Bacterial composition of activated sludge - Importance for floc and sludge properties

Water Science and Technology

Volumn 49, Issue 10, 2004, Pages 51-58

  Nielsen, P H ^a   Thomsen, T R ^a   Nielsen, J L ^a  

^a AALBORG UNIVERSITY   (Denmark)

Author keywords

Activated sludge; Dewatering; FISH; Floc strength; Flocculation; Settling

Indexed keywords

BACTERIA; DEWATERING; FLOCCULATION; MOLECULAR BIOLOGY;

ACTIVATED SLUDGES; BACTERIAL COMPOSITION;

SEWAGE SLUDGE;

ACTIVATED SLUDGE; BACTERIUM; DEWATERING; FLOC;

ACTIVATED SLUDGE; ANAEROBIC CAPACITY; ANALYTIC METHOD; BACTERIAL COLONIZATION; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; CORRELATION ANALYSIS; FLOCCULATION; FUNCTIONAL ASSESSMENT; MOLECULAR BIOLOGY; NONHUMAN; REVIEW; SENSITIVITY ANALYSIS; SHEAR STRESS;

BACTERIA (MICROORGANISMS);

EID: 1942544855     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2004.0606     Document Type: Review

References (36)

1. Crocetti, G.R., Hugenholtz, P., Bond, P.L., Schuler, A., Keller, J., Jenkins, D. and Blackall, L.L. (2000). Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantification. Appl. Environ. Microbiol., 66(3), 1175-1182.
2. Daims, H., Nielsen, J.L., Nielsen, P.H., Schleifer, K.-H. and Wagner, M. (2001). In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in wastewater treament plants. Appl. Environ. Microbiol., 67(11), 5273-5284.
3. Frølund, B., Palmgren, R., Keiding, K. and Nielsen, P.H. (1996). Extraction of exopolymers from activated sludge using a cation exchange resin. Wat. Res., 30, 1749-1758.
4. Hesselmann, R.P.X., Werlen, C., Hahn, D., van der Meer, J.R. and Zehnder, A.J.B. (1999). Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge. Syst. Appl. Microbiol., 22, 454-465.
5. Higgins, M.J. and Novak, J.T. (1997). Characterization of exocellular protein and its role in bioflocculation. J. Environ. Eng., 123, 479-485.
6. Juretschko, S., Loy, A., Lehner, A. and Wagner, M. (2002). The microbial community composition of a nitrifying-denitrifying activated sludge from an industrial sewage treatment plant analyzed by the full-cycle rRNA approach. Syst. Appl. Microbiol., 25(1), 84-99.
7. Keiding, K., Wybrandt, L. and Nielsen, P.H. (2001). Remember the water - a comment on EPS-colligative properties. Wat. Sci. Tech.,43(6), 17-23.
8. Klausen, M.M. (2001). Reflocculation potential of activated sludge flocs by combined aeration and substrate addition. MSc thesis, Department of Environmental Engineering, Aalborg University, Denmark.
9. Klausen M.M., Thomsen T.R., Nielsen, J.L., Ramsing N.B. and Nielsen, PH. (2003) Variations in microcolony strength of probe-defined bacteria in activated sludge flocs. Submitted.
10. Kloeke, F. Van Ommen and Geesey, G.G. (1998). Localization and identification of populations of phosphatase-active bacterial cells associated with activated sludge flocs. Microbiol. Ecol., 38, 201-214.
11. Lajoie, C.A., Layton, A.C., Gregory, I.R., Sayler, G.S., Taylor, D.E. and Meyers, A.J. (2000). Zoogleal clusters and sludge dewatering potential in an industrial activated-sludge wastewater treatment plant. Wat. Environ. Res., 72,56-64:
12. Lavallée, B., Lessard, P. and Besser, C. (2002). Decay rate variability of active heterotrophic biomass. Wat. Sci. Tech., 46(1-2), 423-30.
13. Lee, N., Nielsen, P.H., Andreasen, K., Juretschko, S., Nielsen, J.L., Schleifer, K.-H. and Wagner, M. (1999). Combination of fluorescent in situ hybidization and microautoradiography - a new tool for structure-function analysis in microbial ecology. Appl. Environ. Microbiol., 65, 1289-1297.
14. Liu, Y. and Tay, J-H. (2002). The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge. Wat Res., 36, 1653-1665.
15. Manz, W., Eisenbrecher, M., Neu, T.R. and Szewzyk, U. (1998). Abundance and spatial organization of gram-negative sulfate-reducing bacteria in activated sludge investigated by in situ probing with specific 16S rRNA targeted oligonucleotides. FEMS. Microbiol. Ecol., 25,43-61.
