Molecular cytogenetic analysis of recently evolved Tragopogon (Asteraceae) allopolyploids reveal a karyotype that is additive of the diploid progenitors

American Journal of Botany

Volumn 91, Issue 7, 2004, Pages 1022-1035

  Pires, J Chris ^a,f   Lim, K Yoong ^b   Kovarík, Ales ^c   Matyásek, Roman ^c   Boyd, Amy ^a   Leitch, Andrew R ^b   Leitch, Ilia J ^a   Bennett, Michael D ^a   Soltis, Pamela S ^d   Soltis, Douglas E ^e  

^a ROYAL BOTANIC GARDENS   (United Kingdom)

^b QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^c INSTITUTE OF BIOPHYSICS   (Czech Republic)

^d UNIVERSITY OF FLORIDA   (United States)

^e UNIVERSITY OF FLORIDA   (United States)

^f UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Centromere; Chromosomal evolution; Fluorescent in situ hybridization (FISH); Genome size; Polyploidy; rDNA; Subtelomere; Tragopogon

Indexed keywords

CHROMOSOME; CYTOGENETICS; EVOLUTION; GENOME; POLYPLOIDY;

ASTERACEAE; TRAGOPOGON; TRAGOPOGON DUBIUS; TRAGOPOGON MIRUS; TRAGOPOGON MISCELLUS; TRAGOPOGON PORRIFOLIUS; TRAGOPOGON PRATENSIS;

EID: 20144374329     PISSN: 00029122     EISSN: 00029122     Source Type: Journal    
DOI: 10.3732/ajb.91.7.1022     Document Type: Article

References (124)

