Identification of key amino acids responsible for the substantially higher affinities of human type 1 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD1) for substrates, coenzymes, and inhibitors relative to human 3β-HSD2

Journal of Biological Chemistry

Volumn 280, Issue 22, 2005, Pages 21321-21328

  Thomas, James L ^a   Boswell, Elizabeth L ^a   Scaccia, Launa A ^a   Pletnev, Vladimir ^b,c   Umland, Timothy C ^b,d  

^a MERCER UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b HAUPTMAN WOODWARD MEDICAL RESEARCH INSTITUTE   (United States)

^c SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^d UNIVERSITY AT BUFFALO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMERS; DISEASE CONTROL; ENZYME INHIBITION; ENZYME KINETICS; GENETIC ENGINEERING; HUMAN ENGINEERING; REACTION KINETICS; SUBSTRATES;

ENZYME SUBUNIT; HOMODIMER; ISOFORMS; KINETIC PROFILES;

AMINO ACIDS;

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; 3BETA HYDROXYSTEROID DEHYDROGENASE 1; 3BETA HYDROXYSTEROID DEHYDROGENASE 2; ARGININE; DIMER; EPOSTANE; GENE PRODUCT; GLUTAMINE; HISTIDINE; ISOENZYME; ISOMERASE; MUTANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; STEROID; TRILOSTANE; TYROSINE; UNCLASSIFIED DRUG;

ALLOSTERISM; AMINO ACID ANALYSIS; ARTICLE; BINDING AFFINITY; CANCER HORMONE THERAPY; COENZYME; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME SUBSTRATE COMPLEX; ENZYME SUBUNIT; MOLECULAR INTERACTION; MOLECULAR MODEL; MUTATION; PREMATURE LABOR; PRIORITY JOURNAL; PROTEIN EXPRESSION; TISSUE SPECIFICITY; WILD TYPE;

3-HYDROXYSTEROID DEHYDROGENASES; ALLOSTERIC SITE; AMINO ACID SEQUENCE; ANDROSTENOLS; ANIMALS; ARGININE; BACULOVIRIDAE; BLOTTING, WESTERN; CATALYSIS; CATALYTIC DOMAIN; CELL LINE; DIHYDROTESTOSTERONE; DNA PRIMERS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUTAMINE; HISTIDINE; HUMANS; INSECTS; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; NAD; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TISSUE DISTRIBUTION;

EID: 20444366185     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M501269200     Document Type: Article

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