOR; CYTOTOXICITY TESTS, IMMUNOLOGIC; FLOW CYTOMETRY; FLUOROMETRY; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LENTIVIRUS; LUMINESCENT PROTEINS; MICE; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES, CYTOTOXIC;

EID: 20444390118     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2005.02.010     Document Type: Article

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