1. ABBOTT, R. J., AND A. J. LOWE. In press. Origins, establishment and evolution of two new polyploid species of Senecio in the British Isles. Biological Journal of the Linnean Society.
2. ADAMS, K. L., AND J. F. WENDEL. In press. Exploring the genomic mysteries of polyploidy in cotton. Biological Journal of the Linnean Society.
3. AINOUCHE, M. L., A. BAUMEL, AND A. SALMON. In press. Spartina anglica Schreb.: a natural model system for analysing early evolutionary changes that affect allopolyploid genomes. Biological Journal of the Linnean Society.
4. ASHTON, P. A., AND R. J. ABBOTT. 1992. Multiple origins and genetic diversity in the newly arisen allopolyploid species, Senecio cambrensis Rosser (Compositae). Heredity 68: 25-32.
5. BABCOCK, E. B., AND G. L. STEBBINS. 1938. The American species of Crepis: their relationships and distribution as affected by polyploidy and apomixis. Carnegie Institute of Washington, Publication No. 504.
6. BALDWIN, B. G. 1993. Molecular phylogenetics of Calycadenia (Compositae) based on ITS sequencing of nuclear ribosomal DNA - chromosomal and morphological evolution reeaximined. American Journal of Botany 80: 222-238.
7. BAUMEL, A., M. L. AINOUCHE, R. KALENDAR, AND A. H. SCHULMAN. 2002. Retrotransposons and genomic stability in populations of the young allopolyploid species Spartina anglica C. E. Hubbard (Poaceae). Molecular Biology and Evolution 19: 1218-1227.
8. BAUMEL, A., M. L. AINOUCHE, AND J. E. LEVASSEUR. 2001. Molecular investigations in populations of Spartina anglica C. E. Hubbard (Poaceae) invading coastal Brittany (France). Molecular Ecology 10: 1689-1701.
9. BROWN, R. K., AND C. G. SCHAAK. 1972. Two new species of Tragopogon for Arizona. Madroño 21: 304.
10. CARR, G. 1985. Monograph of the Hawaiian Madiinae (Asteraceae). Allertonia 4: 1-123.
11. CERBAH, M., J. COULAUD, S. C. BROWN, AND S. SILJAK-YAKOVLEV. 1999. Evolutionary DNA variation in the genus Hypochaeris. Heredity 82: 261-266.
12. CHEN, Z. J., ET AL. In press. The development of an Arabidopsis model system for genome-wide analysis of polyploidy effects. Biological Journal of the Linnean Society.
13. CLAUSEN, J., D. D. KECK, AND W. M. HIESEY. 1945. Experimental studies on the nature of species. II. Plant evolution through amphiploidy and autoploidy, with examples from the Madiinae. Carnegie Institute of Washington, Publication No. 564.
14. COOK, L. M., P. S. SOLTIS, S. J. BRUNSFELD, AND D. E. SOLTIS. 1998. Multiple independent formations of Tragopogon tetraploids (Asteraceae): evidence from RAPD markers. Molecular Ecology 7: 1293-1302.
15. COX, A. V., S. T. BENNETT, A. S. PARAKONNY, A. KENTON, M. A. CALLIMASSIA, AND M. D. BENNETT. 1993. Comparison of plant telomere locations using a PCR-generated synthetic probe. Annals of Botany 72: 239-247.
16. CROWHURST, R. N., AND R. C. GARNDER. 1991. A genome-specific repeat sequence from kiwifruit (Actinidia deliciosa var. deliciosa). Theoretical and Applied Genetics 81: 71-78.
17. CULLIS, C. A. 1979. Quantitative variation of ribosomal RNA genes in flax genotrophs. Heredity 42: 237-246.
18. DOYLE, J. J., AND J. L. DOYLE. 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
19. ESPINÁS, M. L., AND M. CARBALLO. 1993. Pulsed-field gel-electrophoresis analysis of higher-order chromatin structures of Zea mays - highly methylated DNA in the 50 kb chromatin structure. Plant Molecular Biology 21: 847-857.
20. FITZERALD, D. J., G. L. DRYDEN, E. C. BRONSON, J. S. WILLIAMS, AND J. N. ANDERSON. 1994. Conserved patterns of bending satellite and nucleosome positioning DNA. Journal of Biological Chemistry 269: 21 303-21 314.
