Characterization of two novel pacifastin-like peptide precursor isoforms in the desert locust (Schistocerca gregaria): cDNA cloning, functional analysis and real-time RT-PCR gene expression studies

Biochemical Journal

Volumn 388, Issue 1, 2005, Pages 281-289

  Simonet, Gert ^a   Bredgelmans, Bert ^a   Proost, Paul ^a   Claeys, Ilse ^a   Van Damme, Jozef ^a   De Loof, Arnold ^a   Vanden Broeck, Jozef ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

Pacifastin like peptide precursor; Phase polymorphism; Protease inhibitor; Schistocerca gregaria (desert locust); Serine peptidase; Transcript profiling

Indexed keywords

ARTHROPODS; PEPTIDE SEQUENCES; POLYMORPHISM; SERINE PROTEASE INHIBITORS;

BIOCHEMISTRY; BRAIN; CLONING; DNA; GENES; PURIFICATION; RNA; SILICA;

ENZYMES;

COMPLEMENTARY DNA; PACIFASTIN; PROTEIN PRECURSOR; PROTEIN SGPI5; PROTEIN SGPP 4; PROTEINASE INHIBITOR; TRYPSIN; UNCLASSIFIED DRUG;

AEDES AEGYPTI; AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; FUNCTIONAL ASSESSMENT; GENE EXPRESSION; LIGHT CHAIN; LOCUST; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION; GRASSHOPPERS; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN ISOFORMS; PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT;

AEDES AEGYPTI; ARTHROPODA; ASTACOIDEA; BOVINAE; INSECTA; ORTHOPTERA; SCHISTOCERCA GREGARIA;

EID: 19544365384     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041414     Document Type: Article

References (41)

