Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize lines: Bt11, MON810, T25, and GA21

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 9, 2005, Pages 3333-3337

  Hernández, Maria ^a,d   Rodríguez Lázaro, David ^b,e   Zhang, David ^c   Esteve, Teresa ^a   Pla, Maria ^a,d   Prat, Salomé ^a  

^a CSIC   (Spain)

^b UNIVERSITY OF GIRONA   (Spain)

^c BioGEVES   (France)

^d INSTITUTO TECNOLÓGICO AGRARIO DE CASTILLA Y LEÓN   (Spain)

^e UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Bt11; GA21; GMO; Maize; MON810; Multiplex PCR; T25

Indexed keywords

PLANT DNA;

ARTICLE; CHEMISTRY; GENETICS; MAIZE; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSGENIC PLANT;

BASE SEQUENCE; DNA, PLANT; MOLECULAR SEQUENCE DATA; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; ZEA MAYS;

ZEA MAYS;

EID: 19944420150     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf049192y     Document Type: Article

References (35)

1. Clive, J. Preview: Global status of commercialized biotech/GM crops: 2004. ISAAA briefs no. 32; ISAAA: Ithaca, NY, 2004.
2. Regulation (EC) no. 258/97 of the European Parliament and the Council of 27 January 1997 concerning novel foods and novel food ingredients. Off. J. Eur. Communities: Legis. 1997, L43, 1-7.
3. Regulation (EC) no. 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Off. J. Eur. Communities: Legis. 2003, L268, 1-23.
4. Regulation (EC) no. 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending directive 2001/18/EC. Off. J. Eur. Communities: Legis. 2003, L268, 24-28.
5. Hernández, M.; Rodríguez-Lázaro, D.; Ferrando, A. Current methodology for detection, identification and quantification of genetically modified organisms. Curr. Anal. Chem., in press.
6. Hübner, P.; Waiblinger, H. U.; Pietsch, K.; Brodmann, P. Validation of PCR methods for quantitation of genetically modified plants in food. J. AOAC Int. 2001, 84, 1855-1864.
7. Wiseman, G. State of the art and limitations of quantitative polymerase chain reaction. J. AOAC Int. 2002, 85, 79279-79286.
8. Meyer, R. Nachweis gentechnologisch veränderte Pflanzen mittels der Polymerase Kettenreaktion (PCR) am Beispiel der FLAVR SAVR™- Tomate. Z. Lebesnsm.-Unters. -Forsch. A 1995, 201, 583-586.
9. Weiblinger, H. U.; Wurz, A.; Freyer, R.; Pietsch, K. Spezifischer Nachweis von gentechnisch verändertem Raps in Honig. Dtsch. Lebensm.-Rundsch. 1999, 95, 44-48.
10. Ehlers, V. B.; Strauch, E.; Goltz, M.; Kubsch, D.; Wagner, H.; Miadhof, H.; Bendiek, J.; Appel, B.; Buhk, H. J. Nachweis gentechnischer veränderugen in mais mittels PCR. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 1997, 4, 118-121.
11. Studer, E.; Dahinden, I.; Lüthy, J.; Hübner, P. Nachweis des gentechnisch veränderten "Maximizer"-mais mittels der Polymerase-Kettenreaktion (PCR). Mitt. Gebiete Lebensm. Hyg. 1997, 88, 515-524.
12. Hupfer, C.; Hotzel, H.; Sachse, K.; Engel, K. H. Detection of genetically modified insect-resitant Bt maize by means of polymerase chain reaction. Z. Lebensm.-Unters. -Forsch. 1997, 205, 442-445.
13. Zimmermann, A.; Lüthy, J.; Pauli, U. Event specific transgene detection in Bt11 corn by quantitative PCR at the integration site. Lebensm.-Wiss. -Technol. 2000, 33, 210-216.
14. Padgette, S. R.; Kolacz, K. H.; Delannay, X.; Re, D. B.; Lavalee, B. J.; Tinius, C. N.; Rhodes, W. K.; Otero, Y. I.; Barry, G. F.; Eichholtz, D. A.; Peschke, V. M.; Nida, D. L.; Taylor, N. B.; Kishore, G. M. Development, identification and characterization of glyphosate-tolerant soybean line. Crop Sci. 1995, 35, 1451-1461.
15. Brodmann, P.; Eugster, A.; Hübner, P.; Meyer, R.; Pauli, U.; Vöegliand, U.; Lüthy, J. Nachweis gentechnisch veänderter Roundup Ready Sojabohnen mittels Polymerase-Kettenreaktion (PCR). Mitt. Gebiete Lebensm. Hyg. 1997, 88, 722-731.
16. Van Hoef, A. M. A.; Kok, E. J.; Bouw, E.; Kuiper, H. A.; Keijer, J. Development and application of a selective detection method for genetically modified soy and soy-derived products. Food Addit. Contam. 1998, 15, 767-774.