21. FRANCKI, M. G. 2001. Identification of Bilby, a diverged centromeric Ty1-copia retrotransposon family from cereal rye (Secale cereale L.). Genome 44: 266-274.
22. FULNECEK, J., R. MATYÁSEK, AND A. KOVARÍK. 2002. Distribution of 5-methylcytosine residues in 5S rRNA genes in Arabidopsis thaliana and Secale cereale. Molecular Genetics and Genomics 268: 510-517.
23. GANAL, M. W., N. L. V. LAPITAN, AND S. D. TANKSLEY. 1991. Macrostructures of tomato telomeres. Plant Cell 3: 87-94.
24. GERLACH, W. L., AND J. R. BEDBROOK. 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Research 7: 1869-1885.
25. GOODSELL, D. S., M. KACZORGRZESKOWIAK, AND R. E. DICKERSON. 1994. The crystal structure of C-C-A-T-T-A-A-T-G-G- implications for bending of B-DNA at T-A steps. Journal of Molecular Biology 239: 79-96.
26. GRANT, V. 1981. Plant speciation. Columbia University Press, New York, New York, USA.
27. GREILHUBER, J. 1998. Intraspecific variation in genome size: a critical reassessment. Annals of Botany 82(Supplement A): 27-35.
28. GUERRA, M. 2000. Patterns of heterochromatin distribution in plant chromosomes. Genetics and Molecular Biology 23: 1029-1041.
29. GUSTAFSSON, A. 1932. Zytologische und experimentelle Studien in der Gattung Taraxacum. Hereditas 16: 41-62.
30. HANSON, L., K. A. MCMAHON, M. A. T. JOHNSON, AND M. D. BENNETT. 2001. First nuclear DNA C-values for 25 angiosperm families. Annals of Botany 87: 251-258.
31. HANSON, R. E., X-P. ZHAO, M. N. ISLAM-FARIDI, A. H. PATERSON, M. S. ZWICK, C. F. CRANE, T. D. MCKNIGHT, D. M. STELLY, AND H. J. PRICE. 1998. Evolution of interspersed repetitive elements in Gossypium (Malvaceae). American Journal of Botany 85: 1364-1368.
32. HENNIG, W. 1999. Heterochromatin. Chromosoma 108: 1-9.
33. HESLOP-HARRISON, J. S. 1991. The molecular cytogenetics of plants. Journal of Cell Science 100: 15-21.
34. HESLOP-HARRISON, J. S. 2000. Comparative genome organization in plants: from sequence and markers to chromatin and chromosomes. Plant Cell 12: 617-635.
35. HESLOP-HARRISON, J. S., AND M. D. BENNETT. 1990. Nuclear architecture in plants. Trends in Genetics 6: 401-405.
36. HESLOP-HARRISON, J. S., M. MURATA, Y. OGURA, T. SCHWARZACHER, AND F. MOTOYOSHI. 1999. Polymorphisms and genome organization of repetitive DNA from centromeric regions of Arabidopsis chromosomes. Plant Cell 11: 31-42.
37. HOUBEN, A., G. WANNER, L. HANSON, D. VERLIN, C. R. LEACH, AND J. N. TIMMIS. 2000. Cloning and characterisation of polymorphic heterochromatic segments of Brachycome dichromosomatica. Chromosoma 109: 206-213.
38. JAMILENA, M., M. GARRIDO-RAMOS, C. RUIZ REJON, M. RUIZ REJON, AND J. S. PARKER. 1995. Characterisation of repeated sequences from microdissected B chromosomes of Crepis capillaris. Chromosoma 104: 113-120.
39. JAMILENA, M., C. RUIZ REJON, AND M. RUIZ REJON. 1993. Repetitive DNA sequence families in Crepis capillaris. Chromosoma 102: 272-278.
40. JIANG, J., AND B. S. GILL. 1994. Non isotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37: 717-725.
41. JIANG, J., AND B. S. GILL. 1996. Current status and potential of fluorescence in situ hybridization in plant genome mapping. In A. H. Paterson [ed.], Genome mapping in plants, 127-135. R. G. Landes Co., Austin, Texas, USA.
42. KENTON, A., A. S. PAROKONNY, Y. Y. GLEBA, AND M. D. BENNETT. 1993. Characterization of the Nicotiana-Tabacum-L genome by molecular cytogenetics. Molecular & General Genetics 240: 159-169.
43. KISHII, M., K. NAGAKI, T. HISASHI, AND T. SASAKUMA. 1999. Exclusive localization of tandem repetitive sequences in subtelomeric regions of Leymus racemosus (Poaceae, Triticeae). Chromosome Research 7: 519-529.