1. Iwanaga, S. (2002) The molecular basis of innate immunity in the horseshoe crab Curr. Opin. immunol. 14, 87-95
2. Leclerc, V. and Reichhart, J. M. (2004) The immune response of Drosophila melanogaster. Immunol. Rev. 198, 59-71
3. Bode, W. and Huber, R. (1992) Natural protein proteinase inhibitors and their interaction with proteinases. Eur. J. Biochem. 204, 433-451
4. Laskowski, M. and Qasim, M. A. (2000) What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme substrate complexes? Biochim. Biophys. Acta 1477, 324-337
5. Simonet, G., Claeys, I. and Vanden Broeck, J. (2002) Structural and functional properties of a novel serine protease inhibiting peptide family in arthropods. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 132, 247-255
6. Hergenhahn, H. G., Aspan, A. and Soderhall, K. (1987) Purification and characterization of a high-Mr proteinase inhibitor of pro-phenol oxidase activation from crayfish plasma. Biochem. J. 248, 223-228
7. Liang, Z., Sottrup-Jensen, L., Aspan, A., Hall, M. and Soderhall, K. (1997) Pacifastin, a novel 155-kDa heterodimeric proteinase inhibitor containing a unique transferrin chain. Proc. Natl. Acad. Sci. U.S.A. 94, 6682-6687
8. Boigegrain, R. A., Mattras, H., Brehelin, M., Paroutaud, P. and Coletti-Previero, M. A. (1992) Insect immunity: two proteinase inhibitors from hemolymph of Locusta migratoria. Biochem. Biophys. Res. Commun. 189, 790-793
9. Nakakura, N., Hietter, H., Van Dorsselaer, A. and Luu, B. (1992) Isolation and structural determination of three peptides from the insect Locusta migratoria: identification of a deoxyhexose-linked peptide. Eur. J. Biochem. 204, 147-153
10. Kellenberger, C., Boudier, C., Bermudez, I., Bieth, J. G., Luu, B. and Hietter, H. (1995) Serine protease inhibition by insect peptides containing a cysteine knot and a triple-stranded β-sheet. J. Biol. Chem. 270, 25514-25519
11. Hamdaoui, A., Wataleb, S., Devreese, B., Chiou, S. J., Vanden Broeck, J., Van Beeumen, J., De Loof, A. and Schoofs, L. (1998) Purification and characterization of a group of five novel peptide serine protease inhibitors from ovaries of the desert locust, Schistocerca gregaria. FEBS Lett. 422, 74-73
12. Schechter, I. and Berger, A. (1967) On the size of the active site in proteases. I. Papain. Biochem. Biophys. Res. Commun. 27, 157-162
13. Malik, Z., Amir, S., Pal, G., Buzas, Z., Varallyay, E., Antal, J., Szilagyi, Z., Vekey, K., Asboth, B., Patthy, A. and Graf, L. (1999) Proteinase inhibitors from desert locust, Schistocerca gregaria: engineering of both P(1) and P(1)' residues converts a potent chymotrypsin inhibitor to a potent trypsin inhibitor. Biochim. Biophys. Acta 1434, 143-150
14. Patthy, A., Amir, S., Malik, Z., Bodi, A., Kardos, J., Asboth, B. and Graf, L. (2002) Remarkable phylum selectivity of a Schistocerca gregaria trypsin inhibitor: the possible role of enzyme-inhibitor flexibility. Arch. Biochem. Biophys. 398, 179-187
15. Mer, G., Hietter, H., Kellenberger, C., Renatus, M., Luu, B. and Lefevre, J. F. (1996) Solution structure of PMP-C: a new fold in the group of small serine proteinase inhibitors. J. Mol. Biol. 258, 158-171
16. Kellenberger, C., Ferrat, G., Leone, P., Darbon, H. and Roussel, A. (2003) Selective inhibition of trypsins by insect peptides: role of P6-P10 loop. Biochemistry 42, 13605-13612
17. Mer, G., Kellenberger, C., Koehl, P., Stole, R., Sorokine, O., Van Dorsselaer, A., Luu, B., Hietter, H. and Lefevre, J. F. (1994) Solution structure of PMP-D2, a 35-residue peptide isolated from the insect Locusta migratoria. Biochemistry 33, 15397-15407
18. Roussel, A., Mathieu, M., Dobbs, A., Luu, B., Cambillau, C. and Kellenberger, C. (2001) Complexation of two proteic insect inhibitors to the active site of chymotrypsin suggests decoupled roles for binding and selectivity. J. Biol. Chem. 276, 33893-38898
19. Gaspari, Z., Patthy, A., Graf, L. and Perczel, A. (2002) Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria. Eur. J. Biochem. 269, 527-537
20. Kromer, E., Nakakura, N. and Lagueux, M. (1994) Cloning of a Locusta cDNA encoding a precursor peptide for two structurally related proteinase inhibitors. Insect Biochem. Mol. Biol. 24, 329-331
21. Simonet, G., Claeys, I., Van Soest, S., Breugelmans, B., Franssens, V., De Loof, A. and Vanden Broeck, J. (2004) Molecular identification of SGPP-5, a novel pacifastin-like peptide precursor in the desert locust. Peptides 25, 941-950