17. Koppel, E.; Stadler, M.; Lüthy, J.; Hübner, P. Sensitive method for the detection of the genetically engineered soybean Roundup Ready. Mitt. Gebiete Lebensm. Hyg. 1997, 88, 164-175.
18. Zimmermann, S.; Zehner, R.; Mebs, D. Tierartenidentifizierung aus fleischproben mittels DNA-analyse. Fleischwirtschaft 1998, 78, 530-533.
19. Hernández, M.; Pla, M.; Esteve, T.; Prat, S.; Puigdomènech, P.; Ferrando, A. A. specific real-time quantitative PCR detection system for event MON810 in maize YieldGard® based on the 3′-transgene integration sequence. Transgenic Res. 2003, 12, 179-189.
20. Hernández, M.; Esteve, T.; Prat, S.; Pla, M. Development of real-time PCR systems based on SYBR® Green I, Amplifluor™ and TaqMan® technologies for specific quantitative detection of transgenic maize event GA21. J. Cereal Sci. 2004, 39, 99-107.
21. Nielsen, C. R.; Berdal, K. G.; Holst-Jensen, A. Characterisation of the 5′ integration site and development of an event-specific real-time PCR assay for NK603 maize from a low starting copy number Eur. Food Res. Technol. 2004, 219, 421-427.
22. Windels, P.; Bertrand, S.; Depicker, A.; Moens, W.; Van Bockstaele, E.; De Loose, M. Qualitative and event-specific PCR real time detection methods for StarLink maize. Eur. Food Res. Technol. 2003, 216, 259-263.
23. Holst-Jensen, A.; Rønning, S. B.; Lovseth, A.; Berdal, K. G. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 2003, 375, 985-993.
24. Markoulatos, P.; Siafakas, N.; Moncany, M. J. Multiplex polymerase chain reaction: a practical approach. J. Clin. Lab. Anal. 2002, 16, 47-51.
25. Matsuoka, T.; Kuribara, H.; Akiyama, H.; Miura, H.; Goda, Y.; Kusukabe, Y.; Isshiki, K.; Toyoda, M.; Hino, A. A Multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize. J. Food Hyg. Soc. Jpn. 2001, 42, 28-32.
26. Permingeat, H. R.; Reggiardo, M. I.; Vallejos, R. H. Detection and quantification of transgenes in grains by multiplex and real-time PCR. J. Agric. Food Chem. 2002, 50, 4431-4436.
27. Brodmann, P.; Ilg, EC.; Berthoud, H.; Herrmann, A. Real-time quantitative polymerase chain reaction methods for four genetically modified maize varieties and maize DNA content in food. J. AOAC Int. 2002, 85, 646-53.
28. Rogers, S. O.; Bendich, A. J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 1985, 5, 69-76.
29. Huang, H.-Y.; Pan, T. M. Detection of genetically modified maize MON810 and NK603 by multiplex and real-time polymerase chain reaction methods. J. Agric. Food Chem. 2004, 52, 3264-3268.
30. Germini, A.; Zanetti, A.; Salati, C.; Rossi, S.; Forré, C.; Schmid, S.; Marchelli, R. Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J. Agric. Food Chem. 2004, 52, 3275-3280.
31. Rho, J. K.; Lee, T.; Jung, S.-I.; Kim, T.-A.; Park, Y.-H.; Kim, Y.-M. Qualitative and quantitative PCR methods for detection of three lines of genetically modified potatoes. J. Agric. Food Chem. 2004, 52, 3269-3274.
32. Arumuganathan, K.; Earle, E. D. Nuclear DNA content of some important plant species. Plant Mol. Biol. Rep. 1991, 9, 208-218.
33. Puumalainen, J.; Paoletti, C.; Bertheau, Y.; Bonner, P.; Broil, H.; Damant, A.; De Santis, B.; Holst-Jensen, A., Laurensse, E.; Mønsted Jørgensen, M.; Onori, R.; Stephensen Lübeck, P.; Taverniers, I.; Trapmann, S.; Van den Eede, G. Definition of minimum performance requirements for analytical methods of GMO testing, ENGL WG Validation, version 1.7, 2003.
34. Wurz, A.; Willmund, R. Identification of transgenic glyphosate-resistant soybeans. In Food produced by means of genetic engeneering, 2nd status report; Schreiber, G. A., Bögl, K. W., Eds.; Bundesinstitut für Gesundheitlichen Verbraucherschutz und Veterinärmedizin, BgVV: Berlin, 1997.
35. BgVV (Federal Institute for Health Protection). Collection of official methods under article 35 of the German Federal Foods Act; methods of sampling and analysis of foods, tobacco products, cosmetics and commodity goods. Federal Health Office, Beuth Verlag GmbH, 1999, L00, 00-31.