44. KISHII, M., K. NAGAKI, AND H. TSUJIMOTO. 2001. A tandem repetitive sequence located in the centromeric region of common wheat (Triticum aestivum) chromosomes. Chromosome Research 9: 417-428.
45. KOTSERUBA, V., D. GERNAND, A. MEISTER, AND A. HOUBEN. 2003. Uniparental loss of ribosomal DNA in the allotetraploid grass Zingeria trichopoda (2n = 8). Genome 46: 156-163.
46. KOVARÍK, A., B. KOUKALOVA, K. Y. LIM, R. MATYÁSEK, C. P. LICHTENSTEIN, A. R. LEITCH, AND M. BEZDEK. 2000. Comparative analysis of DNA methylation in tobacco heterochromatic sequences. Chromosome Research 8: 527-541.
47. KOVARÍK, A., R. MATYÁSEK, K. Y. LIM, K. SKALICKA, B. KOUKALOVA, S. KNAPP, M. W. CHASE, AND A. R. LEITCH. In press. Concerted evolution of 18-5.8-6S rDNA repeats in Nicotiana allotetraploids. Biological Journal of the Linnean Society.
48. LANGDON, T., C. SEAGO, R. N. JONES, H. OUGHAM, H. THOMAS, J. W. FORSTER, AND G. JENKINS. 2000. De novo evolution of satellite DNA on the rye B chromosome. Genetics 154: 869-884.
49. LAWRENCE, W. J. C. 1929. The genetics and cytology of Dahlia species. Journal of Genetics 21: 125-159.
50. LEITCH, A. R., T. SCHWARZACHER, D. JACKSON, AND I. J. LEITCH. 1994. In situ hybridization: a practical guide. Bios, Oxford, UK.
51. LEITCH, I. J., AND M. D. BENNETT. 1997. Polyploidy in angiosperms. Trends in Plant Science 2: 470-476.
52. LEITCH, I. J., AND M. D. BENNETT. In press. Genome downsizing in polyploids. Biological Journal of the Linnean Society.
53. LEVIN, D. A. 1983. Polyploidy and novelty in flowering plants. American Naturalist 122: 1-25.
54. LEVIN, D. A. 2002. The role of chromosomal change in plant evolution. Oxford University Press, New York, New York, USA.
55. LEVY, A. A., AND M. FELDMAN. 2002. The impact of polyploidy on grass genome evolution. Plant Physiology 130: 1587-1593.
56. LEVY, A. A., AND M. FELDMAN. In press. Genetic and epigenetic reprogramming of the wheat genome upon allopolyploidization. Biological Journal of the Linnean Society.
57. LIM, K. Y., A. KOVARÍK, R. MATYÁSEK, M. BEZDEK, C. P. LICHTENSTEIN, AND A. R. LEITCH. 2000a. Gene conversion of ribosomal DNA in Nicotiana tabacum is associated with undermethylated, decondensed and probably active gene units. Chromosoma 109: 161-172.
58. LIM, K. Y., R. MATYÁSEK, A. KOVARÍK, J. FULNECEK, AND A. R. LEITCH. 2004. Genome evolution in allotetraploid Nicotiana. Biological Journal of the Linnean Society, in press.
59. LIM, K. Y., R. MATYÁSEK, C. P. LICHTENSTEIN, AND A. R. LEITCH. 2000b. Molecular cytogenetic analyses and phylogenetic studies in the Nicotiana section Tomentosae. Chromosoma 109: 245-258.
60. LIU, B., C. L. BRUBAKER, R. C. CRONN, AND J. F. WENDEL. 2001. Polyploid formation in cotton is not accompanied by rapid genomic changes. Genome 43: 874-880.
61. LIU, B., AND J. F. WENDEL. 2002. Non-Mendelian phenomena in allopolyploid genome evolution. Current Genomics 3: 489-506.
62. MACAS, J., T. MESZAROS, AND M. NOUZOVA. 2002. PlantSat: a specialized database for plant satellite repeats. Bioinformatics 18: 28-35.
63. MADLUNG, A. R., R. W. MASUELLI, B. WATSON, S. H. REYNOLDS, J. DAVIDSON, AND L. COMAI. 2002. Remodeling of DNA methylation and phenotypic and transcriptional changes in synthetic Arabidopsis allotetraploids. Plant Physiology 129: 733-746.
64. MALUSZYNSKA, J., AND J. S. HESLOP-HARRISON. 1991. Localization of tandemly repeated DNA sequences in Arabidopsis thaliana. Plant Journal 1: 159-166.