22. Simonet, G., Claeys, I., November, T., Wataleb, S., Janssen, T., Maes, R., De Loof, A. and Vanden Broeck, J. (2002) Cloning of two cDNAs encoding isoforms of a pacifastin-related precursor polypeptide in the desert locust, Schistocerca gregaria: analysis of stage- and tissue-dependent expression. Insect Mol. Biol. 11, 353-360
23. Simonet, G., Claeys, I., Vanderperren, H., November, T., De Loof, A. and Vanden Broeck, J. (2002) cDNA cloning of two different serine protease inhibitor precursors in the migratory locust, Locusta migratoria. Insect Mol. Biol. 11, 249-256
24. Vanden Broeck, J., Chiou, S. J., Schoofs, L., Hamdaoui, A., Vandenbussche, F., Simonet, G., Wataleb, S. and De Loof, A. (1998) Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression. Eur. J. Biochem. 254, 90-95
25. Parkinson, N. M., Conyers, C., Keen, J., MacNicoll, A., Smith, I., Audsley, N. and Weaver, R. (2004) Towards a comprehensive view of the primary structure of venom proteins from the parasitold wasp Pimpla hypochondriaca. Insect Biochem. Mol. Biol. 34, 565-571
26. Simonet, G., Claeys, I., Franssens, V., De Loof, A. and Vanden Broeck, J. (2003) Genomics, evolution and biological functions of the pacifastin peptide family: a conserved serine protease inhibitor family in arthropods. Peptides 24, 1633-1544
27. Gaspari, Z., Ortutay, C. and Perczel, A. (2004) A simple fold wild variations: the pacifastin inhibitor family Bioinformatics 20, 448-451
28. Simonet, G., Claeys, I., Breugelmans, B., Van Soest, S., De Loof, A. and Vanden Broeck, J. (2004) Transcript profiling of pacifastin-like peptide precursors in crowd- and isolated-reared desert locusts. Biochem. Biophys. Res. Comm. 317, 565-569
29. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
30. Claeys, I., Simonet, G., Van Loy, T., De Loof, A. and Vanden Broeck, J. (2003) cDNA cloning and transcript distribution of two novel members of the neuroparsin family in the desert locust, Schistocerca gregaria. Insect Mol. Biol. 12, 473-481
31. Janssen, T., Claeys, I., Simonet, G., De Loof, A., Girardie, J. and Vanden Broeck, J. V. (2001) cDNA cloning and transcript distribution of two different neuroparsin precursors in the desert locust, Schistocerca gregaria. Insect Mol. Biol. 10, 183-189
32. Nielsen, H., Engelbrecht, J., Brunak, S. and vonHeijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1-6
33. Bartholomay, L. C., Cho, W. L., Rocheleau, T. A., Boyle, J. P., Beck, E. T., Fuchs, J. F., Liss, P., Rusch, M., Butler, K. M., Wu, R. C. et al. (2004) Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus. Infect. Immun. 72, 4114-4126
34. Bolshakov, V. N., Topalis, P., Blass, C., Kokoza, E., della Torre, A., Kafatos, F. C. and Louis, C. (2002) A comparative genomic analysis of two distant diptera, the fruit fly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae. Genome Res. 12, 57-66
35. Vanden Broeck, J. (2001) Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster. Peptides 22, 241-254
36. Szenthe, B., Gaspari, Z., Nagy, A., Perczel, A. and Graf, L. (2004) Same fold with different mobility: backbone dynamics of small protease inhibitors from the desert locust, Schistocerca gregaria. Biochemistry 43, 3376-3384
37. Krowarsch, D., Cierpicki, T., Jelen, F. and Otlewski, J. (2003) Canonical protein inhibitors of serine proteases. Cell. Mol. Life Sci. 60, 2427-2444
38. Simonet, G., Claeys, I., Huybrechts, J., De Loof, A. and Vanden Broeck, J. (2003) Bacterial production and purification of SGPI-1 and SGPI-2, two peptidic serine protease inhibitors from the desert ocust, Schistocerca gregaria. Protein Expression Purif. 31, 138-196
39. Rahman, M. M., Vanden Bosch, L. Baggerman, G., Clynen, E., Hens, K., Hoste, B., Meylaers, K., Vercammen, T., Schoofs, L., De Loof, A. and Breuer, M. (2002) Search for peptidic molecular markers in hemolymph of crowd- (gregarious) and isolated-reared (solitary) desert locusts, Schistocerca gregaria. Peptides 23, 1907-1914
40. Clynen, E., Stubbe, D., De Loof, A. and Schoofs, L. (2002) Peptide differential display: a novel approach for phase transition in locusts. Comp. Biochem. Physiol. B Biochem. Mol Biol. 132, 107-115
41. Aspan, A., Hall, M. and Soderhall, K. (1990) The effect of endogenous proteinase inhibitors on the prophenoloxidase activating enzyme, a serine proteinase from crayfish haemocytes. Insect Biochem. 20, 485-492