16. Mikkelsen, L.H. and Keiding, K. (2002). Physico-chemical characteristics of full-scale sewage sludges with implications for dewatering. Wat. Res., 36, 2451-2462.
17. Neu, T.R., Swerhone, G.D.W. and Lawrence, J.R. (2001). Assessment of lectin-binding analysis for in situ detection of glycoconjugates in biofilm systems. Microbiol., 147, 299-313.
18. Nielsen, J.L. and Nielsen, P.H. (2002a). Quantification of functional groups in activated sludge by microautoradiography. War. Sci. Tech. 46(1-2), 389-395.
19. Nielsen, J.L. and Nielsen, P.H. (2002b). Enumeration of acetate-consuming bacteria by microautoradiography under oxygen- and nitrate respiring conditions in activated sludge. Wat. Res., 36, 421-428.
20. Nielsen, J.L., Mikkelsen, L.H. and Nielsen, P.H. (2001). In situ detection of cell surface hydrophobicity of probe-defined bacteria in activated sludge. Wat. Sci. Tech., 43(6), 97-103.
21. Nielsen, J.L., Juretschko, S., Wagner, M. and Nielsen, P.H. (2002a). Abundance and phylogenetic affiliation of iron reducers in activated sludge as assessed by fluorescence in situ hybridization and microautoradiography. Appl. Environ. Microbiol., 68, 4629-4636.
22. Nielsen, P.H. (2002). The activated sludge floc. In: Encyclopedia in Environmental Microbiology. G. Bitton (ed.), Chichester, England, Wiley.
23. Nielsen, P.H. and Keiding, K. (1998). Disintegration of activated sludge flocs in the presence of sulfide. Wat. Res., 32, 313-320.
24. Nielsen, P.H., Andreasen, K., Lee, N. and Wagner, M. (1999). Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge. Wat. Sci. Tech., 39, 1-9.
25. Nielsen, P.H. Aquino de Muro, M. and Nielsen, J.L. (2000). Studies on the in situ physiology of Thiothrix spp. in activated sludge. Environ. Microbiol., 2(4), 389-398.
26. Nielsen, P.H., Roslev, P., Dueholm, T. and Nielsen, J.L. (2002b). Microthrix parvicella, a specialized lipid consumer in anaerobic - aerobic activated sludge plants. Wat. Sci. Tech., 46(1-2), 73-80.
27. Purkhold, U., Pommerening-Röser, A., Juretschko, S., Koops, H.-P. and Wagner, M. (2000). Phylogeny of all recognized species of ammonia-oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys. Appl. Environ. Microbiol., 66, 5368-5382.
28. Schmid, M., Thill, A., Purkhold, U., Walcher, M., Bottero, J.Y., Ginestet, P., Nielsen, P.H., Wuertz, S. and Wagner, M. (2003). Characterization of activated sludge flocs by confocal laser scanning microscopy and image analysis. Wat. Res. 37, 2043-2052.
29. Serafim, L.S., Lemos, P.C., Levantesi, C., Tandoi, V., Santos, H. and Reis, M.A.M. (2002). Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms. J. Microbiol. Met., 51, 1-18.
30. Snaidr, J., Beimfort, C., Levantesi, C., Rossetti, S., Waarde, J. v. d., Geurkink, B., Lemaitre, M. and Tandoi, V. (2002). Phylogenetic analysis and in situ identification of "Nostocoida limicola"- like filamentous bacteria in activated sludge from industrial wastewater treatment plants. Wat. Sci. Tech., 46(1-2), 99-104.
31. Thomsen, T.R., Nielsen, J.L., Ramsing, N.B. and Nielsen, P.H. (2003). Micromanipulation and identification of FISH labelled micro-colonies in activated sludge. (Submitted).
32. Wagner, M. and Loy, A. (2002). Bacterial community composition and function in sewage treatment systems. Cur. Opin. Biotechnol., 13, 218-227.
33. Wagner, M., Amann, R., Lemmer, H. and Schleifer, K.-H. (1993). Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependend methods for describing microbial community structure. Appl. Environ. Microbiol., 59, 1520-1525.
34. Wilén, B.-M., Nielsen, J.L., Keiding, K. and Nielsen, P.H. (2000a). Influence of microbial activity on the stability of activated sludge flocs. Colloid & Surfaces: Biointerfaces, 18, 145-156.
35. Wilén, B.-M., Keiding, K. and Nielsen, P.H. (2000b). Activated sludge floc deflocculation during anaerobic conditions and reflocculation during aerobic conditions. Wat. Res., 34, 3933-3942.
36. Zita, A. and Hermansson, M. (1997). Determination of bacterial cell surface hydrophobicity of single cells in cultures and in wastewater in situ. FEMS Microbiol. Lett., 152, 299-306.