65. MARCHI, P., O. ILLUMINATI, A. MACIOCE, R. CAPINERI, AND G. DAMATO. 1983. Genome evolution and polyploidy in Leucanthemum vulgare Lam. Aggr (Compositae) - karyotype analysis and DNA microdensitometry. Caryologia 36: 1-18.
66. MARTINEZ-ZAPATER, J. M., M. A. ESTELLE, AND C. R. SOMERVILLE. 1986. A highly repeated DNA sequences in Arabidopsis thaliana. Molecular & General Genetics 204: 417-423.
67. MASTERSON, J. 1994. Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science 264: 421-424.
68. MATZKE, M. A., O. MITTELSTEN SCHEID, AND A. J. M. MATZKE. 1999. Rapid structural and epigenetic changes in polyploid and aneuploid genomes. Bioessays 21: 761-767.
69. MESTROVIC, N., B. MRAVINEC, C. JUAN, D. UGARKOVIC, AND M. PLOHL. 2000. Comparative study of satellite sequences and phylogeny of five species from the genus Palorus (Insecta, Coleoptera). Genome 43: 776-785.
70. MEYER, P., I. NIEDENHOF, AND M. TEN LOHUIS. 1994. Evidence for cytosine methylation of nonsymmetrical sequences in transgenic Petunia hybrida. EMBO Journal 13: 2084-2088.
71. MILLER, J. T., F. DONG, S. A. JACKSON, J. SONG, AND J. JIANG. 1998. Retrotransposon-related DNA sequences in the centromeres of grass chromosomes. Genetics 150: 1615-1623.
72. MISHIMA, M., N. OHMIDO, K. FUKUI, AND T. YAHARA. 2002. Trends in site-number change of rDNA loci during polyploid evolution in Sanguisorba (Rosaceae). Chromosoma 110: 550-558.
73. NAGAKI, K., H. TSUJIMOTO, AND T. SASAKUMA. 1999. A novel repetitive sequence, termed the JNK repeat family, located on an extra heterochromatic region of chromosome 2R of Japanese rye. Chromosome Research 6: 95-101.
74. NARYAN, R. K. J. 1998. The role of genomic constraints upon evolutionary changes in genome size and chromosome organization. Annals of Botany 82(Supplement A): 57-66.
75. NAVASHIN, M. 1934. Chromosomal alterations caused by hybridization and their bearing upon certain genetic problems. Cytologia 5: 169-203.
76. OSBORN, T. C., J. C. PIRES, J. A. BIRCHLER, D. L. AUGER, Z. J. CHEN, H.-S. LEE, L. COMAI, A. MADLUNG, R. W. DOERGE, V. COLOT, AND R. A. MARTIENSSEN. 2003. Understanding mechanisms of novel gene expression in polyploids. Trends in Genetics 19: 141-147.
77. OWNBEY, M. 1950. Natural hybridization and amphiploidy in the genus Tragopogon. American Journal of Botany 37: 487-499.
78. OWNBEY, M., AND G. D. MCCOLLUM. 1954. The chromosomes of Tragopogon. Rhodora 56: 7-21.
79. OZKAN, H., A. A. LEVY, AND M. FELDMAN. 2001. Allopolyploidy-induced rapid genome evolution in the wheat (Aegilops-Triticum) group. Plant Cell 13: 1735-1747.
80. PALMER, D. K., K. O'DAY, H. L. TRONG, H. CHARBONNEAU, AND R. L. MARGOLIS. 1991. Purification of the centromere-specific protein CENPA and demonstration that it is a distinctive histone. Proceedings of the National Academy of Sciences, USA 88: 3734-3738.
81. PARIDA, A., S. N. RAINA, AND R. K. J. NARAYAN. 1990. Quantitative DNA variation between and within chromosome complements of Vigna species (Fabaceae). Genetica 82: 125-133.
82. PEDROSA, A., M. F. JANTSCH, E. A. MOSCONE, P. F. AMBROS, AND D. SCHWEIZER. 2001. Characterisation of pericentromeric and sticky intercalary heterochromatin in Ornithogalum longibracteatum (Hyacinthaceae). Chromosoma 110: 203-213.
83. PIKAARD, C. S. 2000. The epigenetics of nucleolar dominance. Trends in Genetics 16: 495-500.
84. PIRES, J. C., J. ZHAO, M. E. SCHRANZ, E. J. LEON, P. A. QUIJADA, L. N. LUKENS, AND T. C. OSBORN. In press. Flowering time divergence and genomic rearrangements in resynthesized Brassica polyploids (Brassicaceae). Biological Journal of the Linnean Society.
85. PRESTING, G. G., L. MALYSHEVA, J. FUCHS, AND I. SHUBERT. 1998. A Ty3/gypsy retrotransposon-like sequence localizes to the centromeric regions of cereal chromosomes. Plant Journal 16: 721-728.
