Mutagenesis at Asp27 of pyruvate decarboxylase from Zymomonas mobilis. Effect on its ability to form acetoin and acetolactate

European Journal of Biochemistry

Volumn 267, Issue 21, 2000, Pages 6493-6500

  Wu, Yong Ge ^a   Chang, Alan K ^b   Nixon, Peter F ^b   Li, Wei ^a   Duggleby, Ronald G ^b  

^a JILIN UNIVERSITY   (China)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Acetoin; Acetolactate; Pyruvate decarboxylase; Site directed mutagenesis; Thiamin diphosphate

Indexed keywords

ACETALDEHYDE; ACETOIN; ALANINE; ASPARTIC ACID; COCARBOXYLASE; DIVALENT CATION; MAGNESIUM ION; PYRUVATE DECARBOXYLASE; PYRUVIC ACID;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME STRUCTURE; MICHAELIS CONSTANT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; THERMODYNAMICS; ZYMOMONAS MOBILIS;

ACETALDEHYDE; ACETOIN; AMINO ACID SUBSTITUTION; ASPARTIC ACID; BINDING SITES; CATALYSIS; KINETICS; LACTATES; MODELS, CHEMICAL; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; PYRUVATE DECARBOXYLASE; PYRUVIC ACID; RECOMBINANT PROTEINS; ZYMOMONAS;

EID: 0033756087     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01744.x     Document Type: Article

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