Utilization of target-specific, hypersensitive strains of Saccharomyces cerevisiae to determine the mode of action of antifungal compounds

Antimicrobial Agents and Chemotherapy

Volumn 49, Issue 6, 2005, Pages 2558-2560

  Buurman, Ed T ^a   Andrews, Beth ^a   Blodgett, April E ^a   Chavda, Jini S ^a   Schnell, Norbert F ^a,b  

^a ASTRAZENECA   (United Kingdom)

^b Fachhochschule Aalen   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIFUNGAL AGENT; AUREOBASIDIN A; ERG7P INHIBITOR; FLUCONAZOLE; PROTEIN INHIBITOR; STAUROSPORINE; TERBINAFINE; THERMOZYMOCIDIN; UNCLASSIFIED DRUG; ZARAGOZIC ACID A;

ANTIFUNGAL ACTIVITY; ARTICLE; DRUG MECHANISM; DRUG SENSITIVITY; DRUG TARGETING; ERG7P GENE; GENE; HUMAN; HYPERSENSITIVITY; IC 50; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SCREENING TEST; STRAIN DIFFERENCE; TISSUE SPECIFICITY;

ANTIFUNGAL AGENTS; LANOSTEROL; MICROBIAL SENSITIVITY TESTS; PROTEIN KINASE C; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; STEROLS;

EID: 19544370025     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.49.6.2558-2560.2005     Document Type: Article

References (31)