86. PRICE, H. J., AND K. BACHMANN. 1975. DNA content and evolution in the Microseridinae. American Journal of Botany 62: 262-267.
87. RAJAGOPAL, J., S. DAS, D. K. KHURANA, P. S. SRIVASTAVA, AND M. LAKSHMIKUMARAN. 1999. Molecular characterization and distribution of a 145-bp tandem repeat family in the genus Populus. Genome 42: 909-918.
88. RAMSEY, J., AND D. W. SCHEMSKE. 2002. Neopolyploidy in flowering plants. Annual Reviews in Ecology and Systematics 33: 589-639.
89. SAMUEL, R., T. F. STUESSY, K. TREMETSBERGER, C. M. BAEZA, AND S. SILJAK-YAKOVLEV. 2003. Phylogenetic relationships among species of Hypochaeris (Asteraceae, Cichorieae) based on ITS, plastid trnL intron, trnL-F spacer, and matK sequences. American Journal of Botany 90: 496-507.
90. SAUNDERS, V. A., AND A. HOUBEN. 2001. The pericentromeric heterochromatin of the grass Zingeria biebersteiniana (2n = 4) is composed of Zbcen1-type tandem repeats that are intermingled with accumulated dispersedly organized sequences. Genome 44: 955-961.
91. SCHMIDT, T., AND J. S. HESLOP-HARRISON. 1998. Genomes, genes and junk: the large-scale organization of plant chromosomes. Trends in Plant Science 3: 195-199.
92. SCHWARZACHER, T., AND J. S. HESLOP-HARRISON. 2000. Practical in situ hybridization. Oxford Press, Oxford, UK.
93. SHAKED, H., K. KASHKUSH, H. OZKAN, M. FELDMAN, AND A. A. LEVY. 2001. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell 13: 1749-1759.
94. SINGH, R. J. 2003. Plant cytogenetics, 2nd ed. CRC Press, New York, New York, USA.
95. SKALICKA, K., K. Y. LIM, R. MATYÁSEK, B. KOUKALOVA, A. R. LEITCH, AND A. KOVARÍK. 2003. Rapid evolution of parental rDNA in synthetic tobacco allotetraploid line. American Journal of Botany 90: 988-996.
96. SMITH, J. F., K. H. SYTSMA, J. S. SHOEMAKER, AND R. L. SMITH. 1991. A qualitative comparison of total cellular DNA extraction protocols. Phytochemical Bulletin 23: 2-9.
97. SOKAL, R. R., AND F. J. ROHLF. 1980. Biometry, 2nd ed. W. H. Freeman, San Francisco, California, USA.
98. SOLOMON, M. J., F. STRAUSS, AND A. A. VARSHAVSKY. 1986. A mammalian high mobility group protein recognizes any stretch of six AT base pairs in duplex DNA. Proceedings of the National Academy of Sciences, USA 83: 1276-1280.
99. SOLTIS, D. E., AND P. S. SOLTIS. 1989. Allopolyploid speciation in Tragopogon: insights from chloroplast DNA. American Journal of Botany 76: 1119-1124.
100. SOLTIS, D. E., AND P. S. SOLTIS. 1999. Polyploidy: recurrent formation and genome evolution. Trends in Ecology and Evolution 14: 348-352.
101. SOLTIS, D. E., P. S. SOLTIS, J. C. PIRES, A. KOVARÍK, AND J. TATE. In press. Recent and recurrent polyploidy in Tragopogon (Asteraceae): genetic, genomic, and cytogentic Comparisons. Biological Journal of the Linnean Society.
102. SOLTIS, D. E., P. S. SOLTIS, AND J. A. TATE. 2004. Advances in the study of polyploidy since Plant Speciation. New Phytologist 161: 173-191.
103. SOLTIS, P. S., G. M. PLUNKETT, S. J. NOVAK, AND D. E. SOLTIS. 1995. Genetic variation in Tragopogon species: additional origins of the allotetraploids T. mirus and T. miscellus (Compositae). American Journal of Botany 82: 1329-1341.
104. SOLTIS, P. S., AND D. E. SOLTIS. 1991. Multiple origins of the allotetraploid Tragopogon mirus (Compositae): rDNA evidence. Systematic Botany 16: 407-413.
105. SOLTIS, P. S., AND D. E. SOLTIS. 2000. The role of genetic and genomic attributes in the success of polyploids. Proceedings of the National Academy of Sciences, USA 97: 7051-7057.