1. Buurman, E. T., A. E. Blodgett, K. G. Hull, and D. Carcanague. 2004. Pyridines and pyrimidines mediating activity against an efflux-negative strain of Candida albicans through putative inhibition of lanosterol demethylase. Antimicrob. Agents Chemother. 48:313-318.
2. Chen, D. Z., D. V. Patel, C. J. Hackbarth, W. Wang, G. Dreyer, D. C. Young, P. S. Margolis, C. Wu, Z. Ni, J. Trias, R. J. White, and Z. Yuan. 2000. Actinonin, a naturally occurring antibacterial agent, is a potent deformylase inhibitor. Biochemistry 39:1256-1262.
3. Chen, J. K., W. S. Lane, and S. L. Schreiber. 1999. The identification of myriocin-binding proteins. Chem. Biol. 6:221-235.
4. Corey, E. J., S. P. Matsuda, and B. Bartel. 1994. Molecular cloning, characterization, and overexpression of ERG7, the Saccharomyces cerevisiae gene encoding lanosterol synthase. Proc. Natl. Acad. Sci. USA 91:2211-2215.
5. Devito, J. A., J. A. Mills, V. G. Liu, A. Agarwal, C. F. Sizemore, Z. Yao, D. M. Stoughton, M. Grazia Cappiello, M. D. F. S. Barbosa, L. A. Foster, and D. L. Pompliano. 2002. An array of target-specific screening strains for antibacterial drug discovery. Nat. Biotechnol. 20:478-483.
6. Fonzi, W. A., and M. Y. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717-728.
7. Goldman, R. C., D. Zakula, J. O. Capobianco, B. A. Sharpe, and J. H. Griffin. 1996. Inhibition of 2,3-oxidosqualene-lanosterol cyclase in Candida albicans by pyridinium ion-based inhibitors. Antimicrob. Agents Chemother. 40:1044-1047.
8. Heidler, S. A., and J. A. Radding. 1995. The AUR1 gene in Saccharomyces cerevisiae encodes dominant resistance to the antifungal agent aureobasidin A (LY295337). Antimicrob. Agents Chemother. 39:2765-2769.
9. Jandrositz, A., F. Turnowsky, and G. Hogenauer. 1991. The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization. Gene 107:155-160.
10. Jennings, S. M., Y. H. Tsay, T. M. Fisch, and G. W. Robinson. 1991. Molecular cloning and characterization of the yeast gene for squalene synthetase. Proc. Natl. Acad. Sci. USA 88:6038-6042.
11. Johnson, M., and R. W. Davis. 1984. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:1440-1448.
12. Kalb, V. F., C. W. Woods, T. G. Turi, C. R. Dey, T. R. Sutler, and J. C. Loper. 1987. Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA 6:529-537.
13. Kelly, S. L., D. C. Lamb, A. J. Corran, B. C. Baldwin, and D. E. Kelly. 1995. Mode of action and resistance to azole antifungals associated with the formation of 14 α-methylergosta-8,24(28)-dien-3β,6α-diol. Biochem. Biophys. Res. Commun. 207:910-915.
14. 1 progression. Cell 73: 585-596.
15. Levin, D. E., F. O. Fields, R. Kunisawa, J. M. Bishop, and J. Thorner. 1990. A candidate protein kinase C gene, PKC1, is required for the Saccharomyces cerevisiae cell cycle. Cell 62:213-224.
16. Ling, L. L., J. Xian, S. Ali, B. Geng, J. Fan, D. M. Mills, A. C. Arvanites, H. Orgueira, M. A. Ashwell, G. Carmel, Y. Xiang, and D. T. Moir. 2004. Identification and characterization of inhibitors of bacterial enoyl-acyl carrier protein reductase. Antimicrob. Agents Chemother. 48:1541-1547.
17. Longtine, M. S., A. McKenzie III, D. J. Demarini, N. G. Shah, A. Wach, A. Brachat, P. Philippsen, and J. R. Pringle. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14:953-961.
18. Munich Information Center for Protein Sequences [Online.] http://mips.gsf.de.
19. Nagiec, M. M., E. E. Nagiec, J. A. Baltisberger, G. B. Wells, R. L. Lester, and R. C. Dickson. 1997. Sphingolipid synthesis as a target for antifungal drugs. Complementation of the inositol phosphorylceramide synthase defect in a mutant strain of Saccharomyces cerevisiae by the AUR1 gene. J. Biol. Chem. 272:9809-9817.
20. Nagiec, M. M., J. A. Baltisberger, G. B. Wells, R. L. Lester, and R. C. Dickson. 1994. The LCB2 gene of Saccharomyces and the related LCB1 gene encode subunits of serine palmitoyltransferase, the initial enzyme in sphingolipid synthesis. Proc. Natl. Acad. Sci. USA 91:7899-7902.
21. National Committee for Clinical Laboratory Standards. 1997. Reference method for broth microdilution antifungal susceptibility testing of yeasts: approved standard. Document M27-A. National Committee for Clinical Laboratory Standards, Wayne, Pa.
22. Ponpipom, M. M., N. N. Girotra, R. L. Bugianesi, C. D. Roberts, G. D. Berger, R. M. Burk, R. W. Marquis, W. H. Parsons, K. F. Bartizal, J. D. Bergstom, M. M. Kurtz, J. C. Onishi, and D. J. Rew. 1994. Structure-activity relationships of C1 and C6 side chains of zaragozic acid A derivatives. J. Med. Chem. 37:4031-4051.
23. Ryder, N. S. 1985. Specific inhibition of fungal sterol biosynthesis by SF 86-327, a new allylamine antimycotic agent. Antimicrob. Agents Chemother. 27:252-256.
24. Ryder, N. S. 1992. Terbinafine: mode of action and properties of the squalene epoxidase inhibition. Br. J. Dermatol. 126(Suppl. 39):2-7.
25. Ryder, N. S., G. Seidl, and P. F. Troke. 1984. Effect of the antimycotic drug naftifine on growth and sterol biosynthesis in Candida albicans. Antimicrob. Agents Chemother. 25:483-487.
26. Saccharomyces Genome Database [Online.] http://www.yeastgenome.org.
27. Sanglard, D., F. Ischer, M. Monond, and J. Bille. 1997. Cloning of Candida albicans genes conferring resistance to azole antifungals: characterization of CDR2, a new multidrug ABC transporter gene. Microbiology 143:405-416.
28. Slater-Radosti, C., G. Van Aller, R. Greenwood, R. Nicholas, P. M. Keller, W. E. DeWolf, Jr., F. Fan, D. J. Payne, and D. D. Jaworski. 2001. Biochemical and genetic characterization of the action of triclosan on Staphylococcus aureus. J. Antimicrob. Chemother. 48:1-6.
29. Tsay, Y. H., and G. W. Robinson. 1991. Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase. Mol. Cell. Biol. 11:620-631.
30. Vanden Bossche, H., P. Marichal, and F. C. Odds. 1995. Molecular mechanisms of drug resistance in fungi. Trends Microbiol. 2:393-400.
31. Yoshida, S., E. Ikeda, I. Uno, and H. Mitsuzawa. 1992. Characterization of a staurosporine- and temperature-sensitive mutant, stt1, of Saccharomyces cerevisiae: STT1 is allelic to PKC1. Mol. Gen. Genet. 231:337-344.