106. SONG, K., P. LU, K. TANG, AND T. C. OSBORN. 1995. Rapid genome change in synthetic polyploids of Brassica and its implications for polyploid evolution. Proceedings of the National Academy of Sciences, USA 97: 7051-7057.
107. STEBBINS, G. L. 1950. Variation and evolution in plants. Columbia University Press, New York, New York, USA.
108. STEBBINS, G. L. 1971. Chromosomal evolution in higher plants. Addison-Wesley, New York, New York, USA.
109. TAHARA, M. 1915. Cytological studies on Chrysanthemum. II. Botanical Magazine Tokyo 29: 48-50.
110. TORRELL, M., M. CERBAH, S. SILJAK-YAKOVLEV, AND J. VALLES. 2003. Molecular cytogenetics of the genus Artemisia (Asteraceae, Anthemideae): fluorochrome banding and fluorescence in situ hybridization. I. Subgenus Seriphidium and related taxa. Plant Systematics and Evolution 239: 141-153.
111. TORRELL, M., AND J. VALLES. 2001. Genome size in 21 Artemisia L. species (Asteraceae, Anthemideae): systematic, evolutionary, and ecological implications. Genome 44: 231-238.
112. URBANSKA, K. M., H. HURKA, E. LANDOLT, B. NEUFFER, AND K. MUMMENHOFF. 1997. Hybridization and evolution in Cardamine (Brassicaceae) at Urnerboden, central Switzerland: biosystematic and molecular evidence. Plant Systematics and Evolution 204: 233-256.
113. VENKATESWARLU, K., S.-W. LEE, AND R. N. NAZAR. 1991. Conserved upstream sequence elements in plant 5S ribosomal RNA-encoding genes. Gene 105: 249-253.
114. VERSHININ, A. V., E. G. ALKIMOVA, AND J. S. HESLOP-HARRISON. 1996. Molecular diversification of tandemly organized DNA sequences and heterochromatic chromosome regions in some Triticeae species. Chromosome Research 4: 517-525.
115. VERSHININ, A. V., AND J. S. HESLOP-HARRISON. 1998. Comparative analysis of the nucleosomal structure of rye, wheat, and their relatives. Plant Molecular Biology 36: 149-161.
116. VICIENT, C. M., A. SUONIEMI, K. ANAMTHAMAT-JONSSON, J. TANSKANEN, A. BEHARAV, E. NEVO, AND A. H. SCHULMAN. 1999. Retrotransposon BARE-1 and its role in genome evolution in the genus Hordeum. Plant Cell 11: 1769-1784.
117. VOLKOV, R. A., N. V. BORISJUK, I. I. PANCHUK, D. SCHWEIZER, AND V. HEMBELEBEN. 1999. Elimination and rearrangement of parental rDNA in the allotetraploid Nicotiana tabacum. Molecular Biology and Evolution 16: 311-320.
118. VOS, P., R. HOGERS, M. BLEEKER, M. REIJANS, T. VAN DE LEE, M. HORNES, A. FRIJTERS, J. POT, J. PELEMAN, M. KUIPER, AND M. ZABEAU. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407-4414.
119. WEISS, H., T. F. STUESSY, J. GRAU, AND C. M. BAEZA. 2003. Chromosome reports from South American Hypochaeris (Asteraceae). Annals of the Missouri Botanical Garden 90: 56-63.
120. WENDEL, J. F. 2000. Genome evolution in polyploids. Plant Molecular Biology 42: 225-249.
121. WENDEL, J. F., A. SCHNABEL, AND T. SEELANAN. 1995. An unusual ribosomal DNA-sequence from Gossypium gossypoioides reveals ancient, cryptic, intergenomic introgression. Molecular Phylogenetics and Evolution 4: 298-313.
122. WIENS, G. R., AND P. K. SORGER. 1998. Centromeric chromatin and epigenetic effects in kinetochore assembly. Cell 93: 313-316.
123. ZHANG, P., B. FRIEBE, A. J. LUKASZEWSKI, AND B. S. GILL. 2001. The centromere structure in Robertsonian wheat-rye translocation chromosomes indicates that centric breakage-fusion can occur at different positions within the primary constriction. Chromosoma 110: 335-344.
124. ZHAO, X.-P., Y. SI, R. E. HANSON, C. F. CRANE, H. J. PRICE, D. M. STELLY, J. F. WENDEL, AND A. H. PATERSON. 1998. Dispersed repetitive DNA has spread to new genomes since polyploid formation in cotton. Genome Research 8: 479-492.